Consulta: creatorFacets:"Marques-Santos,Luis F."
Registros recuperados: 1
Data/hora: 05/06/2024 19:06:10

Ouabain exacerbates activation-induced cell death in human peripheral blood lymphocytes
Provedor de dados: 42
Autores: Esteves,Mabel B.; Marques-Santos,Luis F.; Affonso-Mitidieri,Ottília R.; Rumjanek,Vivian M.
Lymphocytes activated by mitogenic lectins display changes in transmembrane potential, an elevation in the cytoplasmic Ca2+ concentrations, proliferation and/or activation induced cell death. Low concentrations of ouabain (an inhibitor of Na+,K+-ATPase) suppress mitogen-induced proliferation and increases cell death. To understand the mechanisms involved, a number of parameters were analyzed using fluorescent probes and flow cytometry. The addition of 100nM ouabain to cultures of peripheral blood lymphocytes activated with 5µg/ml phytohemagglutinin (PHA) did not modify the increased expression of the Fas receptor or its ligand FasL induced by the mitogen. However, treatment with ouabain potentiated apoptosis induced by an anti-Fas agonist antibody. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphocytes; Ouabain; PHA; Apoptosis; Membrane depolarization.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000200008