Consulta: creatorFacets:"Lopes,Catalina Romero"
Registros recuperados: 9
Data/hora: 09/06/2024 10:41:25

Genetic diversity in section Rhizomatosae of the genus Arachis (Fabaceae) based on microsatellite markers
Provedor de dados: 74
Autores: Angelici,Carla M.L.C.D.; Hoshino,Andrea Akemi; Nóbile,Paula Macedo; Palmieri,Dario Abel; Valls,José F. Montenegro; Gimenes,Marcos A.; Lopes,Catalina Romero.
The genus Arachis (Fabaceae) native to South America, contains 80 species divided into nine sections, three of which contain species of special economic importance such as the cultivated peanut (Arachis hypogaea), belonging to the section Arachis, and some perennial forage species from sections Caulorrhizae and Rhizomatosae. We used microsatellite markers to assay genetic variability among 77 accessions of four species from section Rhizomatosae, the diploid Arachis burkartii (2n = 2x = 20) and the tetraploid Arachis glabrata, Arachis pseudovillosa and Arachis nitida (2n = 4x = 40). A total of 249 alleles were found in the fifteen loci analyzed and a high degree of intra and interspecific polymorphism was detected. The lowest intraspecific variation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachis; Genetic diversity; Microsatellite; Peanut; Rhizomatosae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100016

Isoenzymatic polymorphism in Citrus spp. and Poncirus trifoliata (L.) Raf. (Rutaceae)
Provedor de dados: 74
Autores: Novelli,Valdenice Moreira; Machado,Marcos Antonio; Lopes,Catalina Romero.
Isoenzymatic polymorphism analysis was used to determine genetic variability among species and hybrids of Citrus spp. and one accession of Poncirus trifoliata (L.) Raf. Ten enzymatic systems aspartate aminotransferase (AAT), acid phosphatase (ACP), leucine aminopeptidase (LAP), 6-phosphogluconate dehydrogenase (6-PGD), isocitrate dehydrogenase (IDH), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), diaphorase (DIA), shikimate dehydrogenase (SKD) and peroxidase (PRX) were analyzed. Twenty loci and 48 alleles were identified. Sweet orange cultivars (C. sinensis (L). Osbeck) showed the highest polymorphism with the largest number of heterozygous loci, although the alleles of those loci were the same in all cultivars, with the exception of Westin and...
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100030

Acid phosphatase polymorphism within and among populations of Cauliflower (Brassica oleracea var botrytis)
Provedor de dados: 74
Autores: Marino,Celso Luis; Gimenes,Marcos Aparecido; Silva,Norberto da; Lopes,Catalina Romero.
Eighty-one lines of cauliflower (Brassica oleracea var. botrytis) from 12 populations used to produce commercial hybrids in Brazil were screened for polymorphism in the acid phosphatase system, in order to evaluate the usefulness of this marker for the determination of the parental contamination level in hybrid seeds. Little polymorphism was detected in the examined lines, but the system appeared to be very useful for hybrid identification, since the only condition required was polymorphism between the two parental lines. If the analyzed lines were used for hybrid production, 8.4% and 12.3% of the possible crosses would result in hybrids which can be positively identified using the APS-1 and B1 loci, respectively. If only one plant of each homozygous type...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isoenzyme; Polymorphism; Cauliflower.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100015

Heterologous microsatellite primer pairs informative for the whole genus Arachis
Provedor de dados: 74
Autores: Hoshino,Andrea Akemi; Bravo,Juliana Pereira; Angelici,Carla M.L.C.D.; Barbosa,Andréa V. Gobbi; Lopes,Catalina Romero; Gimenes,Marcos A.
The genus Arachis currently comprises 69 described species, some of which have potential and real value as human and animal foods. These Arachis species have been collected and maintained in germplasm banks to provide material that can be used as sources of genes in breeding programs and for the selection of new cultivars. One of the principal objectives of germplasm conservation is the evaluation of genetic variability, which is best conducted using molecular markers. We investigated the use of heterologous primers to amplify microsatellite loci that could be used to evaluate genetic variability in Arachis germplasm. Fifteen microsatellite primer pairs were tested in 76 accessions of 34 species from the nine Arachis sections. The data indicated that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peanut; Genetic relationship; Molecular markers; Wild relatives.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400016

Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis
Provedor de dados: 74
Autores: Bravo,Juliana Pereira; Hoshino,Andrea Akemi; Angelici,Carla Maria Lara C.D.; Lopes,Catalina Romero; Gimenes,Marcos Aparecido.
The Arachis section is the most important of the nine sections of the genus Arachis because it includes the cultivated peanut, Arachis hypogaea. The genetic improvement of A. hypogaea using wild relatives is at an early stage of development in spite of their potential as sources of genes, including those for disease and pests resistance, that are not found in the A. hypogaea primary gene pool. Section Arachis species germplasm has been collected and maintained in gene banks and its use and effective conservation depends on our knowledge of the genetic variability contained in this material. Microsatellites are routinely used for the analysis of genetic variability because they are highly polymorphic and codominant. The objective of this study was to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Germplasm; Peanut; Transferability; Wild species.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300021

Isoenzymatic variation in the germplasm of Brazilian races of maize (Zea mays L.)
Provedor de dados: 74
Autores: Gimenes,Marcos Aparecido; Lopes,Catalina Romero.
There are more than 200 races of maize (Zea mays L.) divided into three groups (ancient commercial races, the recent commercial races, and indigenous races). Although the indigenous races have no commercial value, they have many important characteristics which can be incorporated into maize breeding programs. Most Brazilian indigenous germplasm race stocks were collected at least 40 years ago, and nothing is known of the genetic variability present in this germplasm. The genetic variability was assayed in 15 populations from four indigenous races of maize (Caingang, Entrelaçado, Lenha and Moroti) and five indigenous cultivars, using five isoenzymatic systems encoded by 14 loci. The analysis revealed a low level of variability among the samples studied....
Tipo: Info:eu-repo/semantics/article
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200023

Avaliação da variabilidade isoenzimática de cinco cultivares de amendoim
Provedor de dados: 53
Autores: Galgaro,Maria Leticia; Lopes,Catalina Romero.
A variabilidade genética foi avaliada dentro e entre amostras de diferentes cultivares de amendoim, Arachis hypogaea L., conhecidos como Roxo, Tatu Branco, Tatu Vermelho, Tatuí Vermelho e Tatuí (sementes com película branca), fornecidos por fazendas situadas nas regiões dos municípios de Marília, Presidente Prudente e São Manuel. Para tal análise, foi utilizada a técnica de eletroforese horizontal em gel de poliacrilamida, para os sistemas da leucil-aminopeptidase (LAP), aspartato aminotransferase (ATT) e peroxidase (PER). No sistema da leucil-aminopeptidase, foram observadas três bandas enzimáticas, denominadas LAP-A, LAP-B e LAP-C. Os padrões de bandas obtidos para o sistema da aspartato-aminotransferase mostraram a existência de três bandas anódicas,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eletroforese; Isoenzimas; Cultivares de amendoim; Variabilidade genética.
Ano: 1994
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87051994000200002

Análise cromossômica e molecular do javali europeu Sus scrofa scrofa e do suíno doméstico Sus scrofa domesticus
Provedor de dados: 62
Autores: Gimenez,Danilo Lucano; Mota,Lígia Souza Lima Silveira da; Curi,Rogério Abdallah; Rosa,Guilherme Jordão de Magalhães; Gimenes,Marcos Aparecido; Lopes,Catalina Romero; Lucca,Edmundo José de.
A presente investigação teve como objetivos: analisar animais presentes em diferentes criações de javalis no estado de São Paulo, com o intuito de auxiliar a identificação de javalis "puros" assim como javalis híbridos provenientes do cruzamento com o suíno doméstico, para tanto foram utilizadas avaliação do fenótipo dos animais, análises citogenéticas e da técnica molecular de RAPD (Random Amplified Polymorphic DNA).O estudo do número de cromossomos nas células diplóides em 104 animais destinados a análise citogenética e fenotípica, revelou polimorfismo de 2n=36, 37 e 38 cromossomos. Por meio da técnica de bandamento GTG foi possível identificação da translocação Robertsoniana entre os cromossomos 15 e 17 como responsável por esse polimorfismo. Todavia,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Javali; Híbrido; Citogenética; RAPD; Fenótipos.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000200009

Evaluation of nine microsatellite loci and misidentification paternity frequency in a population of Gyr breed bovines
Provedor de dados: 62
Autores: Curi,Rogério Abdallah; Lopes,Catalina Romero.
Paternity misidentification is harmful due to the reduction in annual genetic earnings of the population and because it endangers an efficient genetic improvement program. The objectives of the present study was to evaluate nine microsatellites in Paternity Testing and to investigate misidentification paternity frequency in families of Gyr breed bovines population. In the present experiment blood samples from forty Gir breed families ( bull / cow / calf ), registered pure breed in the Zebu Breeders Brazilian Association (ABCZ) were used. The most part of the microsatellites used in this work were recommended by the International Society of Animal Genetics (ISAG). The genomic DNA extraction was performed from whole blood samples. The microsatellites...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Microsatellites; Paternity; Bovine; Gyr.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000300004