Consulta: creatorFacets:"Magalhães,Francisco de Araújo"
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Data/hora: 08/06/2024 18:12:08

Anticoagulant activity of a sulfated polysaccharide isolated from the green seaweed Caulerpa cupressoides
Provedor de dados: 52
Autores: Rodrigues,José Ariévilo Gurgel; Vanderlei,Edfranck de Sousa Oliveira; Bessa,Érika Façanha; Magalhães,Francisco de Araújo; Paula,Regina Célia Monteiro de; Lima,Vilma; Benevides,Norma Maria Barros.
The aim of this study was to evaluate certain molecular characteristics of a sulfated polysaccharide (SPs) with anticoagulant properties, isolated from Caulerpa cupressoides (Chlorophyta). Crude SPs were extracted by proteolytic digestion (papain), followed by ion-exchange chromatography on a DEAE-cellulose column. The fractions obtained were analyzed for molecular mass, 0.5% agarose gel electrophoresis and chemical composition. The activated partial thromboplastin time (APTT) test was applied using normal human plasma and standard heparin (HEP) (193 IU mg-1). The yield was ~ 3%, and the chromatography procedure separated the material into three different SP fractions (F I, F II and F III), eluted at the concentrations of 0.50, 0.75 and 1.00 M of NaCl,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine algae; Sulfated macromolecules; Chemical analysis; APTT test.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400007