Consulta: creatorFacets:"Netto,C.A."
Registros recuperados: 7
Data/hora: 16/06/2024 19:21:33

Viability of mesenchymal stem cells during electrospinning
Provedor de dados: 56
Autores: Zanatta,G.; Steffens,D.; Braghirolli,D.I.; Fernandes,R.A.; Netto,C.A.; Pranke,P.
Tissue engineering is a technique by which a live tissue can be re-constructed and one of its main goals is to associate cells with biomaterials. Electrospinning is a technique that facilitates the production of nanofibers and is commonly used to develop fibrous scaffolds to be used in tissue engineering. In the present study, a different approach for cell incorporation into fibrous scaffolds was tested. Mesenchymal stem cells were extracted from the wall of the umbilical cord and mononuclear cells from umbilical cord blood. Cells were re-suspended in a 10% polyvinyl alcohol solution and subjected to electrospinning for 30 min under a voltage of 21 kV. Cell viability was assessed before and after the procedure by exclusion of dead cells using trypan blue...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanotechnology; Tissue engineering; Electrospinning; Stem cells.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200006

Effect of skilled and unskilled training on nerve regeneration and functional recovery
Provedor de dados: 56
Autores: Pagnussat,A.S.; Michaelsen,S.M.; Achaval,M.; Ilha,J.; Hermel,E.E.S.; Back,F.P.; Netto,C.A.
The most disabling aspect of human peripheral nerve injuries, the majority of which affect the upper limbs, is the loss of skilled hand movements. Activity-induced morphological and electrophysiological remodeling of the neuromuscular junction has been shown to influence nerve repair and functional recovery. In the current study, we determined the effects of two different treatments on the functional and morphological recovery after median and ulnar nerve injury. Adult Wistar male rats weighing 280 to 330 g at the time of surgery (N = 8-10 animals/group) were submitted to nerve crush and 1 week later began a 3-week course of motor rehabilitation involving either "skilled" (reaching for small food pellets) or "unskilled" (walking on a motorized treadmill)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peripheral nerve injury; Functional recovery; Median nerve; Ulnar nerve; Nerve morphometry.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800010

Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury
Provedor de dados: 56
Autores: Nicola,F.C.; Rodrigues,L.P.; Crestani,T.; Quintiliano,K.; Sanches,E.F.; Willborn,S.; Aristimunha,D.; Boisserand,L.; Pranke,P.; Netto,C.A.
Spinal cord injury (SCI) is a disabling condition resulting in deficits of sensory and motor functions, and has no effective treatment. Considering that protocols with stem cell transplantation and treadmill training have shown promising results, the present study evaluated the effectiveness of stem cells from human exfoliated deciduous teeth (SHEDs) transplantation combined with treadmill training in rats with experimental spinal cord injury. Fifty-four Wistar rats were spinalized using NYU impactor. The rats were randomly distributed into 5 groups: Sham (laminectomy with no SCI, n=10); SCI (laminectomy followed by SCI, n=12); SHEDs (SCI treated with SHEDs, n=11); TT (SCI treated with treadmill training, n=11); SHEDs+TT (SCI treated with SHEDs and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spinal cord injury; Stem cells; Treadmill training; Functional recovery; MASCIS impactor.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900604

Ischemic preconditioning reduces peripheral oxidative damage associated with brain ischemia in rats
Provedor de dados: 56
Autores: Frassetto,S.S.; Schetinger,M.R.C.; Webber,A.; Sarkis,J.J.F.; Netto,C.A.
Brain ischemia followed by reperfusion causes neuronal death related to oxidative damage. Furthermore, it has been reported that subjects suffering from ischemic cerebrovascular disorders exhibit changes in circulating platelet aggregation, a characteristic that might be important for their clinical outcome. In the present investigation we studied tert-butyl hydroperoxide-initiated plasma chemiluminescence and thiol content as measures of peripheral oxidative damage in naive and preconditioned rats submitted to forebrain ischemia produced by the 4-vessel occlusion method. Rats were submitted to 2 or 10 min of global transient forebrain ischemia followed by 60 min or 1, 2, 5, 10 or 30 days of reperfusion. Preconditioned rats were submitted to a 10-min...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain ischemia; Ischemic preconditioning; Oxidative damage; Hydroperoxide-initiated chemiluminescence; Plasma thiols.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000017

