Consulta: creatorFacets:"Mantovani,Mário Sérgio"
Registros recuperados: 13
Data/hora: 06/06/2024 09:35:30

Assessment of two medicinal plants, Psidium guajava L. and Achillea millefolium L., in in vitro and in vivo assays
Provedor de dados: 74
Autores: Teixeira,Rosangela de Oliveira; Camparoto,Marjori Leiva; Mantovani,Mário Sérgio; Vicentini,Veronica Elisa Pimenta.
The use of medicinal plants by the general population is an old and still widespread practice, which makes studies of their genotoxicity essential. Psidium guajava L. and Achillea millefolium L. are examples of plants commonly used in popular medicine. P. guajava L. is indicated for diarrhea and also as an antiseptic, while A. millefolium L. is indicated as an analgesic, antispasmodic, digestive, diuretic, antiseptic, astringent, emollient, wound healer and hemorrhoid medication. The aim of this study was to determine the effects of the infusions of these two plant species on chromosomes and the cell cycle. Leaves from the plants were used to prepare infusions, in the same manner as teas, but at two different concentrations. Allium cepa L. root-tip cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal herbs; Chromosome damage; Mutagenicity test.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400021

Effects of Maytenus ilicifolia Mart. and Bauhinia candicans Benth infusions on onion root-tip and rat bone-marrow cells
Provedor de dados: 74
Autores: Camparoto,Marjori Leiva; Teixeira,Rosangela de Oliveira; Mantovani,Mário Sérgio; Vicentini,Veronica Elisa Pimenta.
Medicinal plants are widely used to treat various diseases, and in Brazil the plants Maytenus ilicifolia Mart. and Bauhinia candicans Benth are commonly used in popular medicine. However, there are a large number of compounds in plants which can produce alterations in genetic material, and this study was conducted to investigate any possible mutagenic and cytotoxic effects that M. ilicifolia and B. candicans infusions may have on the cell cycle and chromosomes. Infusions were prepared with in natura leaves to give two concentrations of infusions, one at the concentration normally used by the population in general and the other at 10 times this value (i.e. 3.5 and 35 mg/mL for M. ilicifolia and 0.465 and 4.65 mg/mL for B. candicans). Onion (Allium cepa L.)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa test; Bauhinia candicans; Chromosome damage; Maytenus ilicifolia; Medicinal herbs; Mutagenicity test; Wistar rat bone marrow cells.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100016

Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips
Provedor de dados: 74
Autores: Matsumoto,Silvia Tamie; Mantovani,Mário Sérgio; Malaguttii,Mirtis Irene Ariza; Dias,Ana Lúcia; Fonseca,Inês Cristina; Marin-Morales,Maria Aparecida.
Cytotoxicity of metals is important because some metals are potential mutagens able to induce tumors in humans and experimental animals. Chromium can damage DNA in several ways, including DNA double strand breaks (DSBs) which generate chromosomal aberrations, micronucleus formation, sister chromatid exchange, formation of DNA adducts and alterations in DNA replication and transcription. In our study, water samples from three sites in the Córrego dos Bagres stream in the Franca municipality of the Brazilian state of São Paulo were subjected to the comet assay and micronucleus test using erythrocytes from the fish Oreochromis niloticus. Nuclear abnormalities of the erythrocytes included blebbed, notched and lobed nuclei, probably due to genotoxic chromium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Chromium; Chromosomes aberrations; Comet assay; Micronucleus; Oreochromis niloticus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100028

Determination of the antimutagenicity of an aqueous extract of Rhizophora mangle L. (Rhizophoraceae), using in vivo and in vitro test systems
Provedor de dados: 74
Autores: Malini,Maressa; Marin-Morales,Maria Aparecida; Mantovani,Mário Sérgio; Jamal,Claudia Masrouah; Nati,Natália; Passos,Tatiane da Silva; Matsumoto,Silvia Tamie.
An aqueous extract of Rhizophora mangle L. bark is used as raw material in pottery making in the State of Espirito Santo, Brazil. This extract presents large quantities of tannins, compounds possessing antioxidant properties. Tannin antioxidant activity, as a plant chemical defense mechanism in the process of stabilizing free radicals, has been an incentive to studies on anti-mutagenicity. The present work aimed to evaluate possible antimutagenic activity of a R. mangle aqueous extract, using the Allium cepa test-system and micronuclear (MN) assay with blockage of cytokinesis in Chinese hamster ovary cells (CHO-K1). The Allium cepa test-system indicated antimutagenic activity against the damage induced by the mutagenic agent methyl methanesulfonate. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhizophora mangle; Antimutagenicity; Allium cepa; CHO-K1.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100029

