Consulta: creatorFacets:"Norais, Cédric"
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A novel proteomic approach identifies new interaction partners for proliferating cell nuclear antigen
Provedor de dados: 5
Autores: Mesiet Cladiere, L; Norais, Cédric; Kuhn, Joelle; Briffotaux, Julien; Sloostra, Jerry W.; Ferrari, Elena; Hubscher, Ulrich; Flament, Didier; Myllykallio, Hannu.
During DNA replication and repair, many proteins bind to and dissociate in a highly specific and ordered manner from proliferating cell nuclear antigen (PCNA). We describe a combined approach of in silico searches at the genome level and combinatorial peptide synthesis to investigate the binding properties of hundreds of short PCNA-interacting peptides (PIP-peptides) to archaeal and eukaryal PCNAs. Biological relevance of our combined approach was demonstrated by identification an inactive complex of Pyrococcus abyssi ribonuclease HII with PCNA. Furthermore we show that PIP-peptides interact with PCNA largely in a sequence independent manner. Our experimental approach also identified many so far unidentified PCNA interacting peptides in a number of human...
Tipo: Text
Palavras-chave: Ribonuclease HII; Combinatorial peptide synthesis; PCNA.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3297.pdf