Transplantation of mononuclear cells from human umbilical cord blood promotes functional recovery after traumatic spinal cord injury in Wistar rats
Provedor de dados: 56
Autores: Rodrigues,L.P.; Iglesias,D.; Nicola,F.C.; Steffens,D.; Valentim,L.; Witczak,A.; Zanatta,G.; Achaval,M.; Pranke,P.; Netto,C.A.
Cell transplantation is a promising experimental treatment for spinal cord injury. The aim of the present study was to evaluate the efficacy of mononuclear cells from human umbilical cord blood in promoting functional recovery when transplanted after a contusion spinal cord injury. Female Wistar rats (12 weeks old) were submitted to spinal injury with a MASCIS impactor and divided into 4 groups: control, surgical control, spinal cord injury, and one cell-treated lesion group. Mononuclear cells from umbilical cord blood of human male neonates were transplanted in two experiments: a) 1 h after surgery, into the injury site at a concentration of 5 x 10(6) cells diluted in 10 µL 0.9% NaCl (N = 8-10 per group); b) into the cisterna magna, 9 days after lesion at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spinal cord injury; Cell therapy; Functional recovery; Mononuclear cells; Human umbilical cord blood; MASCIS impactor.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100009

Brain ischemia alters platelet ATP diphosphohydrolase and 5'-nucleotidase activities in naive and preconditioned rats
Provedor de dados: 56
Autores: Frassetto,S.S.; Schetinger,M.R.C.; Schierholt,R.; Webber,A.; Bonan,C.D.; Wyse,A.T.; Dias,R.D.; Netto,C.A.; Sarkis,J.J.F.
The effects of transient forebrain ischemia, reperfusion and ischemic preconditioning on rat blood platelet ATP diphosphohydrolase and 5'-nucleotidase activities were evaluated. Adult Wistar rats were submitted to 2 or 10 min of single ischemic episodes, or to 10 min of ischemia 1 day after a 2-min ischemic episode (ischemic preconditioning) by the four-vessel occlusion method. Rats submitted to single ischemic insults were reperfused for 60 min and for 1, 2, 5, 10 and 30 days after ischemia; preconditioned rats were reperfused for 60 min 1 and 2 days after the long ischemic episode. Brain ischemia (2 or 10 min) inhibited ATP and ADP hydrolysis by platelet ATP diphosphohydrolase. On the other hand, AMP hydrolysis by 5'-nucleotidase was increased after 2,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain ischemia; Ischemic preconditioning; Rat platelets; ATP diphosphohydrolase; 5'-nucleotidase.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100017

In vivo and in vitro effect of imipramine and fluoxetine on Na+,K+-ATPase activity in synaptic plasma membranes from the cerebral cortex of rats
Provedor de dados: 56
Autores: Zanatta,L.M.; Nascimento,F.C.; Barros,S.V.T.; Silva,G.R.R.S.; Zugno,A.I.; Netto,C.A.; Wyse,A.T.S.
The effects of in vivo chronic treatment and in vitro addition of imipramine, a tricyclic antidepressant, or fluoxetine, a selective serotonin reuptake inhibitor, on the cortical membrane-bound Na+,K+-ATPase activity were studied. Adult Wistar rats received daily intraperitoneal injections of 10 mg/kg of imipramine or fluoxetine for 14 days. Twelve hours after the last injection rats were decapitated and synaptic plasma membranes (SPM) from cerebral cortex were prepared to determine Na+,K+-ATPase activity. There was a significant decrease (10%) in enzyme activity after imipramine but fluoxetine treatment caused a significant increase (27%) in Na+,K+-ATPase activity compared to control (P&lt;0.05, ANOVA; N = 7 for each group). When assayed in vitro, the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Imipramine fluoxetine Na+; K+-ATPase cerebral cortex.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000005