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Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro
Provedor de dados: 48
Autores: Pan,Zeya; Pan,Hao; Zhang,Jin; Yang,Yun; Liu,Hui; Yang,Yuan; Huang,Gang; Ni,Junsheng; Huang,Jian; Zhou,Weiping.
BACKGROUND: Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells. RESULTS: In the present study, we investigated the functions of USP39 in human hepatocellular carcinoma (HCC) cell line SMMC-7721. We knocked down the expression of USP39 through lentivirus mediated RNA interference. The results of qRT-PCR and western blotting assay showed that both the mRNA and protein levels were suppressed efficiently after USP39 specific shRNA was delivered into SMMC-7721 cells. Cell growth was significantly inhibited as determined by MTT assay. Crystal violet staining indicated that colony numbers and sizes were both reduced after...
Tipo: Journal article
Palavras-chave: Ubiquitin Specific Peptidase 39; Lentivirus; Human hepatocellular carcinoma; Cell proliferation; Cell cycle.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100018