Consulta: creatorFacets:"Miyamoto, Akio"
Registros recuperados: 31
Data/hora: 05/06/2024 02:30:57

ウシの自発的な黄体退行中の黄体内血流変動の経時的観察
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2164

Relationship between metabolic hormones and ovulation of dominant follicle during the first follicular wave post-partum in high-producing dairy cows
Provedor de dados: 19
Autores: Kawashima, Chiho; Fukihara, Saori; Maeda, Mayumi; Kaneko, Etsushi; Montoya, Carlos Amaya; Matsui, Motozumi; Shimizu, Takashi; Matsunaga, Nobuyoshi; Kida, Katsuya; Miyake, Yoichi; Schams, Dieter; Miyamoto, Akio; 川島, 千帆; 松井, 基純; 清水, 隆; 木田, 克弥; 三宅, 陽一; 宮本, 明夫.
Recent studies suggest that IGF-I is a crucial regulatory factor in follicular growth during early post-partum period. The aim of the present study was to determine in detail the changing profiles of metabolic and reproductive hormones in relation to ovulation of the dominant follicle (DF) of the first follicular wave post-partum in high-producing dairy cows. Plasma concentrations of related hormones in 22 multiparous Holstein cows were measured from 4 weeks pre-partum to 3 weeks post-partum, and the development of DF was observed with colour Doppler ultrasound. Thirteen cows showed ovulation by 15.2 days post-partum. Anovulatory cows showed higher GH and lower IGF-I levels than those in ovulatory cows during the peri-partum period. Each DF developed...
Palavras-chave: GROWTH-FACTOR-I; COMPETITIVE ENZYME-IMMUNOASSAY; ENERGY-BALANCE; OVARIAN ACTIVITY; STIMULATING-HORMONE; ESTRADIOL PRODUCTION; BODY CONDITION; MESSENGER-RNA; HOLSTEIN COWS; IGF-I.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1658

Association of rumen fill score and energy status during the close-up dry period with conception at first artificial insemination in dairy cows
Provedor de dados: 19
Autores: Kawashima, Chiho; Karaki, Chihiro; Munakata, Megumi; Matsui, Motozumi; Shimizu, Takashi; Miyamoto, Akio; Kida, Katsuya.
Recent studies have shown significant associations between prepartum energy status and postpartum fertility in dairy cows; therefore, the assessment of energy status by blood metabolites and metabolic hormones and suitable improvement of management during the prepartum period may enhance reproductive performance. Rumen fill score (RFS) is associated with feed intake; however, it is unknown whether RFS is also related to blood parameters. Therefore, this study investigated the relationship between RFS and energy status during the prepartum period, and their associations with conception at first artificial insemination (AI) after parturition. In 42 multiparous Holstein cows, RFS assessment and blood sampling were carried out twice a week during 3 weeks of...
Palavras-chave: Dairy cow; Dry period; First artificial insemination; Rumen fill score.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4529

ウシ卵胞腔内への生理活性物質投与(VEGF抗体)による卵巣機能制御法およびウシ黄体における経時的バイオプシーによるサンプル採取法の開発についての検討
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2178

Effect of Bovine Somatotropin Administration on Day 11 after Artificial Insemination in Estrus-Induced Ewes during the Non-Breeding Season
Provedor de dados: 19
Autores: Fukui, Yutaka; Yamamura, Kunio; Matsunaga, Nobuyoshi; Miyamoto, Akio; 福井, 豊; 宮本, 明夫.
The present study aimed to examine the possibility that bovine somatotropin (BST) treatment after artificial insemination (AI) may improve the fertility by the stimulation of corpus luteum (CL) function in seasonally anestrous ewes. The experiment was conducted at the Tawa Field Station, Shibecha-cho in Hokkaido, Japan, during the non-breeding season (May, 1995). Eighty-nine mature (2 to 3 years old) Suffolk and Suffolk-crossed ewes were treated with controlled internal drug release devices containing 0.3 g progesterone (CIDR-G) for 12 days. Ewes were injected intramuscularly with 500 IU PMSG the day before CIDR removal. Eighty-seven ewes were assigned to an insemination of frozen semen on a fixed-time basis (42 to 50 h) after removal of CIDR. On the 11th...
Palavras-chave: Bovine somatotropin; Artificial insemination; Progesterone; Conception rate; Ewes.
Ano: 2000
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2971

