Consulta: creatorFacets:"Moraes,D.W."
Registros recuperados: 1
Data/hora: 05/06/2024 06:33:47

Evaluation of an anti-carcinoembryonic monoclonal antibody suitable for immunoscintigraphy
Provedor de dados: 56
Autores: Moraes,D.W.; Lima,E.N.P.; Prado,I.B.; Carneiro,C.R.W.
An anti-carcinoembryonic antigen (CEA) monoclonal antibody (mAb 6D1.1) was evaluated in vitro and in vivo to determine its suitability as a tracer for immunoscintigraphy of colorectal carcinomas. Determination of mAb affinity for CEA showed a constant of association of 0.63 ± 0.11 x 109 M-1. Binding of technetium-99m (99mTc)-6D1.1, labeled by a direct method, to human cultured lineages was highly specific. Binding to only CEA-positive LS-174T cells resulted in a saturable curve inhibited by pre-incubation with unlabeled mAb. No binding at all was observed for the human lineages MeWo (melanoma) or ZR75-30 (breast carcinoma), neither of them expressing CEA cells. Intravenous injection of 99mTc-6D1.1 into nude mice xenografted with human LS-174T tumors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monoclonal antibody; Carcinoembryonic antigen; Colorectal carcinoma; Immunoscintigraphy; Technetium-99m.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800006