Consulta: creatorFacets:"Martins,A.S."
Registros recuperados: 12
Data/hora: 01/06/2024 08:36:50

Apoptose e expressão de Bcl-2 e das caspases 3 e 8 em placenta bovina, em diferentes estádios de gestação
Provedor de dados: 45
Autores: Meça,K.K.O.L.; Martins,A.S.; Del Puerto,H.L; Vasconcelos,A.C.
Apoptose e seus mecanismos reguladores são eventos fisiológicos cruciais para a manutenção da homeostase placentária, e o desequilíbrio desses processos pode comprometer a função placentária e, consequentemente, o sucesso da gravidez. Neste estudo, investigou-se a apoptose utilizando-se histomorfometria em lâminas coradas em HE e submetidas à reação de TUNEL. Além disso, avaliou-se a expressão de Bcl-2 e das caspases 8 e 3, pela reação de polimerase em cadeia em tempo real, em placentas saudáveis em diferentes estádios de gestação. Amostras de placentônios de vacas com quatro, seis e nove meses de gestação foram colhidas e processadas. O índice apoptótico aumentou progressivamente com o avanço da gestação. Tanto o Bcl-2 quanto as caspases 3 e 8 foram...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Apoptose; Placenta; Caspases; Bcl-2.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000200003

Presença de "Linha Morgan" como indicador de displasia coxofemoral em cães da raça Pastor-Alemão
Provedor de dados: 45
Autores: Tôrres,R.C.S.; Ferreira,P.M.; Araújo,R.B.; Martins,A.S.
The aim of this study was to determine the relationship between the presence of Morgan line and hip dysplasia. From June, 1986 to October, 1993, 190 X-ray plates of German Shepherd dogs, from both sexes were collected and analyzed. The results showed that out of the 190 dogs evaluated, 136 (71.6%) had hip dysplasia, 43 (22.6%) presented Morgan line and 41 (21.5%) showed both of them. Just two (1.1%) dogs presented Morgan line without hip dysplasia. No statistically significant difference was found between males (21.8%) and females (23.3%). From this study it can be concluded that the presence of the Morgan line can be considered indicative to hip dysplasia diagnosis, even though the absence of the line does not exclude the possibility of hip dysplasia.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Hip dysplasia; Radiology; Morgan line.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000200006

Morphometric analysis of the thymus of puppies infected with the Snyder Hill Strain of canine distemper virus
Provedor de dados: 45
Autores: Alves,C.M.; Vasconcelos,A.C.; Martins,A.S.; del Puerto,H.L.; Santos,F.G.A.; Nunes,J.E.S.; Campos,P.P.; Moro,L.
The thymic morphometry analysis was used for determining apoptosis and atrophy of the thymus of eight puppies inoculated with canine distemper virus (CDV). Three healthy dogs were used as negative controls. Sections, 5µm thick, were stained by HE and Shorr, and the latter were evaluated by morphometry. CDV nucleoprotein was detected by immunohistochemistry. Morphometric results confirmed lymphoid hypotrophy in CDV inoculated dog thymuses, more stroma, less parenchyma and higher apoptotic index/field than negative control (not inoculated) puppies. Apoptosis plays a role in the mechanism of thymus atrophy that develops in canine distemper.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Puppy; Apoptosis; Thymus; Canine distemper; Immunology; Virus; Immunosuppression.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400004

Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases
Provedor de dados: 45
Autores: Santos,F.G.A.; Vasconcelos,A.C.; Nunes,J.E.S.; Cassali,G.D.; Paixão,T.A.; Martins,A.S.; Silva,S.S.; Martins,R.F.; Moro,L.
Twelve male, mongrel, adult dogs were subcutaneously transplanted with cells originated from two canine transmissible venereal tumors (TVT). The aim was to demonstrate and to quantify the occurrence of apoptosis in the TVT regression. After six months of transplantation, a tumor sample was obtained from each dog, being six dogs with TVT in the growing phase and six in the regression phase as verified by daily measurements. Samples were processed for histological and ultrastructural purposes as well as for DNA extraction. Sections of 4µm were stained by HE, Shorr, methyl green pyronine, Van Gieson, TUNEL reaction and immunostained for P53. The Shorr stained sections went through morphometry that demonstrated an increase of the apoptotic cells per field in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Venereal tumor; Apoptosis; Cell death.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300013

