Consulta: creatorFacets:"Motoike,Sérgio Yoshimitsu"
Registros recuperados: 14
Data/hora: 10/06/2024 09:17:14

Genetic diversity in table grapes based on RAPD and microsatellite markers
Provedor de dados: 86
Autores: Leão,Patrícia Coelho de Souza; Motoike,Sérgio Yoshimitsu.
The objective of this work was to analyze the genetic diversity of 47 table grape accessions, from the grapevine germplasm bank of Embrapa Semiárido, using 20 RAPD and seven microsatellite markers. Genetic distances between pairs of accessions were obtained based on Jaccard's similarity index for RAPD data and on the arithmetic complement of the weighted index for microsatellite data. The groups were formed according to the Tocher's cluster analysis and to the unweighted pair‑group method with arithmetic mean (UPGMA). The microsatellite markers were more efficient than the RAPD ones in the identification of genetic relationships. Information on the genetic distance, based on molecular characteristics and coupled with the cultivar agronomic performance,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitis vinifera; Cluster analysis; Genetic divergence; Grapevine; Multivariate analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000900010

Effect of Nitrogen and Potassium Rates on Early Development of Macaw Palm
Provedor de dados: 90
Autores: Pimentel,Leonardo Duarte; Bruckner,Claudio Horst; Martinez,Hermínia Emília Prieto; Motoike,Sérgio Yoshimitsu; Manfio,Candida Elisa; Santos,Rafael Carlos dos.
ABSTRACT The economic exploitation of macaw palm [Acrocomia aculeate(Jacq.) Lodd. ex Mart.] is currently in transition, from extractivism to agricultural cultivation, thus requiring studies on the fertilization of the crop. This study evaluated the response of three genotypes of macaw palm to increasing rates of nitrogen and potassium, grown in the field until the 2nd year and to establish reference contents of mineral nutrients in the leaf. The experiment was a split-plot randomized block design with five main treatments (N and K rates) and three secondary treatments (genotypes), with three replications, each plot containing three plants. Plant height, leaf number, vigor, and nutrient contents in leaf tissues were evaluated at the end of 2nd year of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acrocomia aculeata; Side-dressing fertilization; Macaw palm; Leaf mineral nutrient content.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000601671

Induction of somatic embryogenesis in immature seeds of guavatree cv. Paluma
Provedor de dados: 94
Autores: Moura,Elisa Ferreira; Motoike,Sérgio Yoshimitsu.
The biotechnological techniques may help solve many problems of guava culture, such as the high perishability of fruits. Somatic embryogenesis can generate highly multiplicative cell cultures and with high regenerative potential, serving as basis for genetic transformation. The aim of this work was to obtain somatic embryogenesis of guava (Psidium guajava L.) cv. Paluma. Immature seeds were used, and they were inoculated in MS environment containing 400 mg L-1 of L-glutamine, 100 mg L-1 myo-inositol, 60 g L-1 sucrose, 100 mg L-1 ascorbic acid and supplemented with different types and concentrations of growth regulators. Embryogenic callus appeared after 37 days of culture in an environment containing 1.0 mg L-1 2.4-D + 2.0 mg L-1 2-ip, in 7% of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tissue culture; Growth regulators; Myrtaceae.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452009000200027

Diversity and genetic relatedness among genotypes of Vitis spp. using microsatellite molecular markers
Provedor de dados: 94
Autores: Leão,Patrícia Coelho de Souza; Cruz,Cosme Damião; Motoike,Sérgio Yoshimitsu.
The purpose of this research was to study the genetic diversity and genetic relatedness of 60 genotypes of grapevines derived from the Germplasm Bank of Embrapa Semiárido, Juazeiro, BA, Brazil. Seven previously characterized microsatellite markers were used: VVS2, VVMD5, VVMD7, VVMD27, VVMD3, ssrVrZAG79 and ssrVrZAG62. The expected heterozygosity (He) and polymorphic information content (PIC) were calculated, and the cluster analysis were processed to generate a dendrogram using the algorithm UPGMA. The He ranged from 81.8% to 88.1%, with a mean of 84.8%. The loci VrZAG79 and VVMD7 were the most informative, with a PIC of 87 and 86%, respectively, while VrZAG62 was the least informative, with a PIC value of 80%. Cluster analysis by UPGMA method allowed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grape; Multivariate analysis; Genealogy; SSR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452013000300017

Genetic diversity of table grape based on morphoagronomic traits
Provedor de dados: 63
Autores: Leão,Patrícia Coelho de Souza; Cruz,Cosme Damião; Motoike,Sérgio Yoshimitsu.
The conservation and characterization of grape (Vitis spp) genetic resources in germplasm banks have been the basis of its use in breeding programs that result in development of new cultivars. There are at least 10,000 grape cultivars kept in germplasm collection. The genetic diversity in 136 table grape accessions from the state of Bahia, Brazil, was evaluated. Continuous and discrete morphoagronomic traits were assessed. The clustering analysis by the Tocher otimization method resulted in 30 clusters (considering continuous morphoagronomic traits), and 9 clusters (taking into consideration multicategorical traits). There was no agreement between clusters obtained by both, continuous or discrete phenotypic descriptors, independent of the cluster method...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitis spp; Genetic resources; Grape; Multivariate analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000100007

