Consulta: creatorFacets:"Nirchio,Mauro"
Registros recuperados: 10
Data/hora: 06/06/2024 13:51:32

Extensive polymorphism and chromosomal characteristics of ribosomal DNA in the characid fish Triportheus venezuelensis (Characiformes, Characidae)
Provedor de dados: 74
Autores: Nirchio,Mauro; Oliveira,Claudio; Ferreira,Irani Alves; Granado,Angel; Ron,Ernesto.
The karyotype and chromosomal characteristics of the characid fish Triportheus venezuelensis were investigated using differential staining techniques (C-banding, Ag-NOR staining) and fluorescent in situ hybridization (FISH) with an 18S rDNA probe. The diploid chromosome number (2n = 52), karyotype composition and sex chromosome determination system of the ZZ/ZW type were the same as previously described in other species of the genus Triportheus. However, extensive variation regarding nucleolus organizer regions (NOR) different from other species was observed. 18S rDNA sequences were distributed on nine chromosome pairs, but the number of chromosomes with Ag-NORs was usually lower, reaching a maximum of four chromosomes. When sequential staining experiments...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 18S rDNA; Ag-NORs; C-band; FISH; Fish cytogenetics; Sex chromosomes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100007

Cytogenetic analysis of three sea catfish species (Teleostei, Siluriformes, Ariidae) with the first report of Ag-NOR in this fish family
Provedor de dados: 74
Autores: Nirchio,Mauro; Martinez,Emanuel Ricardo Monteiro; Foresti,Fausto; Oliveira,Claudio.
Despite their ecological and economical importance, fishes of the family Ariidae are still genetically and cytogenetically poorly studied. Among the 133 known species of ariids, only eight have been karyotyped. Cytogenetic analyses performed on Genidens barbus and Sciades herzbergii revealed that both species have 2n = 56 chromosomes and Cathorops aff. mapale has 2n = 52 chromosomes: Genidens barbus has 10 Metacentrics (M), 14 Submetacentrics (SM), 26 Subtelocentrics (ST), and 6 Acrocentrics (A), Sciades herzbergii has 14M, 20SM, 18ST and 4A, whereas Cathorops aff. mapale has 14M, 20SM, and 18ST. The nucleolus organizer regions (NORs) were found in a single chromosome pair on the short arm of a large-sized ST pair in Genidens barbus and on the short arm of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; Ag-NORs; Chromosome evolution; Chromosome rearrangements; Fish.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200011

Cytogenetic analysis of three catfish species of the family Pseudopimelodidae (Teleostei, Siluriformes)
Provedor de dados: 74
Autores: Martinez,Emanuel Ricardo Monteiro; Nirchio,Mauro; Granado,Angel; Foresti,Fausto; Oliveira,Claudio.
Cytogenetic analyses performed in Cephalosilurus apurensis, Microglanis aff. cottoides and Pseudopimelodus bufonius revealed that the three species have 2n = 54 chromosomes: C. apurensis presented six metacentric (M), 28 submetacentric (SM), 14 subtelolocentric (ST), and six acrocentric (A) chromosomes, while M. aff. cottoides showed 10M, 32SM, 10ST and 2A, and P. bufonius had 12M, 30SM and 12ST. The nucleolus organizer regions (NORs) were present on the short arm of a middle-sized ST pair, identified as pair 19, in C. apurensis NORs were found on the short arm of a middle-sized ST (pair 23) and on the long arm of a middle-sized ST (pair 22) in M. aff. cotttoides and on the short arm of three middle-sized ST pairs, identified as pairs 9, 10 and 11, in P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Chromosomes; C-banding; Ag-NOR; Fish.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400015

Cytogenetic studies in three species of Lutjanus (Perciformes: Lutjanidae: Lutjaninae) from the Isla Margarita, Venezuela
Provedor de dados: 83
Autores: Nirchio,Mauro; Rondón,Rodolfo; Oliveira,Claudio; Ferreira,Irani A.; Martins,Cesar; Pérez,Julio; Sola,Luciana; Rossi,Anna Rita.
In the present study, three species of Lutjaninae, Lutjanus analis, L. griseus and L. synagris, were analyzed by conventional Giemsa staining, C-banding and silver staining, to reveal active Nucleolus Organizer Regions (NORs). Fluorescent in situ hybridization (FISH) was also applied to establish the number and location of the ribosomal gene clusters (18S and 5S rRNA genes). Counts of diploid metaphasic cells revealed a diploid modal chromosome complement composed of 48 acrocentric chromosomes in both L. analis and L. griseus. Two cytotypes were observed in L. synagris: cytotype I, with 2n=48 acrocentric chromosomes, found in 19 specimens, and cytotype II, with 46 acrocentric chromosomes and one large metacentric, found in two specimens. The large...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Ribosomal genes; NOR polymorphism; C-banding; Robertsonian rearrangement.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000100012

Chromosome evolution in fishes: a new challenging proposal from Neotropical species
Provedor de dados: 83
Autores: Nirchio,Mauro; Rossi,Anna Rita; Foresti,Fausto; Oliveira,Claudio.
We present a database containing cytogenetic data of Neotropical actinopterygian fishes from Venezuela obtained in a single laboratory for the first time. The results of this study include 103 species belonging to 74 genera assigned to 45 families and 17 out of the 40 teleost orders. In the group of marine fishes, the modal diploid number was 2n=48 represented in 60% of the studied species, while in the freshwater fish group the modal diploid complement was 2n=54, represented in 21.21 % of the studied species. The average number of chromosomes and the mean FN were statistically higher in freshwater fish than in marine fish. The degree of diversification and karyotype variation was also higher in freshwater fish in contrast to a more conserved cytogenetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actinopterygii; Ancestral karyotype; Diploid number; Fundamental number.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400761

