Consulta: creatorFacets:"Ohresser, M"
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Data/hora: 11/06/2024 06:55:14

Variable microsatellites in the Pacific Oyster Crassostrea gigas and other cupped oyster species
Provedor de dados: 5
Autores: Huvet, Arnaud; Boudry, Pierre; Ohresser, M; Delsert, Claude; Bonhomme, F.
Source/description : Genomic DNA was extracted from a whole Pacific oyster (C. gigas) after grinding in liquid nitrogen. Purified DNA was then digested by a mix of 3 restriction enzymes (AluI, RsaI, HaeIII). DNA fragments ranging from 250 to 500 bp were size-selected by agarose gel electrophoresis and ligated into the dephosphorylated blunt-ended SmaI-linearised pBKS2 plasmid (Stratagene Cloning System, CA). White colonies (11,500) were screened by hybridisation with double-stranded DNA probes containing dinucleotide repeats. Poly(dAdC), poly(dAdG), poly(dAdT) (Pharmacia Biotech, Sweden) were mixed at equimolar ratio and radiolabelled by [&amp;#61537;-32P] alpha dATP and dCTP using a random priming labelling kit (Life Technologies, Germany). Filter...
Tipo: Text
Palavras-chave: Crassostrea gigas; Oyster; Microsatellites.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-703.pdf