Anatomy, histochemistry and ultrastructure of seed and somatic embryo of Acrocomia aculeata (Arecaceae)
Provedor de dados: 63
Autores: Moura,Elisa Ferreira; Ventrella,Marília Contin; Motoike,Sérgio Yoshimitsu.
Macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Mart.) is a tropical species with multiple uses, including oil supply for biodiesel production. However, structural and physiological studies of the seed are still scarce, as well as in vitro propagation techniques. The aim of this study was to characterize the anatomy, histochemistry and ultrastructure of the seed and the in vitro somatic embryo of A. aculeata, gaining insight into relationships between the post-harvest seed behavior and the somatic embryo conversion to plant. The zygotic embryo and the endosperm show high quantities of protein and lipids, stored in protein and lipid bodies, respectively. The cell wall storage polysaccharides occur in the thickened cell wall of the endosperm. The absence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macaw palm; Zygotic embryo; Endosperm; Storage reserves.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000400004

Enzyme activity and reserve mobilization during Macaw palm ( Acrocomia aculeata ) seed germination
Provedor de dados: 39
Autores: Bicalho,Elisa Monteze; Motoike,Sérgio Yoshimitsu; Lima e Borges,Eduardo Euclydes de; Ataíde,Glauciana da Mata; Guimarães,Valéria Monteze.
ABSTRACT Reserve mobilization in seeds occurs after visible germination, which is marked by the protrusion of the radicle or cotyledonary petiole, as in species of Arecaceae. Acrocomia aculeata (macaw palm), usually produces hard seeds whose endosperm has mannan-rich cell walls. We investigated the composition of storage compounds in macaw palm seed and the roles of two enzymes (endo-β-mannanase, α-galactosidase) during and after germination. The seeds were firstly submitted to pre-established protocol to overcome dormancy and promote germination. Enzyme activity in both embryo and endosperm were assayed from the initiation of germinative activities until leaf sheath appearance, and the status of seed structures and reserve compounds were evaluated....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-galactosidase; Arecaceae; Endo-β-mannanase; Galactomannan; Lipid; Palm seed; Protein; Starch.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000300438

Storage on the vigor and viability of macauba seeds from two provenances of Minas Gerais State
Provedor de dados: 65
Autores: Souza,Patrícia Pereira de; Motoike,Sérgio Yoshimitsu; Carvalho,Mychelle; Kuki,Kacilda Naomi; Borges,Eduardo Euclydes de Lima e; Silva,Antônio Marcos.
ABSTRACT: Macauba palm stands out for having favorable features to biodiesel production such as the high oil content of its fruit. Considering the great potential of the species and their applicability in the renewable energy field, it becomes indispensable to establish the right conditions for storing the seeds for propagation purpose. The aim of this research was to evaluate the effect of seed moisture content, packaging, and storage conditions such as temperature and relative humidity on the quality of seeds from Minas Gerais State, during a 12-month storage period. The research had two independent assays: (I) the seeds were stored with three moisture contents/ranges 4.0≤6.0%; 6.0≤8.0% and 8.0≤10.0% in impermeable packages, under room temperature and at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acrocomia aculeata; Recalcitrant; Orthodox.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001101932

Propagação in vitro de genótipos de alface via embriogênese somática
Provedor de dados: 65
Autores: Pinheiro,Marcos Vinícius Marques; Silva,Thaís Cristina Ribeiro da; Maia,Ciro; Lima,Brenda Ventura; Motoike,Sérgio Yoshimitsu.
A propagação in vitro via embriogênese somática é uma alternativa eficiente para a propagação em larga escala do material vegetal. No entanto, não há relatos do desenvolvimento de protocolos completos de embriogênese somática, com produção de plantas de alface. O presente trabalho teve como objetivo estabelecer a propagação in vitro de genótipos de alface, Paris White e Red Salad Bowl, avaliando a indução dos calos embriogênicos, regeneração dos embriões somáticos e posterior conversão em plantas. Para a indução de embriogênese somática, duas fontes de explante (folhas cotiledonares inteiras e seccionadas) foram cultivadas em meio MS+10,75µM de ANA+0,89µM de BA. A proliferação dos calos embriogênicos foi realizada em meio MS+24µM de AIA+0,15µM de BA. Para...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactuca sativa; Embrião somático; Micropropagação.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012001100007