Detection of different Brazilian strains of the bovine herpesvirus-1 (BHV-1) by polymerase chain reaction
Provedor de dados: 45
Autores: Cândido,A.L.; Martins,A.S.; Barros,P.B.; Resende,M.
A técnica da reação em cadeia pela DNA polimerase (PCR) foi usada para a amplificação rápida de um fragmento de 456bp da região única curta (Us) do genoma do BHV-1. Iniciadores de 18pb do gene da ORF1 foram usados para a amplificação das amostras-padrão e brasileiras. Uma amplificação clássica não foi bem sucedida. A amplificação foi obtida quando se escolheu uma região com baixa concentração de GC no DNA do BHV-1 e através da desnaturação térmica (95° C para 5min) seguida de ciclos térmicos (94° C por 1min e 30seg; 52° C por 1min; 72° C por 1min e 30seg; e ainda por 35 ciclos de 94° C por 1min; 52° C por 1min; 72° C por 1min e 30seg; usando um tempo de extensão final de 72° C por 5min). A clivagem com Pst I confirmou a especificidade do fragmento da ORF 1...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bovine herpesvirus 1; ORF-1; Polymerase chain reaction; Brazil.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000300007

Apoptose e expressão de VP2 e GAPDH na infecção precoce pelo vírus da doença infecciosa da bursa de Fabricius em pintos SPF
Provedor de dados: 45
Autores: Batista,J.J.; Martins,A.S.; Moro,L.; Resende,J.S.; Martins,N.R.S.; Vasconcelos,A.C.
Vinte e nove pintos SPF de um dia foram inoculados com o vírus da doença infecciosa da bursa de Fabricius (VDIB) para avaliar a ocorrência precoce de apoptose e a expressão da proteína viral 2 (VP2) e da enzima gliceraldeído fosfato dehidrogenase (GAPDH). Os animais foram distribuídos em cinco grupos: 1-controle; e 2 a 5- com 24, 48, 72 e 96 horas pós-inoculação, respectivamente. Fragmentos da bursa de Fabricius foram colhidos para processamento histológico e extração de RNA. Lâminas coradas em HE e TUNEL (marcação in situ da fragmentação do genoma com transferase terminal de deoxinucleotídeo) foram utilizadas na morfometria do índice apoptótico. Amostras de mRNA foram testadas para a expressão dos genes VP2 e GAPDH utilizando-se transcrição reversa e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptose; IBDV; Bursa de Fabricius; RT-PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200007

Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction
Provedor de dados: 45
Autores: Carvalho,R.; Passos,L.M.F.; Oliveira,A.M.; Henry,M.; Martins,A.S.
The genome of one equine embryo and three equine semen specimens collected from a Brazilian farm were tested by polymerase chain reaction (PCR) for the presence of EHV-1 and EHV-4-specific timidine kinase (TK) sequences. The PCR detected specific EHV-1 TK gene sequences in all samples tested. The peripheral blood leukocytes (PBL) of the embryo donor mare also was amplified by EHV-1 TK primers. Infectious virus was not recovered from any specimens. The animals did not show any clinical signal of EHV-1 or EHV-4 infections. EHV-4 was not detected in the studied specimens. The results indicate that PCR was more sensitive than virus isolation in cell culture for detecting EHV-1 in semen of carrier horses.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Equine; Semen; Embryo; EHV-1; EHV-4; PCR.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400002

