Consulta: creatorFacets:"Nagata,Tatsuya"
Registros recuperados: 11
Data/hora: 06/06/2024 17:00:18

Diferenciação de estirpes de Potato virus Y (PVY) por RT-PCR
Provedor de dados: 75
Autores: Fonseca,Leonardo N.; Inoue-Nagata,Alice K.; Nagata,Tatsuya; Singh,Rudra P.; Ávila,Antonio Carlos de.
O Potato virus Y (PVY) tornou-se o maior problema nas áreas de produção de batata semente do Brasil. Somente as estirpes comum (PVYº) e necrótica (PVY N) eram detectadas infectando batata no Brasil. Esta situação mudou drasticamente a partir de 1997 quando um surto epidêmico de uma variante da estirpe necrótica de PVY causando arcos e anéis necróticos na superfície do tubérculo (PVY NTN) foi observado no país. Este estudo visou avaliar e validar uma metodologia de diferenciação de estirpes que causam necrose em tubérculos, proposta por Weilguny e Singh (1998). Vinte e oito isolados de PVY originários de tubérculos infectados de batata, provenientes de quatro estados brasileiros, foram analisados em sua reação em plantas de fumo, por Elisa, utilizando...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum tuberosum; Vírus Y da batata; Reação em cadeia da polimerase; Potyvírus.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362005000400008

Detecção por sorologia do Melon yellowing associated virus (MYaV) em áreas produtoras de melão no Nordeste brasileiro
Provedor de dados: 75
Autores: Lima,Mirtes F; Nagata,Tatsuya; Neves,Filipe M; Inoue-Nagata,Alice K; Moita,Antônio W; Sousa,Carla; Vecchia,Marília Della; Rangel,Maurício G; Dias,Rita de CS; Dutra,Luiza S; Ávila,Antônio C de.
O Nordeste é a principal região produtora de melão do País, contribuindo com mais de 90% da produção nacional. Entretanto, o "amarelão do meloeiro", doença associada à presença de um novo vírus, o Melon yellowing-associated virus (MYaV), vem afetando a cultura desde 1999. Este trabalho teve como objetivo detectar a ocorrência do MYaV em meloeiros exibindo sintomas suspeitos da doença nos estados que são os maiores produtores da Região Nordeste. Em novembro de 2007, plantios comerciais de melão foram inspecionados para avaliar a ocorrência do "amarelão". Um total de 374 plantas foi coletado em áreas produtoras dos estados do RN (54) e CE (37) e também, do Submédio do Vale do São Francisco (283), situado nos Estados da BA e PE. A detecção do MYaV nas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Detecção; Sorologia; DAS-Elisa; Amarelão; Levantamento.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362009000400013

A reliable begomovirus inoculation method for screening Lycopersicon esculentum lines
Provedor de dados: 75
Autores: Inoue-Nagata,Alice K; Nagata,Tatsuya; Ávila,Antonio C de; Giordano,Leonardo de B.
Due to failures of infection after mechanical inoculation of begomovirus on tomato (Lycopersicon esculentum Mill.) plants, an efficient and reliable begomovirus inoculation method was developed using the whitefly vector (Bemisia tabaci). Virus acquisition was carried out using a 'Tube-cage', made of a polypropylene tube, containing one to two day-old whiteflies and a tomato plant apex infected with a begomovirus isolate. After 48 h of acquisition access period, inoculation was done using a 'Ring-cage', containing viruliferous whiteflies, attached to the abaxial side of the leaf of young tomato plants. 48 hours after acquisition, the whiteflies were eliminated and the inoculated plants were incubated for 28 days under greenhouse conditions. Three...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lycopersicon esculentum; Inoculation; Resistance; Screening; Geminivirus.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362007000300024

Detection of three Allexivirus species infecting garlic in Brazil
Provedor de dados: 86
Autores: Melo Filho,Péricles de Albuquerque; Nagata,Tatsuya; Dusi,André Nepomuceno; Buso,José Amauri; Torres,Antonio Carlos; Eiras,Marcelo; Resende,Renato de Oliveira.
Garlic viruses often occur in mixed infections under field conditions. In this study, garlic samples collected in three geographical areas of Brazil were tested by Dot-ELISA for the detection of allexiviruses using monoclonal specific antibodies to detect Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and a polyclonal antiserum able to detect the three virus species mentioned plus Garlic virus D (GarV-D). The detected viruses were biologically isolated by successive passages through Chenopodium quinoa. Reverse Transcriptase Polimerase Chain Reaction (RT-PCR) was performed using primers designed from specific regions of the coat protein genes of Japanese allexiviruses available in the Genetic Bank of National Center of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium sativum; Virus detection; Coat protein; Dot-ELISA; RT-PCR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000800002

Distinct features of Pepper yellow mosaic virus isolates from tomato and sweetpepper
Provedor de dados: 102
Autores: Cunha,Luis C. V. da; Resende,Renato de O.; Nagata,Tatsuya; Inoue-Nagata,Alice K.
Determination of virus diversity in the field is vital to support a sustainable breeding program for virus resistance of horticultural crops. The present study aimed to characterize four field potyvirus isolates found naturally infecting sweet pepper (Capsicum annuum) (Sa66 and Sa115) and tomato (Lycopersicon esculentum) (IAC3 and Sa21) plants. Their biological characteristics revealed differences among the isolates in their ability to infect distinct Capsicum spp. and tomato genotypes, and in the severity of symptoms caused by these isolates compared to the infection caused by an isolate of Pepper yellow mosaic virus (PepYMV). Absence of cross-reaction was found among the studied isolates with antiserum against Potato virus Y (PVY). However, all isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PepYMV; Potyvirus; Lycopersicon esculentum; Capsicum annuum.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000600012

