Consulta: creatorFacets:"Langoni,H."
Registros recuperados: 20
Data/hora: 02/06/2024 15:56:58

Rabies in the big fruit-esting bat Artibeus lituratus from Botucatu, Southeastern Brazil
Provedor de dados: 80
Autores: Langoni,H.; Lima,K.; Menozzi,B. D.; Silva,R. C.
Rabies is a viral disease of mammals transmitted through the bite of a rabid animal. A frugivorous adult male bat, Artibeus lituratus, family Phyllostomidae, was diagnosed as positive to rabies by direct immunofluorescence (DIF) and mouse inoculation test (MIT) of the bat's brain, both performed at the School of Veterinary Medicine and Animal Husbandry - FMVZ, UNESP, Botucatu, São Paulo State, Brazil. The animal collided with the window of a commercial establishment in the urban area during the day. With regard to DIF, a high amount of Negri bodies of several sizes was observed in the brain. The spleen and right kidney presented some Negri bodies too. In relation to MIT, the mice presented paralysis in the 7th day, and died in the day after with several...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Southeastern Brazil; Rabies; Phyllostomidae; Frugivorous bat.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100010

Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected
Provedor de dados: 80
Autores: Langoni,H.; Das Dores,C. B.; Silva,R. C.; Pezerico,S. B.; Castro,A. P. B.; Da Silva,A. V.
Detection of Toxoplasma gondii (T. gondii) DNA in blood can help to diagnose the disease in its acute phase; however, it must be considered that hemoglobin, present in blood, can inhibit polymerase activity, making impracticable the detection of DNA in samples. Mice were experimentally infected via oral route with ME49 and BTU2 strains cysts and RH strain tachyzoites; polymerase chain reaction was used to detect T. gondii DNA in mice sera 18, 24, 48, 96, and 192 hours post infection (PI). Toxoplama gondii DNA was detected in only one animal infected with BTU2 strain, genotype III (isolated from a dog with neurological signs) 18 hours PI. The agent's DNA was not detected in any sample of the other experimental groups. New studies must be carried out to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmosis; PCR; Serum; Experimental infection; Mice.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200004

Serological profile of anti-Toxoplasma gondii antibodies in apparently healthy dogs of the city of Botucatu, São Paulo State, Brazil
Provedor de dados: 80
Autores: Langoni,H.; Modolo,J. R.; Pezerico,S. B.; Silva,R. C.; Castro,A. P. B.; Silva,A. V. da; Padovani,C. R.
Toxoplasmosis is a zoonosis caused by Toxoplasma gondii, a coccidian protozoan of worldwide distribution. The seroprevalence in canine population can be an alternative for measuring T. gondii urban spreading. A total of 780 blood samples from dogs were collected, during the yearly anti-rabies campaign, carried out by the Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Husbandry (FMVZ), São Paulo State University, UNESP, together with the county health authorities, in August 1999. Using Indirect Fluorescent Antibody Test (IFAT) for detecting antibodies anti-T. gondii in the sera samples, we observed that 258 dogs (33.1%) were positive. The associations between the serological results and the epidemiological...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Toxoplasma gondii; Dog; Serology.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100012

Impact of leishmaniasis on public health
Provedor de dados: 80
Autores: Camargo,L. B; Langoni,H.
Leishmaniasis is a parasitic zoonosis caused by protozoans of the genus Leishmania transmitted by insects known as phlebotomines, which are found in wild or urban environments. It affects domestic and wild animals and transmission to man happens by accident. The disease occurs in tropical and sub-tropical areas, mainly in Asia, Europe, Africa, and the Americas. There are two forms that affect man: American cutaneous leishmaniasis (ACL) and American visceral leishmaniasis (AVL). The latter is caused by three species of Leishmania: Leishmania (Leishmania) donovani, Leishmania (Leishmania) infantum, and Leishmania (Leishmania) chagasi, which are grouped in the Leishmania (Leishmania) donovani complex. Wild reservoir hosts of L. chagasi known so far are foxes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Cutaneous leishmaniasis; Visceral leishmaniasis; Leishmania.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400002

Role of cytokines, NO, and H2O2 on the immunopathology of Leptospirosis in genetically selected mice
Provedor de dados: 80
Autores: Marinho,M.; Langoni,H.; Oliveira,S. L.; Lima,V. M. F.; Peiró,J. R.; Perri,S. H. V.; Carreira,R.
Immune response to leptospirosis is mainly humorally mediated, and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. However, some aspects are still unknown. For a more detailed analysis of the cellular immune response to leptospirosis infection, trials were carried out in order to determine the hydrogen peroxide and nitric oxide (H2O2 and NO) production stimulated or not by Interferon-gamma. The participation of some specific cytokines, such as Tumor Necrosis Factor-alpha (TNF-alpha); Interferon-gamma (IFN-gamma); Interleukin-6 (IL-6); and Interleukin-4 (IL-4), in the immunopathology of this infection was also investigated. For this purpose, we analyzed the supernatant from peritoneal macrophage cell culture and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Cytokines; Cellular immunity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200009

