Consulta: creatorFacets:"Noiret, Christophe"
Registros recuperados: 3
Data/hora: 11/06/2024 03:35:25

Obtention de souches conchylicoles performantes par polyploïdisation (3ème partie)
Provedor de dados: 5
Autores: Gerard, Andre; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Noiret, Christophe; Bodoy, Alain; Heurtebise, Serge; Garnier, Jacqueline.
The third part of the polyploidisation program of the main French species of commercial interest, this report presents the actions and results for the year 1991 which was devoted to:- continuation of the development trials of the triploidy induction technique in Ruditapes decussates (i.e. indigenous clam)- production of diploid and triploid populations of Ostrea edulis (i.e. flat oyster)- control of diploid and triploid population performances in cupped oyster and Pacific clam in their natural habitat- continuation of the development of a ploidy control technique through image analysis- beginning of the development of a new polyploidy induction technique in bivalve molluscs
Tipo: Text
Palavras-chave: Ruditapes decussatus; Ostrea edulis; Ruditapes philippinarum; Crassostrea gigas; Imagerie numérique; Cytogénétique; Polyploïdie.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/rapport-3857.PDF

Induced triploidy in the European clam, Ruditapes decussatus (L.) and performance of triploid larvae
Provedor de dados: 5
Autores: Gerard, Andre; Naciri, Yamama; Noiret, Christophe; Ledu, Christophe; Peignon, Jean-marie.
For the first time, effective treatments using cytochalasin B were developed to induce triploidy in the European clam, Ruditapes decussatus (L.). The percentage of triploid embrios was assessed by karyological or image analysis. Two treatments (O.5 or 1 mg of cytochasin B (CB per ml of dimethylsulfoxide in 1 l of sea water) were applied at different times after fertilization (10 to 25 mn), for two different periods (15 and 20 min). Best results were obtained for a CB concentration of 1 mg/l. When treatment was applied 15 min after fertilization for a 20 min period, 94% and 95% of triploid embryos were obtained in two repeated experiments. At metamorphosis, the treated larvae appeared to be no smaller than the control larvae in all experiments. Thowever, in...
Tipo: Text
Palavras-chave: Genetic; Ruditapes decussatus; Clam; Induced triploidy.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/1994/publication-3208.pdf

Image analysis : a new method for estimating triploidy in commercial bivalves
Provedor de dados: 5
Autores: Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Noiret, Christophe; Peudenier, Isabelle; Grizel, Henri.
For determination of ploidy levels in bivalves,  three techniques are now routinely used: karyological determination. microfluorimetry and flow cytometry. This paper proposes an alternative technique which is based on determination of the optical density (OD) of stained nuclei using image analysis. A karyological and image analysis comparative experiment conducted on juveniles of Crassostrea gigas (Thunberg) and Ruditapes philippinarum (Adam &amp; Reeve) is described and highlights the advantages of the image analysis technique. Initially developed on C. gigas and R. philippinarum, the image analysis method was easily extended to other bivalve species and to different developmental stages, as demonstrated by two experiments conducted on Ostrea edulis (L.)....
Tipo: Text
Palavras-chave: Estimation bivalve triploidy; Image analysis; Triploid; Genetic; Crassostrea gigas; Oysters.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/1994/publication-3200.pdf