Consulta: creatorFacets:"Mesquita,Rosilene Oliveira"
Registros recuperados: 5
Data/hora: 06/06/2024 07:34:00

Expression analysis in response to drought stress in soybean: shedding light on the regulation of metabolic pathway genes
Provedor de dados: 74
Autores: Guimarães-Dias,Fábia; Neves-Borges,Anna Cristina; Viana,Antonio Americo Barbosa; Mesquita,Rosilene Oliveira; Romano,Eduardo; Grossi-de-Sá,Maria de Fátima; Nepomuceno,Alexandre Lima; Loureiro,Marcelo Ehlers; Alves-Ferreira,Márcio.
Metabolomics analysis of wild type Arabidopsis thaliana plants, under control and drought stress conditions revealed several metabolic pathways that are induced under water deficit. The metabolic response to drought stress is also associated with ABA dependent and independent pathways, allowing a better understanding of the molecular mechanisms in this model plant. Through combining an in silico approach and gene expression analysis by quantitative real-time PCR, the present work aims at identifying genes of soybean metabolic pathways potentially associated with water deficit. Digital expression patterns of Arabidopsis genes, which were selected based on the basis of literature reports, were evaluated under drought stress condition by Genevestigator. Genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Drought resistance; QPCR; Metabolic pathway; Bioinformatics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200004

Method optimization for proteomic analysis of soybean leaf: improvements in identification of new and low-abundance proteins
Provedor de dados: 74
Autores: Mesquita,Rosilene Oliveira; Soares,Eduardo de Almeida; Barros,Everaldo Gonçalves de; Loureiro,Marcelo Ehlers.
The most critical step in any proteomic study is protein extraction and sample preparation. Better solubilization increases the separation and resolution of gels, allowing identification of a higher number of proteins and more accurate quantitation of differences in gene expression. Despite the existence of published results for the optimization of proteomic analyses of soybean seeds, no comparable data are available for proteomic studies of soybean leaf tissue. In this work we have tested the effects of modification of a TCA-acetone method on the resolution of 2-DE gels of leaves and roots of soybean. Better focusing was obtained when both mercaptoethanol and dithiothreitol were used in the extraction buffer simultaneously. Increasing the number of washes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proteomics; Glycine max; 2-DE; Protein extraction; Leaf proteome.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200017

Expression pattern of drought stress marker genes in soybean roots under two water deficit systems
Provedor de dados: 74
Autores: Neves-Borges,Anna Cristina; Guimarães-Dias,Fábia; Cruz,Fernanda; Mesquita,Rosilene Oliveira; Nepomuceno,Alexandre Lima; Romano,Eduardo; Loureiro,Marcelo Ehlers; Grossi-de-Sá,Maria de Fátima; Alves-Ferreira,Márcio.
The study of tolerance mechanisms for drought stress in soybean is fundamental to the understanding and development of tolerant varieties. Using in silico analysis, four marker genes involved in the classical ABA-dependent and ABA-independent pathways of drought response were identified in the Glycine max genome in the present work. The expression profiles of the marker genes ERD1-like, GmaxRD20A-like, GmaxRD22-like and GmaxRD29B-like were investigated by qPCR in root samples of drought sensitive and tolerant soybean cultivars (BR 16 and Embrapa 48, respectively), submitted to water deficit conditions in hydroponic and pot-based systems. Among the four putative soybean homologs to Arabidopsis genes investigated herein, only GmaxRD29B-like was not regulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water deficit; Drought adaptation; Marker genes; Glycine max.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200003

Gas exchanges and growth of maize as affected by aeration porosity and soil compaction
Provedor de dados: 124
Autores: Silva,Francisca Gleiciane da; Assis Junior,Raimundo Nonato de; Mesquita,Rosilene Oliveira; Marques,Edilaine da Silva; Mota,Jaedson Cláudio Anunciato.
ABSTRACT Based on the hypothesis that less than 10% aeration porosity is sufficient to ensure plant development, the objective of this study was to evaluate the effect of levels of aeration porosity and soil density on the gas exchange and growth of maize plants. The experiment was conducted in a greenhouse, in randomized blocks in a 2 × 5 factorial arrangement: two densities (1.6 and 1.7 Mg m-3) and five aeration porosities (0.07, 0.08, 0.09, 0.10 and 0.12 m3 m-3), with five replicates. The interaction between the factors did not have an effect on the analyzed variables. Aeration porosity had a significant effect on photosynthesis, stomatal conductance, relative chlorophyll index (SPAD), plant height and biomass production, whose data were fitted by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soil aeration; Physical quality; Ecophysiology; Zea mays L.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000300403

Growth and gas exchange in white pitaya under different concentrations of potassium and calcium
Provedor de dados: 124
Autores: Cajazeira,João Paulo; Corrêa,Márcio Cleber de Medeiros; Almeida,Edmilson Igor Bernardo; Queiroz,Ronialison Fernandes; Mesquita,Rosilene Oliveira.
ABSTRACT Agriculture in Brazil has improved at a fast pace in recent years, given the growing demand for quality and the need for new products. In this respect, white pitaya [Hylocereus undatus (Haw.) Britton &amp; Rose] has become a feasible alternative for Northeast farmers. The limiting factors include a small amount of data on plant mineral nutrition and crop growth (phenology). Therefore, this study goal was to evaluate the effect of different concentrations of potassium (K) and calcium (Ca) on crop development and gas exchange in white pitaya grown in the coastal region of the state of Ceará, in Brazil. Sixteen treatments with three repetitions were organized in a completely randomized block design and a 4 × 4 factorial arrangement. Treatments...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hylocereus undatus; Cactaceae; Nutrient interaction; Mineral nutrition.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902018000100112