Anti-leptospirose agglutinins in equine sera, from São Paulo, Goias, and Mato Grosso do Sul, Brazil, 1996-2001
Provedor de dados: 80
Autores: Langoni,H.; Da Silva,A.V.; Pezerico,S.B.; De Lima,V.Y.
Equine leptospirosis can present a non-symptomatic form, an acute clinical form, or even develop chronically, causing reproductive alterations, such as abortion and recurrent uveitis. Since the prevalence of leptospirosis in several countries and regions is widely reported, the objective of this study was to verify the prevailing equine leptospirosis in different regions of Brazil. Sera from 1402 blood samples from horses of different age, sex, breed, and purpose were examined. These samples came from southeastern and central west states of Brazil. The method utilized was the Microscopic Agglutination Test (MAT), with 12 different Leptospira serovars. From the sera tested, 754 (54%) were positive for one (385) or more (372) serovars. These results were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Horse; Serology.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300003

Brazilian spotted fever: a reemergent zoonosis
Provedor de dados: 80
Autores: Greca,H.; Langoni,H.; Souza,L.C.
Brazilian spotted fever is caused by the bacterium Rickettsia rickettsii, which is the most pathogenic species of the spotted-fever rickettsiae group and is transmitted by the bite of infected ticks. Amblyomma cajennense is the most important tick species involved in the cycle of this zoonosis in Brazil as it presents low host specificity, great number of natural reservoirs and wide geographic distribution. It was first described in the state of São Paulo in 1929 and later in Rio de Janeiro, Minas Gerais and Bahia. The number of cases decreased in the 1940's with the development of new plague control techniques and antibiotics. In the last decades, the number of new cases has increased. The current review aimed at reporting some of the epidemiological and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian spotted fever; Rickettsia rickettsii; Amblyomma cajennense.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100002

American visceral Leishmaniasis: a case report
Provedor de dados: 80
Autores: Langoni,H.; Lucheis,S. B.; Da Silva,R. C.; Castro,A. P. B.; Paes,A. C.
Visceral leishmaniasis is a zoonotic disease caused by parasites of the Leishmania genus. Dog is the major source of infection to man, especially in urban areas. The authors report a case of visceral leishmaniasis in a pit bull female dog from Bocaina, São Paulo, Brazil. The animal presented clinical signs compatible with leishmaniasis, including skin lesions in the body and partial damage of the external ears. The indirect fluorescent antibody test (IFAT) demonstrated a titer of 1280, and promastigote forms of Leishmania sp were isolated by the culture of bone marrow puncture. Cytological analysis of the lymph node and smear of the bone marrow puncture revealed macrophages containing amastigote forms of Leishmania sp in their inner region. The test of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Zoonosis; Dog; Diagnosis; Hemoculture; Serology; Polymerase chain reaction (PCR).
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300012

Trypanosomatids in dogs belonging to individuals with chronic Chagas’ disease living in Botucatu town and surrounding region, São Paulo State, Brazil
Provedor de dados: 80
Autores: Lucheis,S. B.; Da Silva,A. V.; Araújo Jr,J. P.; Langoni,H.; Meira,D. A.; Marcondes-Machado,J.
Among domestic animals, dogs are considered to be the major reservoirs of trypanosomatids and, due to their proximity to man, the presence of these parasites in dogs is an alert to actions aiming at triatomine control. Fifty dogs (26 males and 24 females), aged from 2 months to 15 years, belonging to 30 chronic Chagas’ disease individuals from 15 different municipalities in the western region of São Paulo State, Brazil, were subjected to blood collection for the following tests: artificial xenodiagnosis, blood culture, and Polymerase Chain Reaction (PCR). Forty-three (86%) out of 50 dogs were positive to at least one of the tests performed; 34 (68%) were positive to xenodiagnosis, 30 (60%) to blood culture, and 25 (50%) to PCR for T. cruzi and/or T....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; T. rangeli; Dog; Xenodiagnosis; Blood culture; Polymerase chain reaction.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400009

Modified E-test by the addition of EDTA-Tris and dimethyl sulfoxide on the potentiation of the effects of some antimicrobials in Pseudomonas aeruginosa strains isolated from bovine mastitis
Provedor de dados: 45
Autores: Ribeiro,M.G.; Siqueira,A.K.; Langoni,H.; Paes,A.C.; Victória,C.; Silva,A.V. Da; Listoni,F.J.P.; Souza,A.H.
A concentração inibitória mínima-MIC em 30 estirpes de Pseudomonas aeruginosa isoladas de mastite bovina foi avaliada utilizando o E-test padrão e o método modificado, pela adição de Tris-EDTA e DMSO. Os métodos modificados apresentaram redução significativa da MIC das estirpes utilizando a gentamicina, a ciprofloxacina e a norfloxacina.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Mastitis; Pseudomonas aeruginosa; Minimal inhibitory concentration.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000500017

Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanks
Provedor de dados: 45
Autores: Joaquim,S.F.; Guimarães,F.F.; Salina,A.; Junqueira,N.B.; Gomes,E.N.; Langoni,H.
ABSTRACT Mastitis caused by Mycoplasma spp., regardless of species, are considered highly contagious pathogens and, usually was not responsive to antimicrobial therapy. Five dairy herds, comprising 489 animals and 1,956 mammary glands, were used in this study. Milk samples were obtained from bulk tanks and subjected to polymerase chain reaction (PCR) for the identification of Mollicutes, Mycoplasma spp., and Mycoplasma bovis. Moreover, individual samples from cases of clinical and subclinical mastitis in quarters of the dairy herds’ animals that yielded a positive PCR upon bulk tank analysis were subjected to molecular analysis. Only one bulk tank was positive for class Mollicutes by PCR. All positive samples classified as mastitis teats had their DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Milk quality; Polymerase chain reaction; Dairy herds.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601793

Anticorpos para Leptospira spp., Toxoplasma gondii e Neospora caninum em cães errantes albergados em canil privado
Provedor de dados: 45
Autores: Gonçalez,C.C.; Paes,A.C.; Langoni,H.; Da Silva,R.C.; Greca,H.; Camossi,L.G.; Guimarães,F.F.; Ullmann,L.S.
Tipo: Info:eu-repo/semantics/article
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400037

Fatores de virulência em linhagens de Escherichia coli isoladas de mastite bovina
Provedor de dados: 45
Autores: Ribeiro,M.G.; Costa,E.O.; Leite,D.S.; Langoni,H.; Garino Júnior,F.; Victória,C.; Listoni,F.J.P.
Avaliou-se a ocorrência de fatores de virulência e do sorotipo O157:H7 em 120 linhagens de Escherichia coli, isoladas de 80 casos de mastite clínica bovina e 40 de mastite subclínica. Verificou-se alfa-hemolisina em oito (6,7%) linhagens, isoladas de cinco casos de mastite clínica e três de mastite subclínica e em nenhuma das estirpes detectou-se enteroemolisina. A presença de sideróforos foi encontrada em 11 (9,2%) linhagens, sete de mastite clínica e quatro de subclínica. Em duas (1,7%) estirpes isoladas de mastite subclínica, identificou-se enterotoxina STa. Observou-se efeito citopático em células vero compatível com a produção de verotoxina-VT em cinco (4,2%) linhagens, duas de mastite clínica e três subclínicas. Em uma (0,8%) linhagem isolada de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Mastite; Escherichia coli; Virulência; Toxina; Sorotipo O157:H7.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000500004

Efeitos da administração de vitamina E na infecção mamária e na contagem de células somáticas de cabras primíparas desafiadas experimentalmente com Staphylococcus aureus
Provedor de dados: 45
Autores: Paes,P.R.O.; Lopes,S.T.A.; Lopes,R.S.; Kohayagawa,A.; Takahira,R.K.; Langoni,H.
O trabalho teve por objetivo estudar os efeitos da administração de vitamina E sobre a contagem de células somáticas e a infecção da glândula mamária de cabras primíparas desafiadas com a inoculação intramamária de Staphylococcus aureus ao 10º dia pós-parto. Vinte e oito animais foram divididos em quatro grupos, cada um composto por sete cabras primíparas da raça Saanen, como segue: grupo-controle, grupo de animais suplementados com vitamina E, grupo de animais desafiados com S.aureus inoculados na glândula mamária e grupo de animais suplementados com vitamina E e desafiados com S.aureus na glândula mamária. Na segunda e terceira semanas de lactação, a inoculação de S.aureus na glândula mamária permitiu a recuperação do microrganismo no leite e elevou a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caprino; Mastite; Staphylococcus aureus; Contagem de células somáticas; Vitamina E.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000100003

Associação entre a presença de anticorpos anti-Leishmania sp. e anti-Neospora caninum em cães de Bauru, SP
Provedor de dados: 45
Autores: Greca,H.; Silva,A.V.; Langoni,H.
Tipo: Info:eu-repo/semantics/article
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000100032

