Consulta: creatorFacets:"Li,X.L."
Registros recuperados: 4
Data/hora: 02/06/2024 00:35:07

Delivery of the Sox9 gene promotes chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells in an in vitro model
Provedor de dados: 56
Autores: Wang,Z.H.; Li,X.L.; He,X.J.; Wu,B.J.; Xu,M.; Chang,H.M.; Zhang,X.H.; Xing,Z.; Jing,X.H.; Kong,D.M.; Kou,X.H.; Yang,Y.Y.
SRY-related high-mobility-group box 9 (Sox9) gene is a cartilage-specific transcription factor that plays essential roles in chondrocyte differentiation and cartilage formation. The aim of this study was to investigate the feasibility of genetic delivery of Sox9 to enhance chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells (hUC-MSCs). After they were isolated from human umbilical cord blood within 24 h after delivery of neonates, hUC-MSCs were untreated or transfected with a human Sox9-expressing plasmid or an empty vector. The cells were assessed for morphology and chondrogenic differentiation. The isolated cells with a fibroblast-like morphology in monolayer culture were positive for the MSC markers CD44, CD105,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic modification; Tissue engineering; Stem cells; Sox9; Chondrogenesis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400279

Influence of Treg cells and HBV genotype on sustained response and drug resistance in the treatment with nucleoside drugs
Provedor de dados: 56
Autores: Zhang,Y.R.; Li,B.; Wang,C.X.; Zhou,N.; Qi,W.; Li,X.L.; Wu,L.Y.; Wei,S.F.; Zhang,Y.D.
We aimed to investigate the influence of regulatory T cells including CD4+CD25+, CD8+CD28- and hepatitis B virus (HBV) genotype on sustained virological response and tolerance of nucleoside drugs. One hundred and thirty-seven patients were enrolled. Lamivudine was administered to 84 patients. Entecavir was administered to the other 53 patients. Before treatment, biochemical tests, HBV DNA load, HBV serum level, HBV genotype, PB CD3+, CD4+, CD8+, CD4+CD25+/CD3+, and CD8+CD28-/CD3+ frequencies were measured. Based on HBV DNA loads after 4 weeks of therapy, patients were divided into response group and suboptimal response group. The lamivudine group received treatment continuously, and then patients were categorized into non-resistance group and resistance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatitis B chronic; CD4+CD25+; CD8+CD28-; Regulatory T cells; Viral response; Nucleoside.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300606

A three-phase epidemiological study of short and long sleepers in a middle-aged Chinese population: prevalence and characteristics
Provedor de dados: 56
Autores: Hao,Y.L.; Zhang,B.; Jia,F.J.; Li,X.L.; Tang,Y.; Ren,Y.Z.; Liu,W.H.
Epidemiological studies of short and long sleepers have not been conducted previously. We collected socioeconomic, psychological, and polysomnographic characteristics of 6501 parents (3252 men and 3249 women) of 4036 primary school children in Guangzhou city. The study data were collected in three phases. The overall prevalence of short (5 h or less) and long (10 h or more) sleep duration was 0.52 and 0.64%, respectively. Long sleepers had higher Eysenck Personality Questionnaire neuroticism scores [odds ratio (OR)=1.224, 95% confidence interval (CI)=1.047-1.409] and lower education levels (OR=0.740, 95%CI=0.631-0.849) than short sleepers. In the polysomnographic assessment, short, long, and normal sleepers (7-8 h) shared similar durations of Stage 3 sleep...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Short sleeper; Long sleeper; Prevalence; Characteristics; Chinese.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200157

Genetic variations of EBV-LMP1 from nasopharyngeal carcinoma biopsies: potential loss of T cell epitopes
Provedor de dados: 56
Autores: Tang,Y.L.; Lu,J.H.; Cao,L.; Wu,M.H.; Peng,S.P.; Zhou,H.D.; Huang,C.; Yang,Y.X.; Zhou,Y.H.; Chen,Q.; Li,X.L.; Zhou,M.; Li,G.Y.
To find Epstein-Barr virus (EBV) strains with genetic variations of EBV latent membrane protein 1 (EBV-LMP1) from nasopharyngeal carcinoma (NPC), the full-length DNA of LMP1 genes from 21 NPC biopsies obtained in Hunan province in southern China was amplified and sequenced. Our sequences were compared to those previously reported by the Clustal V method. Results showed that all 21 sequences displayed two amino acid changes most frequently in LMP1 of CD4+ T cell epitopes at codons 144 (F®I, 21/21) and 212 (G®S, 19/21) or (G®N, 2/21). We also show that type A EBV strain is prevalent in the cases of NPC from Hunan province with a 30-bp 18/21 deletion, and we highlight that this deletion resulted in loss of one of the CD4+ T cell-restricted epitopes. The other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variation; Epitope; Immune recognition; Latent membrane protein 1; Tumorigenicity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200006