Consulta: creatorFacets:"Neves-Borges,Anna Cristina"
Registros recuperados: 3
Data/hora: 01/06/2024 05:53:10

Expression analysis in response to drought stress in soybean: shedding light on the regulation of metabolic pathway genes
Provedor de dados: 74
Autores: Guimarães-Dias,Fábia; Neves-Borges,Anna Cristina; Viana,Antonio Americo Barbosa; Mesquita,Rosilene Oliveira; Romano,Eduardo; Grossi-de-Sá,Maria de Fátima; Nepomuceno,Alexandre Lima; Loureiro,Marcelo Ehlers; Alves-Ferreira,Márcio.
Metabolomics analysis of wild type Arabidopsis thaliana plants, under control and drought stress conditions revealed several metabolic pathways that are induced under water deficit. The metabolic response to drought stress is also associated with ABA dependent and independent pathways, allowing a better understanding of the molecular mechanisms in this model plant. Through combining an in silico approach and gene expression analysis by quantitative real-time PCR, the present work aims at identifying genes of soybean metabolic pathways potentially associated with water deficit. Digital expression patterns of Arabidopsis genes, which were selected based on the basis of literature reports, were evaluated under drought stress condition by Genevestigator. Genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Drought resistance; QPCR; Metabolic pathway; Bioinformatics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200004

Isolation and characterization of a promoter responsive to salt, osmotic and dehydration stresses in soybean
Provedor de dados: 74
Autores: Conforte,Alessandra Jordano; Guimarães-Dias,Fábia; Neves-Borges,Anna Cristina; Bencke-Malato,Marta; Felix-Whipps,Durvalina; Alves-Ferreira,Márcio.
Abstract Drought stress is the main limiting factor of soybean yield. Currently, genetic engineering has been one important tool in the development of drought-tolerant cultivars. A widely used strategy is the fusion of genes that confer tolerance under the control of the CaMV35S constitutive promoter; however, stress-responsive promoters would constitute the best alternative to the generation of drought-tolerant crops. We characterized the promoter of α-galactosidase soybean (GlymaGAL) gene that was previously identified as highly up-regulated by drought stress. The β-glucuronidase (GUS) activity of Arabidopsis transgenic plants bearing 1000- and 2000-bp fragments of the GlymaGAL promoter fused to the uidA gene was evaluated under air-dried, polyethylene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Α-galactosidase gene; Salt stress; Drought-tolerant soybean; Cis-acting element; Raffinose degradation pathway.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200226

Expression pattern of drought stress marker genes in soybean roots under two water deficit systems
Provedor de dados: 74
Autores: Neves-Borges,Anna Cristina; Guimarães-Dias,Fábia; Cruz,Fernanda; Mesquita,Rosilene Oliveira; Nepomuceno,Alexandre Lima; Romano,Eduardo; Loureiro,Marcelo Ehlers; Grossi-de-Sá,Maria de Fátima; Alves-Ferreira,Márcio.
The study of tolerance mechanisms for drought stress in soybean is fundamental to the understanding and development of tolerant varieties. Using in silico analysis, four marker genes involved in the classical ABA-dependent and ABA-independent pathways of drought response were identified in the Glycine max genome in the present work. The expression profiles of the marker genes ERD1-like, GmaxRD20A-like, GmaxRD22-like and GmaxRD29B-like were investigated by qPCR in root samples of drought sensitive and tolerant soybean cultivars (BR 16 and Embrapa 48, respectively), submitted to water deficit conditions in hydroponic and pot-based systems. Among the four putative soybean homologs to Arabidopsis genes investigated herein, only GmaxRD29B-like was not regulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water deficit; Drought adaptation; Marker genes; Glycine max.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200003