Consulta: creatorFacets:"Nicolau, Elodie"
Registros recuperados: 14
Data/hora: 02/06/2024 11:11:19

Maldi mass spectrometry imaging applied to small molecules localization
Provedor de dados: 5
Autores: Guitton, Yann; Mondeguer, Florence; Nicolau, Elodie; Royer, Florence; Hilbert, Jean-louis; Fournier, Isabelle; Wisztorski, Maxence.
MALDI Mass Spectrometry Imaging (MALDI-MSI) is a two-dimensional MALDI mass spectrometric technique used to visualize the spatial distribution of a large variety of biological molecules (from small metabolites to large proteins) without extraction, purification, separation or labelling of biological samples. Since its introduction by Caprioli et al in 1997, it has become one of the most important molecular histology methods for biomarker hunting and for understanding the spatial distribution of biomolecules in various tissues. MALDI-MSI is thus ideal for complementing the expanding field of metabolomics. Despite the fact that MALDI-MSI have been very successfully applied in divers studies (even in clinical applications) its use remains far from routine,...
Tipo: Text
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00179/29041/27479.pdf

Use comparative proteomics on a selected microalgae provides candidates for biofuel production.
Provedor de dados: 5
Autores: Garnier, Matthieu; Carrier, Gregory; Rogniaux, Helene; Nicolau, Elodie; Bougaran, Gael; Saint-jean, Bruno; Cadoret, Jean-paul.
Microalgae are photosynthetic microorganisms that have an extraordinary diversity which biotechnological potential is just beginning to be explored. Microalgae in biotechnology are presently the focus of an unprecedented surge in interest and investment worldwide. Understanding microalgal lipid accumulation under nitrogen starvation is of major interest for feedstocks, food and biofuel production. Although studies have been performed in model species, to this day it is critical to understand through particular mechanisms of biotechnological species. Tisochrysis lutea is traditionally used for aquaculture feed and presents advantages for lipid production. The wild type strain and a lipid over-accumulating selected mutant were used to perform the first...
Tipo: Text
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00176/28735/27203.pdf

Community analysis of pigment patterns from 37 microalgae strains reveals new carotenoids and porphyrins characteristic of distinct strains and taxonomic groups
Provedor de dados: 5
Autores: Serive, Benoit; Nicolau, Elodie; Berard, Jean-baptiste; Kaas, Raymond; Pasquet, Virginie; Picot, Laurent; Cadoret, Jean-paul.
Phytoplankton, with an estimated 30 000 to 1 000 000 species clustered in 12 phyla, presents a high taxonomic and ecophysiological diversity, reflected by the complex distribution of pigments among the different algal classes. High performance liquid chromatography is the gold standard method for qualitative and quantitative analysis of phytoplankton pigments in seawater and culture samples, but only a few pigments can be used as robust chemotaxonomic markers. A major challenge is thus to identify new ones, characteristic of a strain, species, class or taxon that cannot be currently identified on the basis of its pigment signature. Using an optimized extraction process coupled to a HPLC de-replication strategy, we examined the pigment composition of 37...
Tipo: Text
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00373/48416/48627.pdf

Comparative proteomics reveals proteins impacted by nitrogen deprivation in wild-type and high lipid-accumulating mutant strains of Tisochrysis lutea
Provedor de dados: 5
Autores: Garnier, Matthieu; Carrier, Gregory; Rogniaux, Helene; Nicolau, Elodie; Bougaran, Gael; Saint-jean, Bruno; Cadoret, Jean-paul.
Understanding microalgal lipid accumulation under nitrogen starvation is of major interest for biomass feedstock, food and biofuel production. Using a domesticated oleaginous algae Tisochrysis lutea, we performed the first comparative proteomic analysis on the wild type strain and a selected lipid over-accumulating mutant. 2-DE analysis was made on these strains cultured in two metabolic conditions, with and without nitrogen deprivation, which revealed significant differences in proteomes according to both strain and nitrogen availability. Mass spectrometry allowed us to identify 37 proteins that were differentially expressed between the two strains, and 17 proteins regulated by nitrogen starvation concomitantly with lipid accumulation. The proteins...
Tipo: Text
Palavras-chave: Microalgae; Biotechnology; Lipid; Nitrogen; Proteomic; Selection.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00179/29012/27470.pdf

