Consulta: creatorFacets:"Mahmood,Tariq"
Registros recuperados: 5
Data/hora: 01/06/2024 01:03:55

Multiple pulses improve electroporation efficiency in Agrobacterium tumefaciens
Provedor de dados: 69
Autores: Mahmood,Tariq; Zar,Tamkina; Saqlan Naqvi,S.M.
Electroporation entails brief, high intensity pulse to create transient pores in the cell membrane to facilitate the entry of exogenous macromolecules, which may otherwise be excluded. Removal of the external field leads to the resealing of the membrane electropores permitting the survival of the electrically stimulated recipient cells. Using this technique foreign deoxyribonucleic acid (DNA) has been successfully introduced into many cell types both from prokaryotes and eukaryotes. Increase in pulse voltage and length beyond a critical limit has been reported to decrease transformation efficiency, hence in this study we have investigated another strategy i.e. increase in the number of pulses at constant high voltage and pulse duration. Commonly used...
Tipo: Journal article
Palavras-chave: Agrobacterium; Electroporation; Pulse number; Transformation efficiency.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100015

Molecular Characterization of a MYB Protein from Oryza sativa for its Role in Abiotic Stress Tolerance
Provedor de dados: 52
Autores: Deeba,Farah; Sultana,Tasawar; Javaid,Bushra; Mahmood,Tariq; Naqvi,S. M. S.
ABSTRACT The MYB family represents one of the most abundant classes of transcriptional regulators that perform pivotal role under different developmental processes and abiotic stresses. In present study, a MYB gene from Oryza sativa was selected for functional characterization. Bioinformatics analysis revealed that OsMYB1 cDNA encodes R2-R3 type DNA binding domain consisting of 413 amino acids having size of 44 kDa and pI of 6.24. DNA binding domain containing region was cloned and over-expressed in E. coli. Then, the survival of pGEX-OsMYB1 transformed E. coli cells was compared with control plasmid under different concentrations of NaCl, mannitol, high and low temperature. pGEX-OsMYB1 enhanced the survival of cells at high temperature and salinity....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic stress; E. coli; Oryza sativa; OsMYB1; PGEX; Real time qPCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100411

Fabrication and Characterization of Gliclazide Loaded Microcapsules
Provedor de dados: 52
Autores: Asad,Muhammad; Bashir,Sajid; Mahmood,Tariq; Nazir,Imran; Imran,Muhammad; Karim,Sabiha; ul Hassnain,Fakhar.
This study aimed to formulate, characterize and evaluate the Gliclazide (GLZ) microcapsules prepared with sodium alginate, guar gum and pectin in different ratios by ionotropic-gelation method. The microcapsules were evaluated against different parameters such as particle size and shape, Carr's index, Hausner's ratio, rheological studies and drug release kinetics. Fourier Transform Infra Red (FTIR) and Differential Scanning Calorimetric (DSC) studies demonstrated the absence of any drug - polymers interaction. Promising characteristics were observed in rheological behavior and release kinetics. The size of microcapsules and percentage yield was in the range of 676 to 727 µm and 69 to 77%, respectively. Scanning electron micrographs revealed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microcapsules; Gliclazide; Ionotropic-gelation method; Guar gum; Pectin.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600874

Silymarin-Laden PVP-Nanocontainers Prepared Via the Electrospraying Technique for Improved Aqueous Solubility and Dissolution Rate
Provedor de dados: 52
Autores: Yousaf,Abid Mehmood; Malik,Usman Rashid; Shahzad,Yasser; Hussain,Talib; Khan,Ikram Ullah; Din,Fakhar Ud; Mahmood,Tariq; Ahsan,Hafiz Muhammad; Syed,Ahmed Shah; Akram,Muhammad Rouf.
Abstract The aim of the present research was to develop a silymarin-laden PVP-nanocontainer providing ameliorated aqueous solubility and dissolution of the drug. Several silymarin-laden formulations were formed with varying quantities of PVP and SDS via the solvent evaporation method using the electrospraying technique. The influence of the hydrophilic carriers on solubility and dissolution was explored. The solid-state characterization was carried out by particle-size analysis, PXRD, DSC, FTIR and SEM. All of the formulations demonstrated better solubility and dissolution than did silymarin plain powder. Both the SDS and PVP had positive effects on solubility and dissolution of silymarin in the aqueous media. An increased solubility was attained as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silymarin; Electrospraying; PVP-nanocontainers; Aqueous solubility; Dissolution.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100612

Fabrication, physicochemical characterization and preliminary efficacy evaluation of a W/O/W multiple emulsion loaded with 5% green tea extract
Provedor de dados: 60
Autores: Mahmood,Tariq; Akhtar,Naveed; Khan,Barkat Ali; Rasul,Akhtar; Khan,Haji M. Shoaib.
Complex multiple emulsions have an excellent ability to fill large volumes of functional cosmetic agents. This study was aimed to encapsulate large volume of green tea in classical multiple emulsion and to compare its stability with a multiple emulsion without green tea extract. Multiple emulsions were developed using Cetyl dimethicone copolyol as lipophilic emulsifier and classic polysorbate-80 as hydrophilic emulsifier. Multiple emulsions were evaluated for various physicochemical aspects like conductivity, pH, microscopic analysis, rheology and these characteristics were followed for a period of 30 days in different storage conditions. In vitro and in vivo skin protection tests were also performed for both kinds of multiple emulsions i.e. with active...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cosmetics; Green tea; Multiple emulsion; Rheology; Skin; Stability.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200016