Consulta: creatorFacets:"Leite,Fábio Pereira Leivas"
Registros recuperados: 8
Data/hora: 15/06/2024 17:16:08

Expressão heteróloga da EMA-2 (equi merozoite antigen) de Theileria equi em Pichia pastoris com potencial utilização em imunobiológicos
Provedor de dados: 65
Autores: Vianna,Ana Muñoz; Gonçales,Relber Aguiar; Lara,Ana Paula de Souza Stori de; Pinto,Luciano da Silva; Nizoli,Leandro Quintana; Leite,Fábio Pereira Leivas.
A piroplasmose equina causada por Theileria equi acomete os equinos de forma endêmica no Brasil e em diversos outros países tropicais e subtropicais. Considerada uma das mais importantes doenças de equinos, causa danos à saúde animal e perdas econômicas. A proteína equi merozoite antigen (EMA-2) é uma das principais proteínas de superfície, expressa nos diversos estágios do ciclo do parasita, estimula resposta imune em animais infectados, tornando-se um possível candidato para utilização em diagnóstico. O gene EMA-2 foi clonado e expresso na levedura Pichia pastoris. A proteína EMA-2 recombinante (rEMA-2) foi caracterizada antigenicamente por Western Blot e por ELISA indireto, utilizando-se soro de equino positivo para theileriose. O resultado do ELISA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equinos; Imunodiagnóstico; Piroplasmose; Theileriose.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014001001830

Adenite equina: sua etiologia, diagnóstico e controle
Provedor de dados: 65
Autores: Moraes,Carina Martins de; Vargas,Agueda Palmira Castagna de; Leite,Fábio Pereira Leivas; Nogueira,Carlos Eduardo Wayne; Turnes,Carlos Gil.
A adenite equina, também conhecida como garrotilho, é uma enfermidade bacteriana contagiosa, causada por Streptococcus equi, subsp. equi, bactéria β hemolítica do grupo C de Lancefield, que afeta o trato respiratório anterior de equinos de todas as idades, com maior prevalência entre um e cinco anos de idade. Caracteriza-se por produzir secreção mucopurulenta das vias aéreas anteriores e linfadenite dos gânglios retrofaríngeos e submandibulares com formação de abscessos. Fatores de virulência de S. equi, subsp. equi, incluem cápsula de ácido hialurônico, hialuronidase, estreptolisina O, estreptoquinase, receptores para Fc de IgG, peptidoglicano e proteína M. Dentre esses fatores, a proteína M tem especial importância por ser de membrana com propriedades...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Garrotilho; Vacina; Streptococcus equi subsp. equi.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000600050

Effect of anaerobic bovine colostrum fermentation on bacteria growth inhibition
Provedor de dados: 65
Autores: Saalfeld,Mara Helena; Pereira,Daniela Isabel Brayer; Valente,Júlia de Souza Silveira; Borchardt,Jéssica Lopes; Weissheimer,Christiano Fanck; Gularte,Márcia Arocha; Leite,Fábio Pereira Leivas.
ABSTRACT: Efficient handling programs that provide high quality colostrum in adequate amounts to dairy farm calves are needed to assure their health and survival. Replacers (or milk substitutes) often become necessary when colostrum presents inadequate quality, or in order to break the cycle of infectious disease transmission. In this study we aimed to assess the effect of anaerobic fermentation processing (colostrum silage) on bacterial that represent interest to animal health. Colostrum samples were inoculated with cultures of Brucella abortus ,  Escherichia coli , Leptospira interrogans serovar Copenhageni , Mycobacterium bovis ,  Salmonella Enteritidis , Salmonella Typhimurium , Staphylococcus aureus , and Bacillus cereus and then subjected to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colostrum silage; Lactic acid bacteria; Calves; Milk substitutes.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202152

Anaerobically fermented colostrum: an alternative for feeding calves
Provedor de dados: 65
Autores: Saalfeld,Mara Helena; Pereira,Daniela Isabel Brayer; Silveira,Kathleen Rodrigues Krüger; Schramm,Renata; Valente,Júlia de Souza Silveira; Borchardt,Jéssica Lopes; Gularte,Márcia Arocha; Leite,Fábio Pereira Leivas.
Milk or commercial milk replacer blends are the most expensive components in final costs of calves breeding. Colostrum is available and it is the appropriate sources for calves' nutrition, being an excellent option as milk substitute. Besides having both nutritional and immunological characteristics that are superior to milk, colostrum represents no costs to the producer. However, difficulties in preservation of colostrum generate controversy results. The aim of this study was to evaluate the anaerobically fermented colostrum (colostrum silage) as liquid diet for dairy calves. We evaluated the microbiological and physicochemical properties of silage, and performance of 31 animals up to 60 days age. From 21 days until 360 days of fermentation we isolated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colostrum silage; Lactobacillus spp.; Calves nutrition.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000900016

