Consulta: creatorFacets:"Moreira-Filho,Orlando"
Registros recuperados: 19
Data/hora: 05/06/2024 00:59:14

Dispersion of transposable elements and multigene families: Microstructural variation in Characidium (Characiformes: Crenuchidae) genomes
Provedor de dados: 74
Autores: Pucci,Marcela Baer; Nogaroto,Viviane; Moreira-Filho,Orlando; Vicari,Marcelo Ricardo.
Abstract Eukaryotic genomes consist of several repetitive DNAs, including dispersed DNA sequences that move between chromosome sites, tandem repeats of DNA sequences, and multigene families. In this study, repeated sequences isolated from the genome of Characidium gomesi were analyzed and mapped to chromosomes in Characidium zebra and specimens from two populations of C. gomesi. The sequences were transposable elements (TEs) named retroelement of Xiphophorus (Rex); multigene families of U2 small nuclear RNA (U2 snRNA); and histones H1, H3, and H4. Sequence analyses revealed that U2 snRNA contains a major portion corresponding to the Tx1-type non-LTR retrotransposon Keno, the preferential insertion sites of which are U2 snRNA sequences. All histone...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mobile DNA; Histones; Karyotype evolution; SnRNA; WZ/ZZ.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400585

Chromosomal distribution of the As51 satellite DNA in two species complexes of the genus Astyanax (Pisces, Characidae)
Provedor de dados: 74
Autores: Abel,Luciano Douglas dos Santos; Mantovani,Monique; Moreira-Filho,Orlando.
The chromosomal localization of the As51 satellite DNA was identified by fluorescent in situ hybridization (FISH) in specimens of the characid fish Astyanax scabripinnis and Astyanax fasciatus, which are considered species complexes because of their extensive karyotypical and morphological variability. A conserved chromosomal distribution of the As51 satellite, coincident with distal C-banded segments was demonstrated. The alternative interstitial localization of this satellite DNA and possible alterations of its structure suggest that this sequence underwent quantitative, positional and structural variations, as the A. scabripinnis and A. fasciatus complexes diverged.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: As51; Astyanax scabripinnis; Astyanax fasciatus; Fluorescence in situ hybridization (FISH); C-banding; Satellite DNA.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300008

Karyotypic differentiation through chromosome fusion and number reduction in Imparfinis hollandi (Ostariophysi, Heptapteridae)
Provedor de dados: 74
Autores: Margarido,Vladimir Pavan; Moreira-Filho,Orlando.
The Neotropical Heptapteridae fish Imparfinis hollandi, endemic to the Iguaçu River Basin (Brazil), was cytogenetically analyzed and the diploid chromosome number of 2n = 42 chromosomes was determined (22m + 10sm + 10st), the lowest diploid number in this genus and family. Like other Heptapteridae species, only one NOR-bearing chromosome pair was detected by silver nitrate staining. Dark heterochromatic blocks were visualized in only three chromosome pairs, and chromomycin A3+ bands were coincident with Ag-NORs. Although no intercalary (TTAGGG)n sequence was observed through FISH with a telomere probe, an asymmetric karyotype showing four large chromosome pairs with diploid chromosome number reduction suggests that tandem chromosome fusions probably...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Siluriformes; Chromosome banding; Chromosomal rearrangements; (TTAGGG)n.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200012

Evidence of the differentiated structural arrangement of constitutive heterochromatin between two populations of Astyanax scabripinnis (Pisces, Characidae)
Provedor de dados: 74
Autores: Mantovani,Monique; Abel,Luciano Douglas dos Santos; Mestriner,Carlos Alberto; Moreira-Filho,Orlando.
The composition of heterochromatin classes along the chromosomes of specimens from two populations of the fish Astyanax scabripinnis was examined using fluorescence banding with GC- and AT-DNA specific fluorochromes and fluorescence in situ hybridization (FISH) with an AT-rich satellite DNA (As51) probe. For the pericentromeric heterochromatin blocks neither GC/AT-DNA specific fluorochromes nor the FISH technique produce any response with chromosomes from either of the populations. On the other hand, the telomeric distal heterochromatin blocks of both populations fluoresced when the FISH technique was applied but showed distinct responses after GC-specific fluorochrome treatments, leading us to propose different structural arrangements of the FISH-positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Satellite DNA; Chromosome structure; Base-specific fluorochromes; Heterochromatin heterogeneity; Fluorescence in situ hybridization.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400012

