Consulta: creatorFacets:"Leão,Evelynne Urzêdo"
Registros recuperados: 2
Data/hora: 11/06/2024 12:49:34

Morphological and molecular characterization of Curvularia lunata pathogenic to andropogon grass
Provedor de dados: 53
Autores: Santos,Patrícia Resplandes Rocha dos; Leão,Evelynne Urzêdo; Aguiar,Raimundo Wagner de Souza; Melo,Maruzanete Pereira de; Santos,Gil Rodrigues dos.
ABSTRACT The fungal genus Curvularia is associated with a number of diseases in plants, commonly producing foliar spots in forage grasses. The objective of this study was to characterize the morphological and molecular diversity of the isolates of Curvularia sp. associated with Andropogon seeds, and to assess both their capacity to transmit disease and the pathogenicity of this fungus to crop. Ten isolates of Curvularia sp. were sourced from Andropogon seeds from agricultural producing regions in the Brazilian states Tocantins and Pará. Morphological characterization was achieved by observing fungus colonies and conidia and molecular characterization by DNA extraction and amplification with sequence-specific primers. The disease transmission was evaluated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Andropogon L.; Curvularia lunata; Diagnosis; Phylogeny; Pathogen.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000200326

Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR
Provedor de dados: 52
Autores: Leão,Evelynne Urzêdo; Spadotti,David Marques de Almeida; Rocha,Kelly Cristina G.; Lima,Élison Fabricio B.; Tavella,Luciana; Turina,Massimo; Krause-Sakate,Renate.
ABSTRACT Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochrome oxidase I gene (mtCOI) were optimized for detection by conventional PCR and real-time PCR. The primers were tested on immature and adult thrips collected from crops and weeds found in São Paulo State. All samples collected were identified as F. schultzei, indicating the high prevalence of this species as vector of tospoviruses in Brazilian fields.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular identification; Real-time PCR; Tospovirus vector.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100454