Inducers and autoinducers on Salmonella enterica serovar Typhimurium motility, growth and gene expression
Provedor de dados: 65
Autores: Conceição,Rita de Cássia dos Santos da; Sturbelle,Régis Tuchtenhagen; Timm,Cláudio Dias; Leite,Fábio Pereira Leivas.
Genus Salmonella bacteria are among the major pathogenic microorganisms in food. This bacterium pathogenicity is related to a number of virulence factors, among which its flagella. Flagellum expression is one of the virulence factors modulated by Quorum Sensing. Epinephrine produced by mammals uses the same signaling pathway of the 3 bacteria autoinducer. This study evaluated the effect of molecules inducer (epinephrine) and autoinducers (autoinducer 2 and autoinducer 3) and their association with the motility, growth and expression genes flhC, fliA, fliY, motA, motB e fliC of Salmonella Typhimurium (ST). Initially, ST was inoculated in BHI. Then, motility assays, growth curves and gene expression were performed by testing different concentrations of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quorum sensing; Salmonella; Epinephrine; Autoinducer; Motility.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001202201

Brazilian ground beef authentication by multiplex polymerase chain reaction
Provedor de dados: 65
Autores: Oliveira,Andrey Carlos do Sacramento de; Pedroso,Silvia Cristina da Silva; Cardilli,Diogo José; Leite,Fábio Pereira Leivas; Ferreira,Gabrielle Virgínia Lopes; Silva,Andréia Silva da; Roos,Talita Bandeira; Moraes,Carina Martins de; Sousa,Roberta Sales; Monteiro,Roseli do Socorro Dias.
ABSTRACT: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared in triplicate, and dilutions of DNA from Bos taurus and Bubalusbubalis were prepared to determine the detection limit of the method. Concurrently, 91 ground meatsamples sold as “ground beef” were collected from differentstores in northern Brazil andanalyzed bymultiplex PCR. Buffalo DNA was detected in 17.5% of the collected ground meat samples.Our results showed that multiplex PCR is an efficient method for detectingthe incorporation of groundbuffalo meatatpercentages ranging from 10 to 100% and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Species identification; Bos taurus; Bubalusbubalis; DNA; Adulteration.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200652

Staphylococcal slime layers and biofilm from different origins
Provedor de dados: 65
Autores: Cunha,Rodrigo Casquero; Rosa,Michelle Dias Hornes da; Silva,Cleomar da; Santos,Francisco Denis Souza; Leite,Fábio Pereira Leivas.
ABSTRACT:  The genus Staphylococcus comprises some of the most important pathogenic bacteria for both humans and animals. It is responsible for bovine mastitis and canine otitis, besides being present in the microbiota of animals and as a contaminant in food. Its pathogenesis is related to the formation of capsule and biofilm, which contribute to its infectivity. The objective of this study was to observe the production of slime layer and formation of biofilm, which are related to the resistance to antimicrobial agents and presence of icaA and icaD genes, in 41 isolates of Staphylococcus spp. from different origins, provided by the Universidade Federal de Pelotas (UFPEL), Laboratório Regional de Diagnóstico (LRD). Strains of Staphylococcus spp. were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus; Biofilm; Slime layer.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500453

Immunogenicity and cross reactivity indices of Streptococcus equi subsp. equi strains isolated from cases of Strangles and commercial vaccines
Provedor de dados: 65
Autores: Moraes,Carina Martins de; Silva,Mariana Sá e; Vargas,Agueda Palmira Castagna de; Nogueira,Carlos Eduardo Wayne; Leite,Fábio Pereira Leivas; Gil-Turnes,Carlos.
Horse Strangles appears frequently in animals vaccinated with commercial bacterins in Rio Grande do Sul, Brazil. Aiming to know the antigenic relationships of strains recovered from sick animals among them and with two vaccines profusely used in the state, bilateral cross reactivity indices (CRI) were estimated. In addition, the immunogenicity of vaccines prepared with field isolates and commercial vaccines was tested in mice. Antibody titers were measured by ELISA and expressed as seroconversions. Thirteen strains of Streptococcus equi subsp. equi, nine classified biochemically as typical and other four as atypical strains, were recovered from 35 sick horses belonging to 10 herds of Rio Grande do Sul, Brazil. The strains recovered from sick horses showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse Strangles; Streptococcus equi subsp. equi; Cross reactivity indices; Vaccines.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000500024