Print-capture PCR for detection of tomato begomoviruses from plants and whiteflies
Provedor de dados: 102
Autores: Nagata,Tatsuya; Inoue-Nagata,Alice K; Ávila,Antonio C. de; Giordano,Leonardo de B.
Print-capture (PC) Polymerase chain reaction (PCR) was evaluated as a novel detection method of plant viruses. Tomato (Lycopersicon esculentum) plants infected with begomovirus (fam. Geminiviridae, gen. Begomovirus) and viruliferous whiteflies were used to study the efficiency of the method. Print-capturing steps were carried out using non-charged nylon membrane or filter paper as the solid support for DNA printings. Amplified DNA fragments of expected size were consistently obtained by PCR from infected plants grown in a greenhouse, after direct application of printed materials to the PCR mix. However, virus detection from a single whitefly and from field-grown tomato samples required a high temperature treatment of printed material prior to PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Geminivírus; Bemisia tabaci; Bemisia argentifoli.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000100014

The fluctuation of transmission specificity and efficiency of Tomato spotted wilt virus by Frankliniella schultzei
Provedor de dados: 102
Autores: Nagata,Tatsuya; Resende,Renato O.; Inoue-Nagata,Alice K.; Ávila,Antonio Carlos de.
Tipo: Info:eu-repo/semantics/report
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000500012

Identification and rearing of four thrips species vectors of Tospovirus in the Federal District, Brazil
Provedor de dados: 44
Autores: Nagata,Tatsuya; Mound,Laurence A.; França,Felix H.; Ávila,Antonio C. de.
The disease caused by the viruses belonging to the genus Tospovirus is a serious problem in horticultural and ornamental crops in Brazil. In the field, this virus group is transmitted by the insect vector, thrips. Little is known about the thrips species occurring in Brazil, and which species are important for the dissemination of these diseases. For the study of epidemiology of this virus group, different thrips populations, which may have the capacity to be vectors of tospoviruses, were collected in the Federal District. Four species were identified: Frankliniella occidentalis Pergande, F. schultzei Trybom, Thrips palmi Karny and T. tabaci Lindeman. This is the first report of the occurrence of F. occidentalis and T. palmi in the Federal District. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Thysanoptera; Spotted wilt; TSWV; Western flower thrips.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591999000300022

Serological Identification of Virus in Watermelon Production Fields in the Tocantins State
Provedor de dados: 52
Autores: Aguiar,Raimundo Wagner de Souza; Rodrigues,Adelmo; Portella,Augustus Caeser Franke; Lopes,Magnolia de Mendonça; Lima,Mirtes Freitas; Resende,Renato de Oliveira; Nagata,Tatsuya.
Watermelon (Citrullus lanatus) cultivated in almost all tropical and subtropical regions of the world, has its largest output in China, and then, according to FAO data, Turkey, Iran and Brazil, being one of the main crops cultivated in State of Tocantins, Brazil. In this work was investigated the occurrence and distribution of the watermelon viruses, totaling 752 samples taken in a stratified experimental design in four representative regions of production: Gurupi (150), Lagoa da Confusao (232), Formoso do Araguaia (265) and Porto Nacional (105). The sampling and collecting the leaves of plants with the presence of symptoms were performed once a week during the entire cultivation cycle. As a result, were observed by Dot-ELISA method, different types of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Watermelon; Citrullus lanatus; Potyvirus; Cucumovirus; Tospovirus; Serological assay.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200192

Production of Brazilian human norovirus VLPs and comparison of purification methods
Provedor de dados: 58
Autores: Lamounier,Thais Alves da Costa; Oliveira,Layssa Miranda de; Camargo,Brenda Rabello de; Rodrigues,Kelly Barreto; Noronha,Eliane Ferreira; Ribeiro,Bergmann Morais; Nagata,Tatsuya.
Abstract Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Norwalk virus; VLP; Ion-exchange chromatography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265

Detecção de um begomovírus em amostras foliares de tomateiro com sondas não-radioativas
Provedor de dados: 65
Autores: Santana,Flávio Martins; Inoue-Nagata,Alice Kazuko; Nagata,Tatsuya; Ribeiro,Simone da Graça; Ávila,Antônio Carlos de; Giordano,Leonardo de Britto.
O aumento na ocorrência de begomoviroses (geminiviroses) em tomateiros, Lycopersicon esculentum Mill., em várias regiões brasileiras, vem causando grandes prejuízos para o agronegócio de tomate, devido à ocorrência do inseto vetor, Bemisia argentifolii (Bemisia tabaci biotipo B). A diagnose é realizada em geral por "polymerase chain reaction" (PCR) ou por hibridização com sondas radioativas. A PCR é um método de alta sensibilidade, porém apresenta a desvantagem da possibilidade de obtenção de resultados falso-positivos, devido a contaminações, ou falso-negativos causados por inibidores contaminantes da reação, ou pela extrema especificidade dos iniciadores. A hibridização de ácidos nucléicos com sondas radioativas tem o seu uso limitado devido à...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lycopersicon esculentum; Geminivírus; Diagnose; Digoxigenina.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000100044