Expressão gênica de caspases 3 e 8 em timo e baço de ratas recém-desmamadas e imunossuprimidas por glicocorticóide
Provedor de dados: 45
Autores: Batista,J.J.; Martins,A.S.; Moro,L.; Vasconcelos,A.C.
Determinou-se a expressão gênica das caspases 3 e 8 mediante transcrição reversa de mRNA total e reação em cadeia da polimerase (RT-PCR) para avaliar a apoptose em timo e baço de ratas imunossuprimidas por glicocorticóides. Utilizou-se dexametasona para indução da apoptose e atrofia linfóide. Quarenta e cinco fêmeas Wistar recém-desmamadas foram separadas em três grupos: as ratas de A (n=18) e B (n=18) foram tratadas com 250 e 500mg de glicocorticóide, via intramuscular, respectivamente, e as do C (n=9) não foram tratadas. Após 24, 48 e 72 horas, seis animais de cada grupo tratado e três do controle foram anestesiados, pesados e sacrificados. O baço e o timo foram coletados e pesados. Fragmentos dos órgãos foram fixados em formol tamponado a 10% e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rato; Apoptose; Dexametasona; Timo; Baço; Caspase 3; Caspase 8; RT-PCR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000400006

Etoposide-induced apoptosis in murine neuroblastoma (N2A) cells infected with Paramyxoviruses
Provedor de dados: 45
Autores: Moro,L.; Vasconcelos,A.C.; Martins,A.S.
The present study aimed to determine whether measles virus can induce apoptosis in murine neuroblastoma cells and the behavior of these cells under acute infection with measles virus or persistent infection with canine distemper virus upon treatment with etoposide. Measles virus induced necrosis in murine neuroblastoma cells. Canine distemper virus-persistent infection did not alter murine neuroblastoma cells behavior when treated with etoposide.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Measles virus; Canine distemper virus; Murine neuroblastoma cells.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000100007

Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction
Provedor de dados: 45
Autores: Cecílio,A.B.; Cândido,A.L.; Resende,M.; Bontempo,E.D.; Martins,A.S.
A reverse transcriptase polymerase chain reaction (RT-PCR) to detect mouse hepatitis virus (MHV) in hepatic tissue was developed. To circumvent possible failures in RT-PCR amplifications, a second round of PCR with internal primers was used to confirm the specificity and increase the sensitivity of the test. Using this method specific amplification of MHV sequences was observed in 18 out of 20 mouse colonies examined.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mouse hepatitis virus; RT-PCR.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400003

Use of an ELISA system for detection of equine herpesvirus 1 (EHV-1) antibodies in non-symptomatic pregnant mares and neonatal foals
Provedor de dados: 45
Autores: Carvalho,R.; Passos,L.M.F.; Gouvea,A.M.G.; Resende,M.; Martins,A.S.; Franco,G.C.
An ELISA system was applied to study the prevalence of EHV-1 in vaccinated and non-vaccinated Brazilian horses. Serum samples were taken from non-symptomatic pregnant mares, before and after the parturition, and from their neonatal foals. The antigen was prepared from a purified virus fraction (HVE-1 AB1 strain). The ELISA absorbance of 0.3516 was determined as the threshold of negative values. This cut-off level was defined from a selected interval, which determined a sensibility of 100% and a specificity of 94.7%, from a serum collection obtained from pregnant mares and fetuses. In this study, a strong correlation was found between the ELISA and serum neutralization for specific EHV-1 antibody detection.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horse; Herpesvirus; ELISA.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000300003

Experimental myocardial hypertrophy induced by a minimally invasive ascending aorta coarctation
Provedor de dados: 56
Autores: Martins,A.S.; Aguilera,N.W.; Matsubara,B.B.; Bregagnollo,E.A.
Ascending aorta coarctation was produced by a minimally invasive technique in rabbits. Animal mortality was 5%. Morphometric and hemodynamic parameters were evaluated. A parabiotically isolated heart model was used to assess the hemodynamic parameters. Left ventricular weight/body weight ratio and muscle area showed clear evidence of hypertrophy when compared to control. The hemodynamic changes in the isolated heart model suggested decreased diastolic and systolic function in the coarcted group. The present model produced hypertrophy with low mortality rates as a result of its less invasive nature.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ventricular hypertrophy; Ventricular function; Experimental model.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300017