Consulta: creatorFacets:"Mossong, Joël"
Registros recuperados: 2
Data/hora: 15/06/2024 09:28:53

Campylobacter armoricus sp. nov., a novel member of the Campylobacter lari group isolated from surface water and stools from humans with enteric infection
Provedor de dados: 5
Autores: Boukerb, Amine M.; Penny, Christian; Serghine, Joelle; Walczak, Cécile; Cauchie, Henry-michel; Miller, William G.; Losch, Serge; Ragimbeau, Catherine; Mossong, Joël; Mégraud, Francis; Lehours, Philippe; Bénéjat, Lucie; Gourmelon, Michele.
During a study on the prevalence and diversity of members of the genus Campylobacter in a shellfish-harvesting area and its catchment in Brittany, France, six urease-positive isolates of members of the genus Campylobacter were recovered from surface water samples, as well as three isolates from stools of humans displaying enteric infection in the same period. These strains were initially identified as members of the Campylobacter lari group by MALDI-TOF mass spectrometry and placed into a distinct group in the genus Campylobacter, following atpA gene sequence analysis based on whole-genome sequencing data. This taxonomic position was confirmed by phylogenetic analysis of the 16S rRNA, rpoB and hsp60 (groEL) loci, and an analysis of the core genome that...
Tipo: Text
Palavras-chave: Campylobacter; Novel species; Surface water; Coastal catchment; Human gastroenteritis; C. lari group; Whole genorne sequence.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00591/70346/69558.pdf

Metagenomics-Based Proficiency Test of Smoked Salmon Spiked with a Mock Community
Provedor de dados: 5
Autores: Sala, Claudia; Mordhorst, Hanne; Grützke, Joesphine; Brinkmann, Annika; Petersen, Thomas N; Poulsen, Casper; Cotter, Paul D; Crispie, Fiona; Ellis, Richard J; Castellani, Gastone; Amid, Clara; Hakhverdyan, Mikhayil; Le Guyader, Soizick; Manfreda, Gerardo; Mossong, Joël; Nitsche, Andreas; Ragimbeau, Catherine; Schaeffer, Julien; Schlundt, Joergen; Tay, Moon Y. F.; Aarestrup, Frank M.; Hendriksen, Rene S.; Pamp, Sünje Johanna; De Cesare, Alessandra.
An inter-laboratory proficiency test was organized to assess the ability of participants to perform shotgun metagenomic sequencing of cold smoked salmon, experimentally spiked with a mock community composed of six bacteria, one parasite, one yeast, one DNA, and two RNA viruses. Each participant applied its in-house wet-lab workflow(s) to obtain the metagenomic dataset(s), which were then collected and analyzed using MG-RAST. A total of 27 datasets were analyzed. Sample pre-processing, DNA extraction protocol, library preparation kit, and sequencing platform, influenced the abundance of specific microorganisms of the mock community. Our results highlight that despite differences in wet-lab protocols, the reads corresponding to the mock community members...
Tipo: Text
Palavras-chave: Shotgun metagenomics; Smoked salmon; Proficiency test; Experimentally spiked samples; Wet-lab protocols.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00664/77615/79636.pdf