Cytogenetic analysis and chromosomal characteristics of the polymorphic 18S rDNA in the fish Prochilodus lineatus (Characiformes, Prochilodontidae)
Provedor de dados: 74
Autores: Vicari,Marcelo Ricardo; Almeida,Mara Cristina de; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando; Artoni,Roberto Ferreira.
We used differential staining techniques (BSG, GTG, AgNO3, DAPI and CMA3 banding) and fluorescent in situ hybridization (FISH) with 5S and 18S probes to investigated the karyotypic and cytogenetic chracteristics of Prochilodus lineatus specimens from a population in Vila Velha state park (Parque Estadual de Vila Velha, Ponta Grossa, Paraná state, southern Brazil). The specimens studied showed the same karyotype as that found in other P. lineatus populations, i.e. 2n = 54 biarmed chromosomes (40m + 14 sm) and c-positive heterochromatin preferentially located pericentromerically in all chromosomes. The presence of partial or totally heterochromatic supernumerary chromosomes with numeric intra-individual variation was confirmed in the analyzed population. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish cytogenetics; Prochilodus; Karyotype; Minor and major rDNA; NOR polymorphism.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400008

Cytogenetic analyses of two endemic fish species from the São Francisco River basin: Conorhynchus conirostris and Lophiosilurus alexandri (Siluriformes)
Provedor de dados: 74
Autores: Marques,Marilza Barbosa de Almeida; Moreira-Filho,Orlando; Garcia,Caroline; Margarido,Vladimir Pavan.
Two Siluriformes species endemic to the São Francisco River basin were characterized by conventional and differential cytogenetic analyses involving C-banding, Ag-nucleolar organizer region (NOR) and chromomycin A3 (CMA3) staining, and FISH (fluorescent in situ hybridization) with 18S and 5S rDNA probes. Conorhynchus conirostris presents a higher diploid number (2n = 60) than those detected in Pimelodidae representatives, whereas Lophiosilurus alexandri, with a karyotype of 2n = 54 chromosomes, presents a chromosomal constitution similar to that found in the family Pseudopimelodidae. Plesiomorphic characteristics such as single NORs at terminal positions are found in both species, as revealed by CMA3 and silver nitrate staining, and FISH with a 18S rDNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Incertae sedis; Pimelodidae; Pseudopimelodidae; RDNA; Siluriformes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200008

Cytogenetics and DNA barcode reveal an undescribed Apareiodon species (Characiformes: Parodontidae)
Provedor de dados: 74
Autores: Santos,Emanoel Oliveira dos; Deon,Geize Aparecida; Almeida,Rafael Bonfim de; Oliveira,Ezequiel Aguiar de; Nogaroto,Viviane; Silva,Hugmar Pains da; Pavanelli,Carla Simone; Cestari,Marta Margarete; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando; Vicari,Marcelo Ricardo.
Abstract Parodontidae is a small group of fish and some species are particularly difficult to identify due to the lack of sufficiently consistent morphological traits. Cytogenetically, the species possess 2n = 54 chromosomes and are either sex-homomorphic or sex-heteromorphic (regarding its chromosomes). We evaluated data on color, tooth morphology, cytogenetics, and mitochondrial markers (COI) in Apareiodon specimens from the Aripuanã River (Amazon basin) and the results were compared to other congeneric taxa. Morphological results show an overlap of body color and tooth morphology to other known Apareiodon. The cytogenetics data showed that the 2n = 54 chromosomes, 50 m/sm + 4 st and, a ZZ/ZW sex chromosome system in Apareiodon sp. are common to other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosomal differentiation; Hidden diversity; Repetitive DNA; Sex chromosomes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300365