A Bibliometric Analysis of Microalgae Research in the World, Europe, and the European Atlantic Area
Provedor de dados: 5
Autores: Rumin, Judith; Nicolau, Elodie; Gonçalves De Oliveira Junior, Raimundo; Fuentes-grünewald, Claudio; Flynn, Kevin J; Picot, Laurent.
A bibliographic database of scientific papers published by authors affiliated worldwide, especially focused in Europe and in the European Atlantic Area, and containing the keywords “microalga(e)” or “phytoplankton” was built. A corpus of 79,020 publications was obtained and analyzed using the Orbit Intellixir software to highlight the evolution of the research domain. Publication rates from 1960 to 2019, organization of the research, collaboration networks between countries and organizations, emerging and fading research concepts, major studied species, and associated concepts, as well as journals publishing microalgae research were considered. As a result, of the 79,020 papers published worldwide, 26,137 included authors from Europe (33% of world...
Tipo: Text
Palavras-chave: Biotechnology; European Atlantic Area; Microalgae; Phytoplankton; Research; Market; Bibliometrics.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00605/71725/70191.pdf

Carotenoids from Rhodomonas salina Induce Apoptosis and Sensitize A2058 Melanoma Cells to Chemotherapy
Provedor de dados: 5
Autores: De Oliveira-júnior, Raimundo Gonçalves; Nicolau, Elodie; Bonnet, Antoine; Prunier, Grégoire; Beaugeard, Laureen; Joguet, Nicolas; Thiéry, Valérie; Picot, Laurent.
Melanoma is an aggressive tumor with invasive and metastatic potential, frequently exhibiting multidrug resistance mechanisms. In our continuous search for antimelanoma molecules, we have identified some effective marine compounds capable of not only inducing cell death, but also of sensitizing chemoresistant tumor cells to clinically used anticancer drugs. In this report, the cryptophyte Rhodomonas salina (Wislouch) D.R.A.Hill &amp; R.Wetherbee, Pyrenomonadaceae, was chemically investigated in order to identify pigments efficiently inhibiting melanoma cells proliferation. All pharmacological tests were performed on A2058 cells expressing the oncogenic BRAF V600E mutation and resistant to dacarbazine treatment. Flash chromatography of R. salina ethanol...
Tipo: Text
Palavras-chave: Alloxanthin; Chemosensitivity; Crocoxanthin; Cutaneous melanoma; Drug resistance; Microalgae.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00630/74194/73918.pdf

Cobalamin Scarcity Modifies Carbon Allocation and Impairs DMSP Production Through Methionine Metabolism in the Haptophyte Microalgae Tisochrysis lutea
Provedor de dados: 5
Autores: Nef, Charlotte; Henry, Céline; Nicolau, Elodie; Bérard, Jean-baptiste; Hervé, Fabienne; Caruana, Amandine; Kaas, Raymond; Mairet, Francis; Garnier, Matthieu.
Cobalamin (vitamin B12) is a cobalt-containing enzymatic cofactor involved in methionine synthesis. Provided only by select bacteria and archaea in marine systems, this vitamin is known to limit primary production in different oceanic areas. Understanding the consequences of cobalamin limitation on phytoplankton physiology is of great interest, notably for cobalamin-dependent haptophytes that significantly contribute to oceanic carbon fixation and sulfur cycle through dimethyl sulfonio propionate (DMSP) synthesis. Here, the effect of cobalamin limitation was compared to nitrogen limitation on the model haptophyte Tisochrysis lutea grown in chemostats, combining comparative proteomics with the analysis of major macromolecules and specific osmolytes. Our...
Tipo: Text
Palavras-chave: Cobalamin; Vitamin B-12; Haptophyte microalgae; Tisochrysis lutea; DMSP; Chemostat; Comparative proteomics; Metabolism.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00654/76575/77711.pdf

