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Detection of hepatitis C virus RNA in saliva samples from patients with seric anti-HCV antibodies
Provedor de dados: 55
Autores: Gonçalves,Patrícia L.; Cunha,Carla B.; Busek,Solange C. U.; Oliveira,Guilherme C.; Ribeiro-Rodrigues,Rodrigo; Pereira,Fausto EL.
We examined the frequency of HCV-RNA in saliva samples from anti-HCV positive patients. Both plasma and saliva samples from 39 HCV patients (13 with normal liver enzymes, 19 with abnormal liver enzymes and 13 with cirrhosis) were investigated. Stimulated saliva and fresh plasma were centrifuged (900 x g,10 min) and stored at -70ºC, after the addition of guanidine isothiocyanate RNA extraction buffer. HCV-RNA was detected by RT- nested-PCR (amplification of HCV-cDNA for two rounds, using HCV primers 939/209 and 940/211). HCV genotyping was carried out by RFLP (using Mva I and Hinf 1 or Hae III and Rsa I restriction enzymes). Thirty-two out of 39 (82%; 95% CI=70-94%) anti-HCV-positive patients had HCV-RNA in plasma samples. Eight out of 39 (20.5%; 95%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HCV-RNA in saliva; Hepatitis C; Saliva.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100006