Consulta: creatorFacets:"Lopes,Francisco Luiz Gumes"
Registros recuperados: 4
Data/hora: 08/06/2024 11:38:43

Concentration by membrane separation processes of a medicinal product obtained from pineapple pulp
Provedor de dados: 52
Autores: Lopes,Francisco Luiz Gumes; Severo Júnior,João Baptista; Souza,Roberto Rodrigues de; Ehrhardt,Daniela Diniz; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
The concentration of pineapple juice is needed to retain the bromelain activity and to standardize the composition and proteolytic activity. Thus, this work aimed to obtain a pure bromelain extract from the Ananas comosus L. Merril juice by membrane separation process. A 2² experimental planning was used to study the influence of pH and transmembrane pressure on the activity recovery by micro-filtration using a plain membrane. In second step, this enzyme was purified by the ultra-filtration using a 10 kDa millipore kit. The best operation condition to bromelain concentration using the plain membrane was at pH 7.5 and transmembrane pressure of 0.05 bar, while 85% of bromelain activity was recovered. Ultra-filtration retained 100% of proteolytic activity and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas comosus; Bromelain; Membrane separation processes; Purification; Medicinal product.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200024

Response surface methodology to evaluation the recovery of amylases by hollow fiber membrane
Provedor de dados: 52
Autores: Severo Júnior,João Baptista; Oliveira,Laura Sampaio de Sá; Sardeiro,Fernanda Silva; Souza,Roberto Rodrigues de; Lopes,Francisco Luiz Gumes; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
This work aimed to study the pH and the transmembrane pressure effects during the recovery of alpha and beta amylases enzymes from corn malt (Zea mays) by hollow fiber membrane. The optimal condition was obtained for a statistical model, established by response surface methodology (RSM). The response surface analysis showed that the best operation condition for amylolitics enzymes recovery by hollow fiber membrane was 0.05 bar and pH 5.00, while the enzymes were purified about of 26 times.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hollow fiber membrane; Corn malt; Alpha and beta amylase; RSM.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400017

Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation
Provedor de dados: 52
Autores: Leite,Nadjma Souza; Lima,Aloísio André Bomfim de; Santana,José Carlos Curvelo; Lopes,Francisco Luiz Gumes; Lédo,Ana da Silva; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de.
This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enzymatic activity; Bromelain; Pérola pineapple; Imperial pineapple; Production in vitro.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500002

Viability in the production of a drug extracted from Ananas comosus by a flat membrane system
Provedor de dados: 52
Autores: Lopes,Francisco Luiz Gumes; Sbruzzi,Dalva; Barros,Kleber Vanio Gomes; Ferreira,Juliana Ferrari; Santana,José Carlos Curvelo; Souza,Roberto Rodrigues de; Tambourgi,Elias Basile.
The aim of this work was to study the production of e bromelain from the Ananas comosus L. Merril, by determining the process conditions using flat membranes. The production system modeling generated a hyperbolical curve and the optimization by response surfaces showed an influence of the transmembrane pressure higher than the pH influence. The cost of the production of bromelain from A. comosus was estimated 9 to 13 times lower than Sigma's retail sales price and 6.5 to 8.5 times lower than when this enzyme was obtained through a liquid-liquid extraction, which showed the economical feasibility of the process.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas comosus; Bromelain; Flat membrane; Production cost; Optimization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300018