Consulta: creatorFacets:"Machado,O.L.T."
Registros recuperados: 5
Data/hora: 13/06/2024 15:32:57

PHOSPHOLIPASE A2 FROM Lachesis muta (BUSHMASTER) SNAKE VENOM:BIOCHEMICAL CHARACTERIZATION AND EFFECTS ON PLATELET AGGREGATION
Provedor de dados: 79
Autores: Fuly,A.L.; Machado,O.L.T.; Alves,E.W.; Carlini,C.R.
Tipo: Info:eu-repo/semantics/other
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000200023

Isolation and intracellular localization of insulin-like proteins from leaves of Bauhinia variegata
Provedor de dados: 56
Autores: Azevedo,C.R.; Maciel,F.M.; Silva,L.B.; Ferreira,A.T.S.; da Cunha,M.; Machado,O.L.T.; Fernandes,K.V.S.; Oliveira,A.E.A.; Xavier-Filho,J.
Evidence based on immunological cross-reactivity and anti-diabetic properties has suggested the presence of insulin-like peptides in plants. The objective of the present study was to investigate the presence of insulin-like proteins in the leaves of Bauhinia variegata ("pata-de-vaca", "mororó"), a plant widely utilized in popular medicine as an anti-diabetic agent. We show that an insulin-like protein was present in the leaves of this plant. A chloroplast protein with a molecular mass similar to that of bovine insulin was extracted from 2-mm thick 15% SDS-PAGE gels and fractionated with a 2 x 24 cm Sephadex G-50 column. The activity of this insulin-like protein (0.48 mg/mL) on serum glucose levels of four-week-old Swiss albino (CF1) diabetic mice was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bauhinia variegata; Plant insulin; Chloroplast; Medicinal plant.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100007

Lima bean (Phaseolus lunatus) seed coat phaseolin is detrimental to the cowpea weevil (Callosobruchus maculatus)
Provedor de dados: 56
Autores: Moraes,R.A.; Sales,M.P.; Pinto,M.S.P.; Silva,L.B.; Oliveira,A.E.A.; Machado,O.L.T.; Fernandes,K.V.S.; Xavier-Filho,J.
The presence of phaseolin (a vicilin-like 7S storage globulin) peptides in the seed coat of the legume Phaseolus lunatus L. (lima bean) was demonstrated by N-terminal amino acid sequencing. Utilizing an artificial seed system assay we showed that phaseolin, isolated from both cotyledon and testa tissues of P. lunatus, is detrimental to the nonhost bruchid Callosobruchus maculatus (F) (cowpea weevil) with ED50 of 1.7 and 3.5%, respectively. The level of phaseolin in the seed coat (16.7%) was found to be sufficient to deter larval development of this bruchid. The expression of a C. maculatus-detrimental protein in the testa of nonhost seeds suggests that the protein may have played a significant role in the evolutionary adaptation of bruchids to legume seeds.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lima bean; Phaseolin (vicilin); 7S storage globulin; Seed coat; Cowpea weevil; Resistance to bruchids.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200005

A protein with amino acid sequence homology to bovine insulin is present in the legume Vigna unguiculata (cowpea)
Provedor de dados: 56
Autores: Venâncio,T.M.; Oliveira,A.E.A.; Silva,L.B.; Machado,O.L.T.; Fernandes,K.V.S.; Xavier-Filho,J.
Since the discovery of bovine insulin in plants, much effort has been devoted to the characterization of these proteins and elucidation of their functions. We report here the isolation of a protein with similar molecular mass and same amino acid sequence to bovine insulin from developing fruits of cowpea (Vigna unguiculata) genotype Epace 10. Insulin was measured by ELISA using an anti-human insulin antibody and was detected both in empty pods and seed coats but not in the embryo. The highest concentrations (about 0.5 ng/µg of protein) of the protein were detected in seed coats at 16 and 18 days after pollination, and the values were 1.6 to 4.0 times higher than those found for isolated pods tested on any day. N-terminal amino acid sequencing of insulin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; Insulin-like; Cowpea; Vigna unguiculata; Developing fruits; Sequence homology; Evolution.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900004

Simultaneous allergen inactivation and detoxification of castor bean cake by treatment with calcium compounds
Provedor de dados: 56
Autores: Fernandes,K.V.; Deus-de-Oliveira,N.; Godoy,M.G.; Guimarães,Z.A.S.; Nascimento,V.V.; Melo,E.J.T. de; Freire,D.M.G.; Dansa-Petretski,M.; Machado,O.L.T.
Ricinus communis L. is of great economic importance due to the oil extracted from its seeds. Castor oil has been used for pharmaceutical and industrial applications, as a lubricant or coating agent, as a component of plastic products, as a fungicide or in the synthesis of biodiesel fuels. After oil extraction, a castor cake with a large amount of protein is obtained. However, this by-product cannot be used as animal feed due to the presence of toxic (ricin) and allergenic (2S albumin) proteins. Here, we propose two processes for detoxification and allergen inactivation of the castor cake. In addition, we establish a biological test to detect ricin and validate these detoxification processes. In this test, Vero cells were treated with ricin, and cell death...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ricinus communis; 2S albumin; Ricin; Biodiesel fuel; Solid-state fermentation; Vero cell.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100002