ウシ黄体内への血管作動性物質(ET-1アンタゴニスト)の投与による卵巣機能制御法の開発
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2159

Metabolic Profiles in Ovulatory and Anovulatory Primiparous Dairy Cows During the First Follicular Wave Postpartum
Provedor de dados: 19
Autores: Kawashima, Chiho; Sakaguchi, Minoru; Suzuki, Takahiro; Sasamoto, Yoshihiko; Takahashi, Yoshiyuki; Matsui, Motozumi; Miyamoto, Akio.
Metabolic hormones affect ovarian function in the cow. However, the relationship between metabolic factors and ovarian function is not clear in the postpartum primiparous cow because they are still growing. The aim of the present study was to investigate in detail the time-dependent profile of the metabolic hormones, metabolites, and milk yields of ovulatory and anovulatory primiparous cows during the first follicular wave postpartum. We used 16 primiparous Holstein cows and obtained blood samples for the profiles of metabolites (glucose; non-esterified fatty acid, NEFA; ketone body; total cholesterol; and aspartate aminotransferase), metabolic hormones (growth hormone, GH; insulin-like growth factor-I, IGF-1; and insulin), and progesterone every other day...
Palavras-chave: First follicular wave; Metabolic profile; Postpartum; Primiparous cow.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/921

主席卵胞の内因性LHサージへの暴露が血管新生を伴うウシ黄体の形成を引き起こす
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2173

ウシ優性卵胞と閉鎖卵胞の卵胞液中におけるN-アセチルグルコサミニルトランスフェラーゼ I 活性の解析
Provedor de dados: 19
Autores: Nozaki, Hirofumi; Miyamoto, Akio; Hayashi, Ken-Go; Matsui, Motozumi; Yoshida, Takashi; Nakamura, Tadashi; Arai, Ikichi; Urashima, Tadasu; 宮本, 明夫; 松井, 基純; 中村, 正; 浦島, 匡.
The activity of α3-D-mannoside-β-1,2-N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was detected in bovine follicular fluid (bFF). The GnT I activity in bFF had a pH optimum of 5.8 and an absolute requirement for either Co2+, Mn2+, or Mg2+, the activity being stimulated by these cations in the above order. The apparent Km value for α1-3α1-6 mannopentaose of GnT I in bFF was 2.17 mM. The substrate specificity for the GnT I activity decreased in the following order: α1-3α1-6 mannopentaose&gt; α1-3α1-6 mannotriose&gt;α1-3 mannobiose. The GnT I activity in bFF from large atretic follicles was significantly higher than in that from large...
Palavras-chave: N-acetylglucosaminyltransferase I; Dominant follicle; Atretic follicle; Follicular fluid; Bovine.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4185

LHサージ直前の排卵前卵胞の吸引による2個の優勢卵胞の発育時の､血中LHおよびFSH濃度の測定
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2168

An autoregressive logistic model to predict the reciprocal effects of oviductal fluid components on in vitro spermophagy by neutrophils in cattle
Provedor de dados: 19
Autores: Kowsar, Rasoul; Keshtegar, Behrooz; Marey, Mohamed. A.; Miyamoto, Akio.
After intercourse/insemination, large numbers of sperm are deposited in the female reproductive tract (FRT), triggering a massive recruitment of neutrophils (PMNs) into the FRT, possibly to eliminate excessive sperm via phagocytosis. Some bovine oviductal fluid components (BOFCs) have been shown to regulate in vitro sperm phagocytosis (spermophagy) by PMNs. The modeling approach-based logistic regression (LR) and autoregressive logistic regression (ALR) can be used to predict the behavior of complex biological systems. We, first, compared the LR and ALR models using in vitro data to find which of them provides a better prediction of in vitro spermophagy in bovine. Then, the best model was used to identify and classify the reciprocal effects of BOFCs in...
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4438

乳牛の受胎率向上を目的とした分娩前後の健康管理と繁殖モニタリングシステムの構築
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2172

ウシ卵巣内の血管新生と血管機能 : 遺伝子導入を利用した卵巣機能調節の多元的解析
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2165

