Consulta: creatorFacets:"Montanie, Hélène"
Registros recuperados: 3
Data/hora: 07/06/2024 01:48:14

Detection of ostreid herpesvirus 1 (OsHV-1) DNA in seawater by PCR: influence of water parameters in bioassays
Provedor de dados: 5
Autores: Vigneron, Vassilia; Solliec, Gaelle; Montanie, Hélène; Renault, Tristan.
Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses; in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus I (OsHV-1) detection by polymerase chain reaction (PCR). Viral DNA extracted from purified particles or virions present in infected oyster larvae were detected by PCR after storage in different media at different temperatures. The lowest detection threshold was found using distilled water or Tris EDTA buffer. In seawater, the threshold was higher. The use of sterile media permitted...
Tipo: Text
Palavras-chave: Temperature; PCR; Detection; Seawater; Viral DNA; Oyster herpesvirus 1.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-2909.pdf

Impact of the oyster Crassostrea gigas on a microbial community in Atlantic coastal ponds near La Rochelle
Provedor de dados: 5
Autores: Dupuy, Christine; Pastoureaud, Annie; Ryckaert, Mireille; Sauriau, Pierre-guy; Montanie, Hélène.
To assess the in situ impact of oysters Crassostrea gigas on planktonic protist and bacteria communities and the potential contribution of protozoa to their food resource intake, the abundance and the diversity of protists and bacteria were followed in 2 Atlantic coastal ponds, with and without oysters. The protist biomass in such ponds was high, with a maximum in spring of 982 mug C l(-1) and a minimum in winter of 179 pg C l(-1). Whatever the season, the presence of oysters (20 m(-2) corresponding to an average of 23 mg dry weight m(-2)) induced a significant decrease in &gt;5 pm protist abundance. On the contrary, planktonic organisms &lt;5 pm, such as Chlorophyta flagellates and bacteria, developed similarly in both ponds. It can be assumed that such...
Tipo: Text
Palavras-chave: Trophic link; Bacteria; Protists; Microbial food web; Coastal pond; Food source; Oysters; Bivalve.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-822.pdf

Recherche d'AND d'herpèsvirus infectant les bivalves dans des échantillons d'eau de claires ostréicoles
Provedor de dados: 5
Autores: Solliec, Gaëlle; Vigneron, Valérie; Montanie, Hélène; Renault, Tristan.
Viral particles, associated with high mortality rates, have been reported among larvae and juveniles in different marine bivalve specie and characterized as member of the Hespesviridae family. Most studies focused on the detection of such viral infections among economically important species. However, the persistence of bivalve herpes viruses in marine environment is poorly documented. This topic appears as an important research domain in order to clarify the natural infection cycle of the herpesvirus of marine bivalves.
Tipo: Text
Palavras-chave: Herpesviridae; Oyster; PCR; DNA; Herpesvirus; Huître; PCR; ADN.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-2856.pdf