Recent developments in the detection of phycotoxins
Provedor de dados: 5
Autores: Hess, Philipp; Nicolau, Elodie.
Over the past seven years, methods available for the detection of phycotoxins have been extensively reviewed in a number of international expert committees, such as the consultations organised by FAO/IOC/WHO and EFSA, as well as by individual scientists. These reviews have shown that the methods available have severe limitations for the use in official control, either due to their limited scope and detection capability or due to a lack of calibration standards, reference materials and validation efforts. The present review focuses on recent developments in the detection of phycotoxins in several areas of applied research. Not being able to exhaustively describe all recent developments, the review focussed on three areas of interest to the authors: (i)...
Tipo: Text
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00019/12975/9949.pdf

Azaspiracid accumulation, detoxification and biotransformation in blue mussels (Mytilus edulis) experimentally fed Azadinium spinosum
Provedor de dados: 5
Autores: Jauffrais, Thierry; Marcaillou-le Baut, Claire; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Nicolau, Elodie; Tillmann, Urban; Hess, Philipp.
Azadinium spinosum (Elbrächter and Tillmann), a small marine dinoflagellate, has been recently described as a de novo producer of azaspiracid-1 and -2 (AZA1 and -2) diarrhoeic toxins. A culture of A. spinosum was established in our laboratory and optimised for pilot-scale production of this organism, to evaluate and understand AZA1 and -2 accumulation and biotransformation in blue mussels (Mytilus edulis) fed with A. spinosum.  Adult mussels were continuously exposed to A. spinosum over 1 week in 160 L cylindrical conical tanks. Three different diets were tested for contamination: 5000, 10 000 cells mL−1 of A. spinosum and a mixture of 5000 cells mL−1 of A. spinosum with 5000 cells mL−1 of Isochrysis aff. galbana (T-Iso, CCAP 927/14). During the subsequent...
Tipo: Text
Palavras-chave: Azaspiracid; Azadinium spinosum; Marine biotoxins; AZA; Tissue distribution; Histology; Bivalve molluscs; Liquid chromatography coupled to tandem mass spectrometry.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00088/19877/17566.pdf

Impact of Light Intensity on Antioxidant Activity of Tropical Microalgae
Provedor de dados: 5
Autores: Coulombier, Noémie; Nicolau, Elodie; Le Déan, Loic; Antheaume, Cyril; Jauffrais, Thierry; Lebouvier, Nicolas.
Twelve microalgae species isolated in tropical lagoons of New Caledonia were screened as a new source of antioxidants. Microalgae were cultivated at two light intensities to investigate their influence on antioxidant capacity. To assess antioxidant property of microalgae extracts, four assays with different modes of action were used: 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azinobis (3-éthylbenzothiazoline-6-sulphonique) (ABTS), oxygen radical absorbance capacity (ORAC), and thiobabituric acid reactive substances (TBARS). This screening was coupled to pigment analysis to link antioxidant activity and carotenoid content. The results showed that none of the microalgae studied can scavenge DPPH and ABTS radicals, but Chaetoceros sp., Nephroselmis sp., and...
Tipo: Text
Palavras-chave: Nephroselmis; Light intensity; In vitro antioxidant activity; Siphonaxanthin; Carotenoid; Bioactive compounds.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00609/72152/70900.pdf

Analysis of Scientific Research Driving Microalgae Market Opportunities in Europe
Provedor de dados: 5
Autores: Rumin, Judith; Nicolau, Elodie; Junior, Raimundo Gonçalves De Oliveira; Fuentes-grünewald, Claudio; Picot, Laurent.
A bibliographic database of scientific papers published by authors affiliated to research institutions worldwide, especially focused in Europe and in the European Atlantic Area, and containing the keywords “microalga(e)” or “phytoplankton” was built. A corpus of 79,020 publications was obtained and analyzed using the Orbit Intellixir software to characterize the research trends related to microalgae markets, markets opportunities and technologies that could have important impacts on markets evolution. Six major markets opportunities, the production of biofuels, bioplastics, biofertilizers, nutraceuticals, pharmaceuticals and cosmetics, and two fast-evolving technological domains driving markets evolution, microalgae harvesting and extraction technologies...
Tipo: Text
Palavras-chave: Bibliometric; Microalgae; Biofertilizers; Bioplastics; Biotechnology; Cosmetics; Europe; Food; GMO; Market; Nutraceuticals; Pharmaceuticals; Research.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00630/74185/73788.pdf

