Consulta: creatorFacets:"Li, Yan"
Registros recuperados: 3
Data/hora: 11/06/2024 07:19:09

Molecular and Immunological Characterization of a Novel 32-kDa Secreted Protein of Babesia microti
Provedor de dados: 19
Autores: Ooka, Hideo; Terkawi, Mohamad A; Cao, Shinuo; Aboge, Gabriel; Goo, Youn-Kyoung; Luo, Yuzi; Li, Yan; Nishikawa, Yoshifumi; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南.
A cDNA encoding the Babesia microti 32-kDa protein was identified by serological immunoscreening of a cDNA expression library and designated as BmP32. The full length of BmP32 contains an open reading frame of 918 base pairs consisting of 306 amino acids having a significant homology with B. microti secreted antigen 1. Antiserum raised against recombinant protein (rBmP32) specifically reacted with a 32-kDa native protein of the parasite lysate using western blot analysis. The indirect immunofluorescent antibody test showed a preferable localization of BmP32 in the cytoplasm of the intra- and extracellular parasites. Moreover, BmP32 was secreted in the cytosol of infected erythrocytes, especially during the peak parasitemia and the recovery phase of the...
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3820

Expression of truncated Babesia gibsoni thrombospondin-related adhesive proteins in Escherichia coli and evaluation of their diagnostic potential by enzyme-linked immunosorbent assay
Provedor de dados: 19
Autores: Sandagdorj, Narantsatsral; Goo, Youn-Kyoung; Terkawi, Mohamad Alaa; Soma, Takehisa; Luo, Yuzi; Li, Yan; Cao, Shinuo; Aboge, Gabriel Oluga; Nishikawa, Yoshifumi; Badgar, Battsetseg; Xuan, Xuenan.
Among the previously established enzyme-linked immunosorbent assays (ELISAs), an ELISA using the full length of recombinant thrombospondin-related adhesive protein of Babesia gibsoni (rBgTRAPf) is considered as the most sensitive diagnostic method for the detection of antibody to B. gibsoni in dogs. However, the expression of rBgTRAPf in high concentration is poor and thus limits its usefulness as diagnostic antigen. To improve its expression level, we have truncated BgTRAPf into two fragments having either N- or C-terminus (BgTRAPn or BgTRAPc). The expression of BgTRAPc protein in Escherichia coli yielded adequate recombinant protein. The specificity and sensitivity of ELISAs with the truncated proteins were determined using B. gibsoni-experimentally...
Palavras-chave: Babesia gibsoni; Diagnosis; Enzyme-linked immunosorbent assay (ELISA); Thrombospondin-related adhesive protein (TRAP).
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3115

Regional Organic Sector Development Planning in China - Case Study in Suichang County of Zhejiang Province
Provedor de dados: 21
Autores: Li, Yan; Li, Gang.
The development of organic sector planning in China has been briefly introduced. Based on the comparison analysis of the advantages and disadvantages for the development of regional organic sector of Shuichang County, the development strategy, priority projects and marketing of organic products of the county have been discussed, so as to workout a demonstrative model for promote regional sustainable development of organic sector.
Tipo: Conference paper, poster, etc.
Palavras-chave: Research methodology and philosophy.
Ano: 2014
URL: http://orgprints.org/23573/7/23573.pdf