Consulta: creatorFacets:"Melville,Priscilla Anne"
Registros recuperados: 5
Data/hora: 06/06/2024 18:15:03

EMJH medium with 5-fluorouracil and nalidixic acid associated with serial dilution technique used to recover Leptospira spp from experimentally contaminated bovine semen
Provedor de dados: 58
Autores: Miraglia,Fabiana; Moraes,Zenaide Maria de; Melville,Priscilla Anne; Dias,Ricardo Augusto; Vasconcellos,Silvio Arruda.
Bovine semen experimentally contaminated with Leptospira santarosai serovar Guaricura was submitted to the modified EMJH medium with 5-fluorouracil (300mg/L) and nalidixic acid (20mg/L), named as "selective medium" and using the serial dilution technique, in order to evaluate the percentage of recovery of the added microorganism. The selective EMJH medium was found with higher percentage of recovery of leptospiras and minor losses of samples due to contamination with opportunistic microorganisms than the non-selective EMJH medium: 151/376 (40.0%) of positive growth; and 38/376 (10.0%) contamination and 58/376 (15%) and 129/376 (34.0%), respectively. These results were statistically significant (p&lt;0. 0001; Fisher). Differences were found when the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospires; Culture media; Antimicrobial; Isolation; Semen.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100033

Mycobacterium bovis infection in goats from the Northeast region of Brazil
Provedor de dados: 58
Autores: Higino,Severino Silvano dos Santos; Pinheiro,Sônia Regina; Souza,Gisele Oliveira de; Dib,Cristina Corsi; Rosário,Tatiana Reis do; Melville,Priscilla Anne; Alves,Clebert José; Azevedo,Sérgio Santos de.
A total of 8,058 male and female mixed-breed goats and 1-4 years of age were slaughtered over a period of 7 months at the public slaughterhouse of Patos city, Paraíba state, in the Northeast region of Brazil; 822 animals were inspected for gross lesions of tuberculosis, and 12 (1.46%) had lesions suggestive of tuberculosis in the mammary gland, lungs, liver and mediastinal, mesenteric, submandibular, parotid and prescapular lymph nodes. Presence of granulomatous lesions was confirmed in the submandibular lymph node of one (8.3%) goat at the histopathological examination and at the mycobacterium culture the same sample was confirmed positive. Isolate was confirmed as belonging to the M. tuberculosis complex by PCR restriction enzyme analysis (PRA)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caprine tuberculosis; Mycobacterium culture; PCR restriction enzyme analysis; Spoligotyping.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400028

Microbiological and histopathological aspects of canine pyometra
Provedor de dados: 58
Autores: Coggan,Jennifer Anne; Melville,Priscilla Anne; Oliveira,Clair Motos de; Faustino,Marcelo; Moreno,Andréa Micke; Benites,Nilson Roberti.
As pyometra is recognized as one of the main causes of disease and death in the bitch the purposes of this study were to evaluate microbiological and histopathological aspects of canine pyometra and to research the virulence factors of the E. coli isolates identifying possible risks to human health. The microbiological isolation from the intrauterine contents of 100 dogs with pyometra was carried out and the virulence factors in the E. coli strains were identified using PCR method. This study also consisted of the counting of microorganisms colonies forming units in samples of intrauterine content, tests of antimicrobial susceptibility of the E. coli isolates and the histological examination of the uterus. E. coli was the most prevalent microorganism...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bitches; Escherichia coli; Histopathological; Microbiological; Pyometra.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300012

Comparison of four antibiotics for inactivating leptospires in bull semen diluted in egg yolk extender and experimentally inoculated with Leptospira santarosai serovar guaricura
Provedor de dados: 58
Autores: Miraglia,Fabiana; Morais,Zenaide Maria; Cortez,Adriana; Melville,Priscilla Anne; Marvullo,Maria Fernanda Vianna; Richtzenhain,Leonardo José; Visintin,José Antonio; Vasconcellos,Silvio Arruda.
Inactivation of leptospires in pools of semen from three Holstein Friesian bulls, collected in an artificial vagina, was investigated. Spermatic concentration was adjusted in egg yolk citrate extender, submitted to the following treatments: A (control; without antibiotics); B (penicillin, 1,000 UI/mL - streptomycin, 1,000 µg/mL); C (amoxicillin, 1,000 µg/mL); D (ceptiofur sodium, 1,000 µg/mL); E (amoxicillin 1,000 µg/mL - ceptiofur sodium 1,000 µg/mL). Leptospires (2.0 x 10(6) leptospires/mL) were added into the diluted semen. Recovery of leptospires was obtained in modified EMJH semi-solid medium with and without antibiotics. The antibiotics in the concentrations used did not affect means of percentage of progressive motility and individual progressive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospires; Semen; Antibiotics; Extender.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200011

Determinação da microbiota presente na cloaca e orofaringe de avestruzes (Struthio camelus) clinicamente sadios
Provedor de dados: 65
Autores: Melville,Priscilla Anne; Cogliati,Bruno; Mangiaterra,Maria Bárbara Baptista Cepellos Daruiz; Peres,Monica Ruz; Moura,Sílvio Carlos Alves; Matsuda,Letícia; Kim,Andrezza; Benites,Nilson Roberti.
O conhecimento da microbiota que compõe as diferentes áreas do organismo tem importância reconhecida para a compreensão de doenças infecciosas que podem acometer os avestruzes, embora se disponha de dados limitados acerca deste assunto na literatura. Foi objetivo deste estudo determinar as espécies de microrganismos (bactérias aeróbias e fungos) que compõem a microbiota normal de avestruzes. Para tanto, foram coletadas amostras de cloaca (N=50) e orofaringe (N=50) de avestruzes hígidos de um criadouro. Das amostras de cloaca, foram isolados Escherichia coli (76% das amostras positivas), Bacillus spp. (60%), Streptococcus spp. (18%), Staphylococcus coagulase-negativo (16%), Pseudomonas aeruginosa (8%), Rhodotorula spp. (8%), dentre outros microrganismos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Avestruz; Cloaca; Microbiota; Orofaringe; Patologia aviária.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000600031