Immunoreactivity for Gonadotropin-Releasing Hormone in Microdialyzed Perfusates of Bovine Mature Follicles In Vitro
Provedor de dados: 19
Autores: Ozawa, Takahiro; Acosta, Tomas J.; Nakamura, Tadashi; Miyamoto, Akio; 中村, 正; 宮本, 明夫.
The presence of gonadotropin-releasing hormone (GnRH)-like protein has been previously demonstrated in the bovine follicle. This GnRH-like protein was purified and concluded to be histone H2A. However, neither GnRH peptide nor specific GnRH-immunoreactivity (GnRH-IR) has been demonstrated in the bovine follicle so far. Thus, this study focused on the detection of specific GnRH-IR using the second-antibody enzyme immunoassay in isolated bovine mature follicles, and on an examination of the direct effect of luteinizing hormone (LH), endothelin-1 (ET-1), and cytokines on the GnRH-IR using an in vitro microdialysis system (MDS). We further examined a cross-reactivity of the GnRH antibody with bovine histone H2A. GnRH-IR was detected in microdialyzed perfusate...
Palavras-chave: GnRH; Immunoreactivity; Microdialysis; Follicle; Cow.
Ano: 2000
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2970

内因性LHサージの排卵(卵胞液除去)後の黄体形成過程へのインパクト
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2158

ウシ卵胞腔内への生理的活性物質投与(VEGF抗体)による卵巣機能制御法およびウシ黄体における経時的バイオプシーによるサンプル採取法の開発についての検討
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2163

乳牛における分娩後の最初の卵胞波の主席卵胞の排卵に関わる代謝要因と卵胞機能の解析
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
平成18年度 Ⅰ.基盤研究部門
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2570

Sperm Binding to Oviduct Epithelial Cells Enhances TGFB1 and IL10 Expressions in Epithelial Cells as Well as Neutrophils In Vitro: Prostaglandin E-2 As a Main Regulator of Anti-Inflammatory Response in the Bovine Oviduct
Provedor de dados: 19
Autores: Yousef, Mohamed Samy; Marey, Mohamed Ali; Hambruch, Nina; Hayakawa, Hiroyuki; Shimizu, Takashi; Hussien, Hassan Ali; Abdel-Razek, Abdel-Razek Khalifa; Pfarrer, Christiane; Miyamoto, Akio.
Sperm are allogenic to the female genital tract; however, oviducts provide optimal conditions for survival and capacitation of these non-self cells until fertilization. Recently, we showed that oviduct-conditioned media and prostaglandin E-2 (PGE(2)) suppress sperm phagocytosis by polymorphonuclear neutrophils (PMNs) under physiological conditions. We hypothesized that sperm binding to bovine oviduct epithelial cells (BOECs) could change the local innate immunity via PGE2. As the first step to obtain basic information, sub-confluent BOEC monolayers were co-cultured with swim-up sperm for 2 h. BOECs with viable bound sperm were cultured for an additional 3, 6, 12, or 24 h. Then, we confirmed the impact of the sperm-BOEC binding on both BOECs and PMN gene...
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4418

Effect of intraluteal injection of endothelin type A receptor antagonist on PGF2alpha-induced luteolysis in the cow.
Provedor de dados: 19
Autores: Watanabe, Sho; Shirasuna, Koumei; Matsui, Motozumi; Yamamoto, Dai; Berisha, Bajram; Schams, Dieter; Miyamoto, Akio; 白砂, 孔明; 松井, 基純; 宮本, 明夫.
Endothelin-1 (ET-1) is a luteolytic mediator in the bovine corpus luteum (CL), and its action appears to be via endothelin type A receptor (ETR-A). Thus, the aim of the present study was to determine the effect of ETR-A antagonist on PGF(2)alpha-induced luteolysis in the cow. Cows on days 1012 of the estrous cycle were subjected to five intraluteal injections of the ETR-A antagonist LU 135252 in saline or only saline at -0.5, 2, 4, 6, and 8 h after PGF(2)alpha administration (=0 h). Serial luteal biopsies were conducted to determine the expression of mRNA in the luteal tissue. There were no significant differences in the decrease in plasma progesterone (P) concentrations and the mRNA expressions of steroidogenic acute regulatory protein and 3...
Palavras-chave: Apoptosis; Blood flow; Corpus luteum; Cow; Endothelin-1.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/922