UPLC-MSE Profiling of Phytoplankton Metabolites: Application to the Identification of Pigments and Structural Analysis of Metabolites in Porphyridium purpureum
Provedor de dados: 5
Autores: Juin, Camille; Bonnet, Antoine; Nicolau, Elodie; Berard, Jean-baptiste; Devillers, Romain; Thiery, Valerie; Cadoret, Jean-paul; Picot, Laurent.
A fast and high-resolution UPLC-MSE analysis was used to identify phytoplankton pigments in an ethanol extract of Porphyridium purpureum (Pp) devoid of phycobiliproteins. In a first step, 22 standard pigments were analyzed by UPLC-MSE to build a database including retention time and accurate masses of parent and fragment ions. Using this database, seven pigments or derivatives previously reported in Pp were unequivocally identified: beta,beta-carotene, chlorophyll a, zeaxanthin, chlorophyllide a, pheophorbide a, pheophytin a, and cryptoxanthin. Minor amounts of Divinyl chlorophyll a, a chemotaxonomic pigment marker for prochlorophytes, were also unequivocally identified using the database. Additional analysis of ionization and fragmentation patterns...
Tipo: Text
Palavras-chave: Carotenoid; Chlorophyll; Dereplication; Divinyl chlorophyll a; Galactosyldiacylglycerol; Gracilamide; Mass spectrometry; MSE; Phytoplankton; Pigment; Porphyridium purpureum; UPLC.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00269/37994/36069.pdf

Effects of Nitrogen Availability on the Antioxidant Activity and Carotenoid Content of the Microalgae Nephroselmis sp
Provedor de dados: 5
Autores: Coulombier, Noémie; Nicolau, Elodie; Le Déan, Loic; Barthelemy, Vanille; Schreiber, Nathalie; Brun, Pierre; Lebouvier, Nicolas; Jauffrais, Thierry.
Nephroselmis sp. was previously identified as a species of interest for its antioxidant properties owing to its high carotenoid content. In addition, nitrogen availability can impact biomass and specific metabolites’ production of microalgae. To optimize parameters of antioxidant production, Nephroselmis sp. was cultivated in batch and continuous culture conditions in stirred closed photobioreactors under different nitrogen conditions (N-repletion, N-limitation, and N-starvation). The aim was to determine the influence of nitrogen availability on the peroxyl radical scavenging activity (oxygen radical absorbance capacity (ORAC) assay) and carotenoid content of Nephroselmis sp. Pigment analysis revealed a specific and unusual photosynthetic system with...
Tipo: Text
Palavras-chave: Lutein; Natural products; Nutrients; Peroxyl radical; Siphonaxanthin.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00645/75699/76604.pdf

Zeaxanthin from Porphyridium purpureum induces apoptosis in human melanoma cells expressing the oncogenic BRAF V600E mutation and sensitizes them to the BRAF inhibitor vemurafenib
Provedor de dados: 5
Autores: Juin, Camille; De Oliveira Junior, Raimundo Gonsalves; Fleury, Audrey; Oudinet, Chloe; Pytowski, Lior; Berard, Jean-baptiste; Nicolau, Elodie; Thiery, Valerie; Lanneluc, Isabelle; Beaugeard, Laureen; Prunier, Gregoire; Guedes Da Silva Almeida, Jackson Roberto; Picot, Laurent.
Zeaxanthin, an abundant carotenoid present in fruits, vegetables and algae was reported to exert antiproliferative activity and induce apoptosis in human uveal melanoma cells. It also inhibited uveal melanoma tumor growth and cell migration in nude mice xenograft models. Here we report that zeaxanthin purified from the rhodophyte Porphyridium purpureum (Bory) K.M.Drew &amp; R.Ross, Porphyridiaceae, promotes apoptosis in the A2058 human melanoma cell line expressing the oncogenic BRAF V600E mutation. Zeaxanthin 40 μM (IC50) induced chromatin condensation, nuclear blebbing, hypodiploidy, accumulation of cells in sub-G1 phase, DNA internucleosomal fragmentation and activation of caspase-3. Western blot analysis revealed that zeaxanthin induced up-regulation...
Tipo: Text
Palavras-chave: Cancer; Carotenoid; Melanoma; Microalgae; Phytoplankton; Zeaxanthin.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00444/55595/57227.pdf