LNO3 AND L3 Are Associated With Antiproliferative And Pro-Apoptotic Action In Hepatoma Cells
Provedor de dados: 74
Autores: Zanelatto,Leonardo Campos; Silva,Patrícia Benites Gonçalves da; Sartori,Daniele; Panis,Carolina; Lepri,Sandra; Fátima,Ângelo de; Mantovani,Mário Sérgio.
Abstract The identification of antitumoral substances is the focus of intense biomedical research. Two structural analogues of thalidomide, LNO3 and L3, are two synthetic compounds that might possess such antitumor properties. We evaluated the toxicological effects of these substances, including cytotoxicity, genotoxicity and induction of apoptosis in HTC cells. Additionally, the production of free radicals (nitric oxide and superoxide) was investigated, and the expression of caspases genes 3, 8, and 9 were determined by RT-qPCR. The compounds exhibited cytotoxic effects that resulted in inhibited cell proliferation. LNO3 showed to be more effective and toxic than L3 in all assays. LNO3 stimulated the release of NO and superoxide, which was accompanied by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thalidomide analogues; Antiproliferative activity; Cytotoxicity; Free radicals; Apoptosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200270

In vivo evaluation of the antimutagenic and antigenotoxic effects of β-glucan extracted from Saccharomyces cerevisiae in acute treatment with multiple doses
Provedor de dados: 74
Autores: Oliveira,Rodrigo Juliano; Salles,Maria José Sparça; Silva,Ariane Fernanda da; Kanno,Tatiane Yumi Nakamura; Lourenço,Ana Carolina dos Santos; Leite,Véssia da Silva; Matiazi,Hevenilton José; Pesarini,João Renato; Ribeiro,Lúcia Regina; Mantovani,Mário Sérgio.
Ample evidence suggests that cancer is triggered by mutagenic damage and diets or supplements capable of reducing such incidences can be related to the prevention of neoplasy development or to an improvement in life quality of patients who undergo chemotherapy. This research aimed to evaluate the antimutagenic and antigenotoxic activity of β-glucan. We set up 8 experimental groups: control (Group 1), cyclophosphamide (Group 2), Groups 3-5 to assess the effect of β-glucan administration, and Groups 6-8 to evaluate the association between cyclophosphamide and β-glucan. The intraperitonial concentrations of β-glucan used were 100, 150 and 200 mg/kg. Micronucleus and comet assays showed that within the first week of treatment β-glucan presented a damage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-glucan; Cyclophosphamide; Antimutagenicity; Antigenotoxicity; Mice.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300017

Genotoxic evaluation of an industrial effluent from an oil refinery using plant and animal bioassays
Provedor de dados: 74
Autores: Rodrigues,Fernando Postalli; Angeli,José Pedro Friedmann; Mantovani,Mário Sérgio; Guedes,Carmen Luisa Barbosa; Jordão,Berenice Quinzani.
Polycyclic aromatic hydrocarbons (PAHs) are genotoxic chemicals commonly found in effluents from oil refineries. Bioassays using plants and cells cultures can be employed for assessing environmental safety and potential genotoxicity. In this study, the genotoxic potential of an oil refinery effluent was analyzed by means of micronucleus (MN) testing of Alium cepa, which revealed no effect after 24 h of treatment. On the other hand, primary lesions in the DNA of rat (Rattus norvegicus) hepatoma cells (HTC) were observed through comet assaying after only 2 h of exposure. On considering the capacity to detect DNA damage of a different nature and of these cells to metabolize xenobiotics, we suggest the association of the two bioassays with these cell types,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; HTC cells; Micronucleus; Comet assay; Petroleum waste disposal.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100028

Comet assay comparison of different Corbicula fluminea (Mollusca) tissues for the detection of genotoxicity
Provedor de dados: 74
Autores: Rigonato,Janaina; Mantovani,Mário Sérgio; Jordão,Berenice Quinzani.
The comet assay was used to study the sensitivity of the widely distributed freshwater bivalve mollusk Corbicula fluminea to the DNA-damaging alkylating-agent methylmethane sulfonate (MMS). This study was undertaken to ascertain if C. fluminea is a good bioindicator of pollutants in aquatic environments and identify which C. fluminea tissue is most effective and practical for genotoxicity studies. The mollusks were exposed to 0.6, 1.2 or 2.4 X 10-4 M MMS for 40 min and their hemolymph, gill tissue and digestive gland tissue assessed for the level of DNA damage and the time needed for the tissues to recovery. Regression analysis showed a direct linear dose-response relationship between MMS concentration and the number of damaged cells for hemolymph and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corbicula fluminea; Hemolymph; Gills; Digestive gland; Comet assay; Biomonitoring.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300023