Comparative cytogenetics among species of the Astyanax scabripinnis complex: evolutionary and biogeographical inferences
Provedor de dados: 74
Autores: Vicari,Marcelo Ricardo; Noleto,Rafael Bueno; Artoni,Roberto Ferreira; Moreira-Filho,Orlando; Bertollo,Luiz Antonio Carlos.
Karyotype data are presented for distinct species of the genus Astyanax from four rivers belonging to three different hydrographic basins of the State of Paraná, Brazil (Verde River - Tibagi basin, Açungui River - Ribeira basin, and Santo Antônio and Jaguariaíva Rivers - Jaguariaíva basin). Three karyotypic forms were identified, here denominated karyotype A (2n = 50 chromosomes, with 8m+18sm+10st+14a, and thirteen 18S rDNA sites); karyotype B (2n = 50 chromosomes, with 8m+18sm+10st+1f4a, and four 18S rDNA sites); and karyotype C (2n = 48 chromosomes, with 10m+16sm+10st+12a, and eight 18S rDNA sites). The pattern of constitutive heterochromatin was similar among the three karyotypic forms, with few differences. The 5S rDNA corresponds to a synapomorphic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype evolution; Cytotaxonomy; RDNA sites; Biogeography.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200002

Comparative cytogenetics among populations of Astyanax altiparanae (Characiformes, Characidae, Incertae sedis)
Provedor de dados: 74
Autores: Ferreira Neto,Maressa; Vicari,Marcelo Ricardo; Camargo,Edemar Furquim de; Artoni,Roberto Ferreira; Moreira-Filho,Orlando.
Cytogenetic data are presented for Astyanax altiparanae populations from three Brazilian hydrographic systems. The chromosomal data obtained in A. altiparanae support the hypothesis of diploid number conservation. However, small differences in the karyotype formula and number of nucleolar organizer regions were observed in these populations. The apparent karyotypical similarity among the studied populations strongly suggests a close relationship among them with some chromosomal divergences due to gene flow restriction.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotaxonomy; Karyotype diversification; Fish cytogenetics.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400021

Cytotaxonomy of Parodon nasus and Parodon tortuosus (Pisces, Characiformes): A case of synonymy confirmed by cytogenetic analyses
Provedor de dados: 74
Autores: Bellafronte,Elisangela; Margarido,Vladimir Pavan; Moreira-Filho,Orlando.
Morphological and cytogenetical studies were carried out on the freshwater fish Parodon nasus and Parodon tortuosus in order to evaluate a putative synonymy. The diploid chromosome number observed in both species was 2n = 54 (48M/SM and 6ST) with no differences between the sexes. Despite slight differences in the pattern of heterochromatin distribution and the number of cusps in symphysean teeth, the location of nucleolar organizer regions (NORs) and 5S rRNA genes (both species-specific features) were similar in both species. The remarkable similarity observed between these allopatric species supports recent taxonomical reviews indicating that P. tortuosus is a synonym for P. nasus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Parodontidae; Systematics; 18S rDNA; 5S rDNA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500010

Satellite DNA sites in four species of the genus Astyanax (Teleostei, Characiformes)
Provedor de dados: 74
Autores: Kavalco,Karine Frehner; Pazza,Rubens; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando.
Cytogenetic data about satellite DNA distribution in four Astyanax species (Characidae) from the Paraitinga river, Paraíba do Sul river basin, Brazil, are presented. In order to characterize the constitutive heterochromatin, C-banding, chromomycin A3 and DAPI fluorescence staining, as well as fluorescence in situ hybridization (FISH) with the satellite DNA As-51 probe were performed. A. scabripinnis and A. parahybae presented 2n = 50 and 2n = 48 chromosomes, respectively. The heterochromatin was located in the pericentromeric and terminal regions of many chromosomes, corresponding to GC-positive regions and to the As-51 satellite DNA in terminal regions. A. intermedius and A. giton, both with 2n = 50 chromosomes, showed little heterochromatin, mostly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astyanax; Fluorescence in situ hybridization (FISH); Heterochromatin; Karyotypic diversity; Satellite DNA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400005