Cytogenetic characterization of Rhomboplites aurorubens and Ocyurus chrysurus, two monotypic genera of Lutjaninae from Cubagua Island, Venezuela, with a review of the cytogenetics of Lutjanidae (Teleostei: Perciformes)
Provedor de dados: 83
Autores: Nirchio,Mauro; Oliveira,Claudio; Ferreira,Daniela C.; Rondón,Rodolfo; Pérez,Julio E.; Hett,Anne Kathrin; Rossi,Anna Rita; Sola,Luciana.
Lutjanidae, commonly known as snappers, includes 105 species, grouped in four subfamilies. In spite of the high number of species and of its worldwide distribution, the family has been little investigated and the phylogenetic relationships among some of its genera and species are still cause for debate. Only a small number of the species has been cytogenetically analysed. This study reports the first description of the karyotype of Rhomboplites aurorubens as well as data concerning the distribution of the constitutive heterochromatin and the location of the 18S rRNA and the 5S rRNA genes. Specimens of Ocyurus chrysurus from Venezuela were also investigated for the same cytogenetic features. Both species have a 48 uniarmed karyotype, but R. aurorubens has a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-banding FISH; Karyotype NORs Ribosomal genes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400005

Chromosome analysis in Saccodon wagneri (Characiformes) and insights into the karyotype evolution of Parodontidae
Provedor de dados: 83
Autores: Nirchio,Mauro; Masache,Maria Cecilia; Paim,Fabilene Gomes; Cioffi,Marcelo de Bello; Moreira Filho,Orlando; Barriga,Ramiro; Oliveira,Claudio; Rossi,Anna Rita.
ABSTRACT Parodontidae is a relatively small group of Neotropical characiform fishes consisting of three genera (Apareiodon, Parodon, and Saccodon) with 32 valid species. A vast cytogenetic literature is available on Apareiodon and Parodon, but to date, there is no cytogenetic data about Saccodon, a genus that contains only three species with a trans-Andean distribution. In the present study the karyotype of S. wagneri was described, based on both conventional (Giemsa staining, Ag-NOR, C-bands) and molecular (repetitive DNA mapping by fluorescent in situ hybridization) methods. A diploid chromosome number of 2n = 54 was observed in both sexes, and the presence of heteromorphic sex chromosomes of the ZZ/ZW type was detected. The W chromosome has a terminal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ag-NOR; Cytogenetics; FISH; Heterochromatin; ZW sex chromosomes.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100203

Karyotypic characterization of Prochilodus mariae, Semaprochilodus kneri and S. laticeps(Teleostei: Prochilodontidae) from Caicara del Orinoco, Venezuela
Provedor de dados: 83
Autores: Oliveira,Claudio; Nirchio,Mauro; Granado,Ángel; Levy,Sara.
Fish of the family Prochilodontidae are considered one of the most important components of commercial and subsistence fishery in freshwater environments in South America. This family consists of 21 species and three genera. In the present study, the karyotypes of Prochilodus mariae, Semaprochilodus kneri, and S. laticeps from Caicara del Orinoco, Bolivar State, Venezuela were studied. The species P. mariae, S. kneri and S. laticeps exhibited 2n=54 chromosomes (40 metacentric and 14 submetacentric), a single chromosome pair with nucleolus organizer regions, and a large amount of heterochromatin found at centromeric and pericentromeric positions in almost all chromosomes. The P. mariae specimens studied displayed 0 to 3 supernumerary microchromosomes. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; C-band; NOR banding; Chromossome evolution; Characiformes.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252003000100005

Karyotype characterization of Mugil incilis Hancock, 1830 (Mugiliformes: Mugilidae), including a description of an unusual co-localization of major and minor ribosomal genes in the family
Provedor de dados: 83
Autores: Hett,Anne Kathrin; Nirchio,Mauro; Oliveira,Claudio; Siccha,Zoila Raquel; Rossi,Anna Rita; Sola,Luciana.
This study reports the description of the karyotype of Mugil incilis from Venezuela. The chromosome complement is composed of 48 acrocentric chromosomes, which uniformly decrease in size. Therefore, the homologues can not be clearly identified, with the exception of one of the largest chromosome pairs, classified as number 1, whose homologues may show a subcentromeric secondary constriction, and of chromosome pair number 24, which is considerably smaller than the others. C-banding showed heterochromatic blocks at the centromeric/pericentromeric regions of all chromosomes, which were more conspicuous on chromosomes 1, given the C-positive signals include the secondary constrictions. AgNO3 and fluorescent in situ hybridization (FISH) with 45S rDNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-banding; COI; 5S rDNA; Mullet; NORs.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000100008

Cytogenetical and morphological features reveal significant differences among Venezuelan and Brazilian samples of Mugil curema (Teleostei: Mugilidae)
Provedor de dados: 83
Autores: Nirchio,Mauro; Cipriano,Roger; Cestari,Margarete; Fenocchio,Alberto.
Karyotype of M. curema from the Gulf of Mexico and Brazil have been reported as possessing chromosome complement with 2n=28 and FN=48, whereas specimens from Venezuela has been reported as possessing a diploid number 2n=24 and a conserved FN (48). Although at first sight this variation suggests the presence of a chromosomal intraspecific (interpopulational) variability, the possibility that we are dealing with two different species was examined. This work revisit the karyotypes of M. curema from Venezuela and Brazil, including new data on C-banding, and NOR localization, and compares morphologic characteristics of samples from both localities. Thus, besides diploid number, the constitutive heterochromatin distribution and NORs location, mark other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; C-band; NOR-banding; Meristic and morphometric features; Species identification.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252005000100006