Consulta: creatorFacets:"Landgraf,T.N."
Registros recuperados: 1
Data/hora: 11/06/2024 14:41:53

Recombinant 60-kDa heat shock protein from Paracoccidioides brasiliensis: is it a good antigen for serological diagnosis of paracoccidioidomycosis?
Provedor de dados: 56
Autores: Peron,G.; Fernandes,F.F.; Landgraf,T.N.; Martinez,R.; Panunto-Castelo,A.
Paracoccidioides brasiliensis and P. lutzii are fungi that cause paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in South America. For serological diagnosis, although 43-kDa glycoprotein (gp43) is regarded as highly specific for PCM, the occurrence of false negative reactions in sera from patients infected with P. lutzii suggests that preparation with only one antigen is not recommended. Heat shock proteins are feasible alternatives as a second antigen because they are often highly immunogenic. In this study, we evaluated the usefulness of recombinant 60-kDa heat shock protein from P. brasiliensis (rPbHsp60) for the serological diagnosis of PCM. Using western blotting assay, we observed that 77.3% of the sera from PCM patients were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paracoccidioidomycosis; Heat shock protein; Serodiagnosis; ELISA; Western blotting.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400705