Atividade funcional neutrofílica em cabras com mastite induzida experimentalmente por Staphylococcus aureus e suplementadas com vitamina E (acetato DL-a-tocoferol)
Provedor de dados: 45
Autores: Lopes,S.T.A.; Paes,P.R.O.; Kohayagawa,A.; Lopes,R.S.; Langoni,H.; Bulla,C.; Langrafe,L.
Avaliou-se a função neutrofílica em cabras com mastite por Staphylococcus aureus, induzida experimentalmente, suplementadas com vitamina E (acetato DL-a-tocoferol). Foram utilizadas 14 cabras gestantes da raça Saanen, com idades entre 8 e 12 meses e com cultura bacteriológica do leite negativa. Sete cabras receberam 2000UI de vitamina E, via intramuscular, no dia do parto e no sétimo dia pós-parto. As outras sete não foram medicadas. No 10º dia pós-parto, os dois grupos foram inoculados com 300 unidades formadoras de colônias de Staphylococcus aureus, cepa ATCC 25923, diluídas em 10ml de solução fisiológica, na glândula mamária esquerda. A função de neutrófilos sangüíneos foi medida pelo teste nitroazul tetrazólio (NBT), antes da inoculação, no momento da...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitamina E; Função de neutrófilo; NBT; Cabras.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000500001

Ocorrência, patógenos e fatores de risco para mastite subclínica em cabras leiteiras
Provedor de dados: 45
Autores: Machado,G.P.; Guimarães,F.F.; Menozzi,B.D.; Salina,A.; Possebon,F.S.; Langoni,H.
ABSTRACT Milk samples from 257 goats and 513 half udders of 10 dairy goat farms were analyzed by microbiological culture, to evaluate the sanitary management, and epidemiological questionnaire results. Multivariate analysis of risk factors for subclinical mastitis in goats, with the adjusted Odds Ratio for subclinical mastitis was performed. The prevalence of subclinical mastitis in goats by microbiological culture was 43.6%, and clinical mastitis was 5.84%. From 178 positive teats by microbiological culture, the most prevalent pathogens were coagulase-negative staphylococci - CNS (70%), S. aureus (13.5%), S. intermedius (7.9%), and Enterobacteriaceae (4%). The risk factors analysis revealed significant association between the disease and the flock size...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mastite caprina; Qualidade do leite; Epidemiologia; Fatores de risco.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501665

Inquérito sorológico para toxoplasmose em equinos na região de Botucatu-SP
Provedor de dados: 45
Autores: Camossi,L.G.; Silva,A.V.; Langoni,H.
Tipo: Info:eu-repo/semantics/article
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000200032

Estudo dos métodos de rotina diagnóstica de mastite no leite de éguas
Provedor de dados: 45
Autores: Motta,R.G.; Ribeiro,M.G.; Langoni,H.; Motta,D.G.; Franco,M.M.J.; Almeida,A.C.S.; Perrotti,I.B.M.; Listoni,F.J.P.; Menozzi,B.D.
Routine diagnosis methods used in bovine mastitis were studied in 55 mares in lactation. The findings of strip cup test, California Mastitis Test-CMT, electronic somatic cell count-CCS, microbiological culture, and in vitro antimicrobial susceptibility profile of isolates were discussed. Streptococcus spp., Staphylococcus spp, and enterobacteria were the most common microorganisms isolated in health and CMT-positive mammary glands. Staphylococcus aureus and Arcanobacterium pyogenes were identified in two mares presenting clinical mastitis. Mean somatic cell count of eight mares without presence of microorganisms in milk was 247.57x10³/mL and 1.621,86x10³/mL in 47 mares with positive microbiological culture. Moderate concordance (63.8%) between positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Égua; Mastite; California Mastitis Test; Contagem de células somáticas.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400034

Abortion and fetal death in bitches due anemia caused by vector-borne diseases
Provedor de dados: 45
Autores: Freire,D.A.C.; Oliveira,I.V.P.M.; Ferreira,H.I.P.; Andrade,V.C.P.; Silva,C.R.F.; Kurissio,J.K.; Ullmann,L.S.; Malossi,C.D.; Joaquim,S.F.; Araújo Júnior,J.P.; Langoni,H.; Calabuig,C.; Megid,J.; Antunes,J.M.A.P.
RESUMO Doenças infecciosas são as maiores responsáveis por falhas reprodutivas (FR) em cadelas, causando aborto, morte fetal e natimortalidade. Este estudo teve como objetivo investigar a associação entre agentes infecciosos, FR inexplicáveis e anemia em cadelas. Todas as amostras maternas e fetais foram negativas para a presença dos principais agentes infecciosos causadores de FR: herpes vírus canino 1, Neospora caninum, Brucella spp. e B. canis, enquanto agentes como o de Leishmania spp., parvovírus canino, Ehrlichia canis e Anaplasma platys foram encontrados em sangue materno. Coinfecções de A. platys/E. canis e A. platys/Leishmania spp. foram diagnosticadas. Os resultados indicam que os animais com anemia causadas por doenças transmitidas por vetores...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Anaplasma; Ehrlichia; Leishmania; Reproductive failures.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501326