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Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571
Provedor de dados: 3
Autores: Oliveira, Luana Paula de Azevedo de; Oliveira, Tiago Felipe; Contiero, Jonas; Cazetta, Márcia Luciana.
The factorial planning was used to plan and optimize inulinase production by the yeast Kluyveromyces marxianus NRRL Y-7571. The experiments were conducted using a Central Composite Design (CCD) 22, at different concentrations of agave syrup (3.6 to 6.4%) and yeast extract (2.2 to 3.0%). After 96 hours of fermentation, the best condition for the inulinase production was 5% agave syrup and 2.5% yeast extract, which yielded an average of 129.21 U mL-1 of inulinase. Partial characterization of the crude enzyme showed that the optimal pH and temperature were 4.0 and 60°C, respectively. The enzyme showed thermal stability at 55°C for 4 hours. 
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.12.02.00-1 yeast; Microbial enzymes; Agricultural substrate Microbiologia aplicada.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31489