Evaluation of extracts from Coccoloba mollis using the Salmonella/microsome system and in vivo tests
Provedor de dados: 74
Autores: Tsuboy,Marcela Stefanini; Marcarini,Juliana Cristina; Ferreira,Dalva Trevisan; Ferraz,Elisa Raquel Anastácio; Chequer,Farah Maria Drumond; Oliveira,Danielle Palma de; Ribeiro,Lúcia Regina; Mantovani,Mário Sérgio.
The common everyday use of medicinal plants is an ancient, and still very widespread practice, whereby the need for studies on their possible toxicity and mutagenic properties. The species Coccoloba mollis has been much used in phytotherapy, mainly in cases involving loss of memory and stress. In order to investigate its genotoxic and mutagenic potential, ethanolic extracts from the leaves and roots underwent Salmonella/microsome assaying (TA98 and TA100 strains, with and without exogenous metabolism - S9), besides comet and micronucleus tests in vivo.There was no significant increase in the number of revertants/plate of Salmonella strains in any of the analyzed root-extract concentrations, although the extract itself was extremely toxic to the Salmonella...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Coccoloba mollis; Ames test; In vivo comet assay; In vivo micronucleus assay; Polygonaceae.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300027

Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays
Provedor de dados: 74
Autores: Bellini,Marilanda Ferreira; Cabrioti,Leonardo Neves; Terezan,Ana Paula; Jordão,Berenice Quinzani; Ribeiro,Lúcia Regina; Mantovani,Mário Sérgio.
Functional food investigations have demonstrated the presence of substances that could be beneficial to human health when consumed. However, the toxic effects of some substances contained in foods have been determined. Reported medicinal and nutritive properties have led to the extensive commercialization of the basidiomycete fungi Agaricus blazei Murrill (sensu Heinemann), also known as Agaricus brasiliensis Wasser et al., Agaricus subrufescens Peck or the Brazilian medical mushroom (BMM). Different methanolic extract fractions (ME) of this mushroom were submitted to the cytokinesis-block micronucleus (CBMN) clastogenic assay and the hypoxanthine-guanine phosphoribosyl transferase locus (HGPRT) assay for gene mutation, both using Chinese hamster ovary...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus blazei; Hgprt locus; Micronucleus.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100021

Effects of β-glucan polysaccharide revealed by the dominant lethal assay and micronucleus assays, and reproductive performance of male mice exposed to cyclophosphamide
Provedor de dados: 74
Autores: Oliveira,Rodrigo Juliano; Pesarini,João Renato; Salles,Maria José Sparça; Kanno,Tatiane Yumi Nakamura; Lourenço,Ana Carolina dos Santos; Leite,Véssia da Silva; Silva,Ariane Fernanda da; Matiazi,Hevenilton José; Ribeiro,Lúcia Regina; Mantovani,Mário Sérgio.
β-glucan is a well-known polysaccharide for its chemopreventive effect. This study aimed to evaluate the chemopreventive ability of β-glucan in somatic and germ cells through the dominant lethal and micronucleus assays, and its influence on the reproductive performance of male mice exposed to cyclophosphamide. The results indicate that β-glucan is capable of preventing changes in DNA in both germ cells and somatic ones. Changes in germ cells were evaluated by the dominant lethal assay and showed damage reduction percentages of 46.46% and 43.79% for the doses of 100 and 150 mg/kg. For the somatic changes, evaluated by micronucleus assay in peripheral blood cells in the first week of treatment, damage reduction percentages from 80.63-116.32% were found. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mutation; Post-implantation losses; Chemoprevention; Micronucleus; Nulliparous females.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100017

Genetic damage induced by trophic doses of lead in the neotropical fish Hoplias malabaricus (Characiformes, Erythrinidae) as revealed by the comet assay and chromosomal aberrations
Provedor de dados: 74
Autores: Cestari,Marta Margarete; Lemos,Priscilla Maria M.; Ribeiro,Ciro Alberto de Oliveira; Costa,João Ricardo M. Alves; Pelletier,Emilien; Ferraro,Marcos V.M.; Mantovani,Mário Sérgio; Fenocchio,Alberto Sergio.
The effects of clastogenic or mutagenic agents have rarely been studied in neotropical fish species exposed to contaminated water. In this study, the genetic damage caused by lead in the widely distributed South American fish, Hoplias malabaricus, was assessed using the comet (SCGE) assay and by testing for chromosomal aberrations. Eighteen specimens were acclimatized to laboratory conditions and then chronically exposed to contaminated food by feeding prey (Cyprinus sp.) injected intraperitoneally with doses of inorganic lead adjusted to give a contamination level of 21 mg of Pb2+.g-1 net weight of H. malabaricus. Three fish were sampled for chromosomal analysis after four doses (18 days) and another three after eight doses (41 days) of lead and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal aberrations; Comet assay; Genotoxicity; Hoplias malabaricus; Lead; Mutagenesis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200023

Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells
Provedor de dados: 74
Autores: Guterres,Zaira da Rosa; Mantovani,Mário Sérgio; Eira,Augusto Ferreira da; Ribeiro,Lúcia Regina; Jordão,Berenice Quinzani.
Agaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 -&gt; 6)beta-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN) and comet (single cell microgel electrophoresis) assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB) obtained from commercial sources or the following strains: a) strains AB 97/29 (young and sporulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus blazei; Micronuclei; Comet assay; V79 cells; Mushroom extracts.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300022