Consulta: creatorFacets:"Millán,J.L."
Registros recuperados: 2
Data/hora: 15/06/2024 14:19:14

Contribution of matrix vesicles and alkaline phosphatase to ectopic bone formation
Provedor de dados: 56
Autores: Ciancaglini,P.; Simão,A.M.S.; Camolezi,F.L.; Millán,J.L.; Pizauro,J.M.
Endochondral calcification involves the participation of matrix vesicles (MVs), but it remains unclear whether calcification ectopically induced by implants of demineralized bone matrix also proceeds via MVs. Ectopic bone formation was induced by implanting rat demineralized diaphyseal bone matrix into the dorsal subcutaneous tissue of Wistar rats and was examined histologically and biochemically. Budding of MVs from chondrocytes was observed to serve as nucleation sites for mineralization during induced ectopic osteogenesis, presenting a diameter with Gaussian distribution with a median of 306 ± 103 nm. While the role of tissue-nonspecific alkaline phosphatase (TNAP) during mineralization involves hydrolysis of inorganic pyrophosphate (PPi), it is unclear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Matrix vesicles; Endochondral ossification; Osseous plate; Alkaline phosphatase; Ectopic mineralization; Calcification.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500006

Proteoliposomes as matrix vesicles' biomimetics to study the initiation of skeletal mineralization
Provedor de dados: 56
Autores: Simão,A.M.S.; Yadav,M.C.; Ciancaglini,P.; Millán,J.L.
During the process of endochondral bone formation, chondrocytes and osteoblasts mineralize their extracellular matrix by promoting the formation of hydroxyapatite (HA) seed crystals in the sheltered interior of membrane-limited matrix vesicles (MVs). Ion transporters control the availability of phosphate and calcium needed for HA deposition. The lipidic microenvironment in which MV-associated enzymes and transporters function plays a crucial physiological role and must be taken into account when attempting to elucidate their interplay during the initiation of biomineralization. In this short mini-review, we discuss the potential use of proteoliposome systems as chondrocyte- and osteoblast-derived MVs biomimetics, as a means of reconstituting a phospholipid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alkaline phosphatase; Biomineralization; Calcification; Lipids; Pyrophosphate; ATP.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300003