Contributions to the taxonomy of Trachelyopterus (Siluriformes): comparative cytogenetic analysis in three species of Auchenipteridae
Provedor de dados: 83
Autores: Santos,Dayane Petik dos; Felicetti,Denise; Baumgärtner,Lucas; Margarido,Vladimir Pavan; Blanco,Daniel Rodrigues; Moreira-Filho,Orlando; Lui,Roberto Laridondo.
ABSTRACT Auchenipteridae is divided into subfamilies Centromochlinae and Auchenipterinae. Parauchenipterus is included in the latter and is subject of taxonomic discussions concerning its validation or synonymization with Trachelyopterus. Herein, three species from two hydrographic basins were cytogenetically analyzed: Parauchenipterus striatulus from Doce River and two sympatric species, P. galeatus and Trachelyopterus coriaceus, from the Araguaia River. Diploid number of 58 chromosomes was verified for all species, but P. striatulus has different karyotype formula from the others. The three species have heterochromatin located in terminal regions of almost all chromosomes and in pericentromeric region on acrocentric chromosomes. Simple NORs was verified...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptic species; FISH; Parauchenipterus; Problematic taxonomy; 5S rDNA.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100214

Diversity and chromosomal evolution in the genusAncistrusKner, 1854 (Loricariidae: Ancistrini) from three hydrographic basins of Mato Grosso State, Brazil
Provedor de dados: 83
Autores: Mariotto,Sandra; Centofante,Liano; Moreira-Filho,Orlando.
Cytogenetic analyses were carried out in 117 specimens of seven species of the genus Ancistrus from three hydrographic in Mato Grosso State: Paraguay, Araguaia-Tocantins and Amazon basins. Conventional cytogenetic techniques were used to obtain mitotic chromosomes. C-banding was performed to detect heterochromatic regions and silver nitrate staining was used to identify nucleolar organizer regions (Ag-NORs). The counted and paired chromosomes revealed diploid numbers ranging from 2n = 40 to 2n = 54 with karyotype formulae varying from FN = 80 to FN = 86. Single marks in distinct chromosomes identified the nucleolar organizer regions. The constitutive heterochromatin was scarce in the diploid number from 2n = 50 to 2n = 54 and conspicuous blocks were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AgNOR; Cytogenetic; Diploid number; Heterochromatin.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100125

DNA barcode of Parodontidae species from the La Plata river basin - applying new data to clarify taxonomic problems
Provedor de dados: 83
Autores: Bellafronte,Elisangela; Mariguela,Tatiane Casagrande; Pereira,Luiz Henrique Garcia; Oliveira,Claudio; Moreira-Filho,Orlando.
In the past years, DNA barcoding has emerged as a quick, accurate and efficient tool to identify species. Considering the difficulty in identifying some Parodontidae species from the La Plata basin and the absence of molecular data for the group, we aimed to test the effectiveness of DNA barcoding and discuss the importance of using different approaches to solve taxonomic problems. Eight species were analyzed with partial sequences of Cytochrome c oxidase I. The mean intraspecific K2P genetic distance was 0.04% compared to 4.2% for mean interspecific K2P genetic distance. The analyses of distance showed two pairs of species with K2P genetic divergence lower than 2%, but enough to separate these species. Apareiodon sp. and A. ibitiensis, considered as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apareiodon; COI gene; Cytogenetics; Fish identification; Morphology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300497

Evidence of incipient speciation in Astyanax scabripinnis species complex (Teleostei: Characidae)
Provedor de dados: 83
Autores: Castro,Jonathan P.; Moura,Maurício O.; Moreira-Filho,Orlando; Shibatta,Oscar A.; Santos,Mateus H.; Nogaroto,Viviane; Vicari,Marcelo R.; Almeida,Mara C. de; Artoni,Roberto F.
Two populations of the Astyanax scabripinniscomplex, isolated by a waterfall with over 100 meters depth and inhabiting different altitudes of the same river (1850 m a.s.l. and 662 m a.s.l.) were compared in reproductive data, geometric morphometry, tooth morphology, anal-fin rays counts, and karyotype, in order to test the hypothesis of speciation between the two populations. The results in the geometric morphometry analysis showed differences between the populations. Discriminant function analysis (DFA) and canonical variance analysis revealed sexual dimorphism. Secondary sexual characters, such as hooks in the anal fin rays of the males are absent in the lower altitude population. Both populations had the same macro karyotype structure, except for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allopatric populations; Cytogenetics; Evolution; Geometric morphometry; Paraíba do Sul River.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000200429