Early selection of elite clones of an ornamental bromeliad in vitro
Provedor de dados: 65
Autores: Manfio,Candida Elisa; Motoike,Sérgio Yoshimitsu; Paula,Cláudio Coelho de; Valente,Magno Sávio; Melo,Cristiane Gamarano.
Orthophytum grossiorum is a typical bromeliad from Atlantic forestry threatened of extinction. The objectives of this research were to select O. grossiorum clones with ornamental values easy to propagate in vitro, and establish in vitro propagation protocols for these clones. The project was developed in three steps: germination and in vitro selection of seedlings responsive to BAP (6-benzylaminopurine), selection of clones with ornamental values, and establishment of protocol for in vitro propagation of the selected clones. In the first step only 18.33% of plantlets germinated in vitro were responsive to BAP. These plantlets were selected and replicated in vitro several times, each replicated plantlet constituting a clone. In the second step these clones...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micro-propagation; Tissue culture; Breeding.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000700009

Repetibilidade em características biométricas do fruto de macaúba
Provedor de dados: 65
Autores: Manfio,Candida Elisa; Motoike,Sérgio Yoshimitsu; Santos,Carlos Eduardo Magalhães dos; Pimentel,Leonardo Duarte; Queiroz,Vanessa de; Sato,Aurora Yoshiko.
Estimativas do coeficiente de repetibilidade têm sido utilizadas no estudo de caracteres de várias espécies de palmeiras, auxiliando na definição do número e período adequado de avaliações dos genótipos para maior eficiência dos programas de melhoramento. Para macaúba, não há relatos de estudos dessa natureza. Nesse contexto, faz-se importante a determinação do coeficiente de repetibilidade das seguintes características biométricas dos frutos: peso do fruto, volume do fruto, diâmetro equatorial do fruto, peso da amêndoa, volume da amêndoa, diâmetro da amêndoa e espessura do endocarpo; e a determinação do número mínimo de avaliações para um eficiente processo de seleção e avaliação de subamostras de macaúba. O trabalho foi desenvolvido no Departamento de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acrocomia aculeata; Medições; Estimação.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000100012

Manejo da irrigação e fertirrigação com nitrogênio sobre as características químicas da videira 'Niágara Rosada'
Provedor de dados: 65
Autores: Busato,Cristiani Campos Martins; Soares,Antonio Alves; Sediyama,Gilberto Chohaku; Motoike,Sérgio Yoshimitsu; Reis,Edvaldo Fialho dos.
A qualidade da uva pode responder ao manejo da irrigação e ao ajuste da adubação nitrogenada. Assim, este trabalho teve como objetivo estudar o efeito do manejo de irrigação e doses de nitrogênio sobre a qualidade da uva 'Niagara Rosada' no município de Colatina-ES. Foram avaliadas três doses de nitrogênio, aplicadas via fertirrigação: 35, 70 e 140g planta-1 e quatro estratégias de manejo de irrigação. No início do experimento até a fase de maturação das bagas, todos os tratamentos receberam a mesma lâmina de irrigação, elevando-se a umidade do solo à capacidade de campo (M1=100%). Após este período, foram avaliados mais três estratégias de manejo de irrigação: m²=67%, M3=33% e M4=0% da lâmina correspondente ao tratamento M1. Foram avaliados os teores de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitis; Nutrição mineral; Estresse hídrico.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000700013

Propagação in vitro de genótipos de alface via embriogênese somática
Provedor de dados: 65
Autores: Pinheiro,Marcos Vinícius Marques; Silva,Thaís Cristina Ribeiro da; Maia,Ciro; Lima,Brenda Ventura; Motoike,Sérgio Yoshimitsu.
Tipo: Info:eu-repo/semantics/other
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100031

Efeito da uréia no alongamento e enraizamento de microplantas de bananeira in vitro
Provedor de dados: 64
Autores: Ramos,Rachel Soares; Motoike,Sérgio Yoshimitsu; Moura,Elisa Ferreira; Gomes,Simone Bhering de Souza; Rodrigues,Vânia de Fátima; Oliveira,Márcio Antonio Rocha de.
A micropropagação de bananeira (Musa spp.) via ápices caulinares já está bem estabelecida para diversas cultivares. Entretanto, podem ser feitos aperfeiçoamentos nos protocolos visando a obtenção de mudas mais vigorosas. Objetivou-se, neste trabalho, verificar a adição de nitrogênio na forma de uréia em diferentes concentrações (0, 100, 200, 400 e 800 mg L-1), no alongamento e enraizamento de brotos das cultivares Grande Naine, Prata Anã, Maçã e Nanicão. Houve efeito positivo da uréia no crescimento dos explantes, na produção de raízes e de massa seca para a cultivar Maçã, até 200 mg L-1 de uréia. As cultivares Prata Anã e Grande Naine não foram beneficiadas com a adição de uréia, e a concentração de 800 mg L-1 gerou morte das microplantas dessas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Musa spp.; Cultura de tecidos; Fonte de nitrogênio.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000700024