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Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
Provedor de dados: 5
Autores: Joubert, Caroline; Piquemal, David; Marie, Benjamin; Manchon, Laurent; Pierrat, Fabien; Zanella-cleon, Isabelle; Cochennec-laureau, Nathalie; Gueguen, Yannick; Montagnani, Caroline.
Background: The shell of the pearl-producing bivalve Pinctada margaritifera is composed of an organic cell-free matrix that plays a key role in the dynamic process of biologically-controlled biomineralization. In order to increase genomic resources and identify shell matrix proteins implicated in biomineralization in P. margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell. Results: We report the functional analysis of 276 738 sequences, leading to the constitution of an unprecedented catalog of 82 P. margaritifera biomineralization-related mantle protein sequences. Components of the current "chitin-silk fibroin gel-acidic macromolecule" model of...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00020/13154/10202.pdf