Comparative cytogenetic analyses in Ancistrus species (Siluriformes: Loricariidae)
Provedor de dados: 83
Autores: Glugoski,Larissa; Deon,Geize; Schott,Stephane; Vicari,Marcelo R.; Nogaroto,Viviane; Moreira-Filho,Orlando.
ABSTRACT Ancistrus is a specious genus of armored catfishes that has been extensively used for cytogenetic studies in the last 17 years. A comparison of the extensive karyotypic plasticity within this genus is presented with new cytogenetic analysis for Ancistrus cf. multispinis and Ancistrus aguaboensis. This study aims to improve our understanding of chromosomal evolution associated with changes in the diploid number (2n) and the dispersion of ribosomal DNAs (rDNAs) within Ancistrus. Ancistrus cf. multispinis and A. aguaboensis exhibit 2n of 52 and 50 chromosomes, respectively. Given that A. cf. multispinis shares a 2n = 52 also found in Pterygoplichthyini, the sister group for Ancistrini, a Robertsonian (Rb) fusion event is proposed for the 2n reduction...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Armored catfish; FISH; 5S rDNA; 18S rDNA; Telomeric sequence.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000200217

Microstructural chromosome reorganization in the genus Trichomycterus (Siluriformes: Trichomycteridae)
Provedor de dados: 83
Autores: Oliveira,Maria Lígia M. de; Utsunomia,Ricardo; Pansonato-Alves,José Carlos; Scacchetti,Priscilla C.; Primo,Cleberson C.; Vicari,Marcelo R.; Artoni,Roberto F.; Centofante,Liano; Moreira-Filho,Orlando; Oliveira,Claudio; Foresti,Fausto.
Trichomycterus is a specious fish genus within Trichomycterinae and displays remarkable karyotype diversity. However, knowledge about their genomic structure and location of repetitive sequence is still limited. In order to better understand the karyotype diversification, we analyzed nine species of Trichomycterus using classical and molecular cytogenetic techniques. Results revealed a conserved diploid chromosome number of 2n=54 chromosomes in all analyzed species, although remarkable differences on the constitutive heterochromatin distribution were observed. In addition, while the 18S rDNA showed a conserved distribution pattern, the 5S rDNA sites showed a quite diverse location considering the analyzed species. Remarkably, both ribosomal genes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype diversity; Repetitive DNA; RDNA; Supernumerary chromosomes.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200211

Cytogenetics of Imparfinis schubarti (Siluriformes: Heptapteridae) from the Piumhi drainage, a diverted river in Minas Gerais State, Brazil
Provedor de dados: 103
Autores: Kantek,Daniel L. Z.; Peres,Wellington A. M.; Buckup,Paulo A.; Moreira-Filho,Orlando.
Specimens of Imparfinis schubarti (Gomes, 1956) collected in the Piumhi river drainage, state of Minas Gerais, Brazil, were studied cytogenetically. The river was diverted from the Rio Grande Basin into the São Francisco basin in the early 1960s. All individuals presented 2n = 58 chromosomes, including 18 metacentric, 34 submetacentric and six subtelocentric chromosomes. A secondary constriction was observed in the interstitial region of the long arm of the largest chromosome pair, coinciding with the NOR. A single conspicuous heterochromatic block located in the largest pair of metacentric chromosomes was observed, adjacent to the secondary constriction. A detectable 18S rDNA probe hybridization region occurs in only one chromosome pair and is synthenic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transposed river; Subclade nemuroglanis; FISH; São Francisco River Basin.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702009000400018

Chromosomal differentiation between populations of Oligosarcus hepsetus (Teleostei, Characidae) from small tributaries at opposite margins of the Paraíba do Sul river (Brazil)
Provedor de dados: 52
Autores: Centofante,Liano; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando.
Cytogenetic analyses were carried out in two populations of Oligosarcus hepsetus from tributaries at opposite margins of the Paraíba do Sul river. The same diploid number was observed in both populations (2n=50), but they showed remarkable differences related to karyotype formula and distribution of rDNA sites as revealed by silver nitrate staining and in situ hybridization with 18S probes. The results suggested that the main channel of the Paraíba do Sul river acted as barrier to gene flow between populations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Headstream; Cytogenetic; Differentiation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700016