ホルスタイン種非搾乳牛におけるステロイドホルモン処置が代謝ホルモン分泌に及ぼす影響
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
平成18年度 Ⅰ.基盤研究部門
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2569

LHサージ直前の排卵前卵胞の吸引による「ダブル排卵」の誘起と､2個黄体の形成によるET受胎率の向上
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2162

カラードップラー超音波画像診断装置を用いたウシ卵胞の発育､閉鎖過程の血流像の動態
Provedor de dados: 19
Autores: 宮本, 明夫; 大谷, 昌之; Miyamoto, Akio; Otani, Masayuki.
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2157

ウシ黄体内へのendothelin type A receptor　(ETR-A) antagoinst投与がPGF₂α によって誘起された黄体退行に与える影響の観察
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2167

酵素免疫測定法によるウシの血漿中のインシュリン濃度の測定
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2161

乳牛の受胎率向上を目的とした分娩直後の健康管理と繁殖モニタリングシステムの構築
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2169

ウシ黄体内への生理活性物質投与(bFGF抗体)による卵巣機能制御法についての検討
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2171

LHサージ直前の排卵前卵胞の吸引により発育が引き起こされる2個の優勢卵胞の､卵胞液中ステロイド濃度等各種因子の解析
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2170

乾乳期における妊娠牛のインスリン感受性を中心にした血糖調節に関わる内分泌状況の評価
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2166

A cooperative action of endothelin-1 with prostaglandin F-2 alpha on luteal function in the cow
Provedor de dados: 19
Autores: Shirasuna, Koumei; Watanabe, S.; Oki, N.; Wijayagunawardane, M. P. B.; Matsui, Motozumi; Ohtani, M.; Miyamoto, Akio; 白砂, 孔明; 松井, 基純; 宮本, 明夫.
Prostaglandin F-2 alpha (PGF(2 alpha)) is the primary luteolysin in the cow, and luteal endothelin-1 (ET-1) interacts with PGF(2 alpha) during the process of luteolysis. In contrast, a developing corpus luteum (CL) is refractory to exogenous administration of PGF(2 alpha). Thus, the present study was aimed to investigate the functional relationship between ET-1 and PGF(2 alpha) in the mid-CL (PGF(2 alpha)-sensitive) and early-CL (PGF(2 alpha)-refractory). In the mid-CL model, cows (n = 6/treatment) were assigned to receive one of five types of treatments on day 10 of the estrous cycle: (1) an injection of saline; control, (2) a 500 jig of PGF2. analogue (sufficient dose to induce luteolytis); full-PG, (3) an intraluteal injection of 0.25 mg ET-1; ET-1, (4)...
Palavras-chave: Endothelin-1; Prostaglandin F-2 alpha; Luteolysis; Corpus luteum; Cow.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/825

Bovine embryo induces an anti-inflammatory response in uterine epithelial cells and immune cells in vitro: Possible involvement of interferon tau as an intermediator
Provedor de dados: 19
Autores: Talukder, Anup K.; Yousef, Mohamed S.; Rashid, Mohammad B.; Awai, Kensuke; Acosta, Tomas J.; Shimizu, Takashi; Okuda, Kiyoshi; Shimada, Masayuki; Imakawa, Kazuhiko; Miyamoto, Akio.
Recent observations suggest that the bovine uterus starts to react to the early embryo immediately after its arrival from the oviduct. The present study aimed to investigate the effect of the early developing embryo on the immune-related gene profile in bovine uterine epithelial cells (BUECs) in vitro, and to further examine the impact of conditioned media (CM), either from embryo-BUEC co-culture or embryo culture alone, on gene expression in peripheral blood mononuclear cells (PBMCs). First, BUECs were co-cultured with morulae (n = 10) for D5-D9 (D0 = IVF), and gene expression in BUECs was analyzed. Subsequently, PBMCs were cultured in CM from embryo-BUEC co-culture or D5-D9 embryo culture, and gene expression was evaluated. In BUECs, the embryo induced...
Palavras-chave: Bovine; Embryo; Immunity; Interferon tau (IFNT); Uterus.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4532

ウシ中期黄体におけるbFGF(線維芽細胞増殖因子)の作用の解明
Provedor de dados: 19
Autores: 宮本, 明夫; Miyamoto, Akio.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2177