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Identification of functionally important acidic residues in transducin by group-specific labeling
Provedor de dados: 48
Autores: KOSOY,ANA; MÖLLER,CAROLINA; PERDOMO,DEISY.
Transducin (T), a GTP-binding protein involved in phototransduction of rod photoreceptor cells, is a heterotrimer arranged as two units, the a-subunit (Ta) and the bg-complex (Tbg). The role of the carboxyl groups in T was evaluated by labeling with N, N'-dicyclohexylcarbodiimide (DCCD) and 1-ethyl 3-(3-dimethylaminopropyl) carbodiimide (EDC). Only a minor effect on the binding of b, g-imido guanosine 5'-triphosphate (GMPpNp) to T was observed in the presence of the hydrophobic carbodiimide, DCCD. Similarly, the GMPpNp binding activity of the reconstituted holoenzyme was not significantly affected when Ta was combined with DCCD-treated Tbg. However, the binding of guanine nucleotides to the reconstituted T was ~50% inhibited when DCCD-labeled Ta was...
Tipo: Journal article
Palavras-chave: Chemical modification; G-protein-coupled signaling; Group-specific labeling; Transducin; Visual process.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300010