Consulta: creatorFacets:"Martínez,José Gregorio"
Registros recuperados: 1
Data/hora: 06/06/2024 03:05:36

DNA fragmentation and membrane damage of bocachico Prochilodus magdalenae (Ostariophysi: Prochilodontidae) sperm following cryopreservation with dimethylsulfoxide and glucose
Provedor de dados: 83
Autores: Martínez,José Gregorio; García,Víctor Atencio; Carrasco,Sandra Pardo.
The endangered bocachico Prochilodus magdalenae is a native freshwater fish of Colombia, the most captured species locally and one of the most important species for ex-situ conservation (germplasm banks). The aim of this study was to examine the effect of three concentrations of Dimethylsulfoxide (DMSO) (5%, 10%, 15%) and three of glucose (305, 333, 361 mM) in the extender on spermatic DNA fragmentation (F-DNA) (by Halomax®, Chromatin dispersion) and membrane damage (D-Me) (by eosin-nigrosin staining). After assessment of sperm quality by computer analysis of motility, one part of semen from males was diluted separately with three parts of extender and filled into 0.5 ml straws. Freezing was carried out in liquid nitrogen vapor dry shipper for 30 minutes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Characiformes; Cryobiology; Cytotoxicity; Genotoxicity; Germplasm.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000300011