Consulta: creatorFacets:"Panserat, Stéphane"
Registros recuperados: 5
Data/hora: 17/06/2024 13:48:21

Hepatic phosphoenolpyruvate carboxykinase gene expression is not repressed by dietary carbohydrates in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: 5
Autores: Panserat, Stéphane; Plagnes Juan, E; Breque, Jeanine; Kaushik, Sadasivam.
Phosphoenolpyruvate carboxykinase (PEPCK) is a rate-limiting enzyme in hepatic gluconeogenesis and therefore plays a central role in glucose homeostasis. The aim of this study was to analyse the nutritional regulation of PEPCK gene expression in rainbow trout (Oncorhynchus mykiss), which are known to use dietary carbohydrates poorly. A full-length hepatic PEPCK cDNA (2637 base pairs with one open reading frame putatively encoding a 635-residue protein) was cloned and found to be highly homologous to mammalian PEPCKs. The presence of a putative peptide signal specific to a mitochondrial-type PEPCK in the deduced amino acid sequence suggests that this PEPCK gene codes for a mitochondrial form, In gluconeogenic tissues such as liver, kidney and intestine,...
Tipo: Text
Palavras-chave: Gluconeogenesis; Nutrition; Metabolism; Glucose; Oncorhynchus mykiss; Rainbow trout; Phosphoenolpyruvate carboxykinase.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-414.pdf

Lack of significant long-term effect of dietary carbohydrates on hepatic glucose-6-phosphatase expression in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: 5
Autores: Panserat, Stéphane; Medale, Francoise; Breque, Jeanine; Plagnes Juan, E; Kaushik, Sadasivam.
Hepatic glucose-6-phosphatase (G6Pase) plays an important role in glucose metabolism because it catalyzes the release of glucose to the circulatory system in the processes of glycogenolysis and gluconeogenesis. The present study was initiated to analyze the regulation of hepatic G6Pase expression by dietary carbohydrates in rainbow trout. The first step in our study was the identification of a partial G6Pase cDNA in rainbow trout that was highly homologous to that of mammals. Hepatic G6Pase activities and mRNA levels were measured in trout fed one of the experimental diets, with or without carbohydrates. We found no significant effect of intake of dietary carbohydrates on G6Pase Expression (mRNA and activity) 6 hours and 24 hours after feeding. These...
Tipo: Text
Palavras-chave: Dietary carbohydrates; Rainbow trout; Fish nutrition; Glucose 6 phosphatase expression.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-696.pdf

Gluconeogenic enzyme gene expression is decreased by dietary carbohydrates in common carp (Cyprinus carpio) and gilthead seabream (Sparus aurata)
Provedor de dados: 5
Autores: Panserat, Stéphane; Plagnes Juan, E; Kaushik, Sadasivam.
Our objective is to understand the low metabolic utilization of dietary carbohydrates in fish. We compared the regulation of gluconeogenic enzymes at a molecular level in two fish species, the common carp (Cyprinus carpio) and gilthead seabream (Sparus aurata), known to be relatively tolerant to dietary carbohydrates. After cloning of partial cDNA sequences for three key gluconeogenic enzymes (glucose-6-phosphatase (G6Pase), fructose biphosphatase (FBPase) and phosphoenolpyruvate carboxykinase (PEPCK) in the two species, we analyzed gene expressions of these enzymes 6 and 24 h after feeding with (20%) or without carbohydrates. Our data show that there is at least one gluconeogenic enzyme strongly regulated (decreased expression after feeding) in the two...
Tipo: Text
Palavras-chave: Gilthead seabream; Common carp; Gluconeogenesis; Gene expression; Hepatic glucose metabolism; Dietary carbohydrate.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-668.pdf

Molecular cloning, tissue distribution and sequence analysis of complete glucokinase cDNAs from gilthead seabream (Sparus aurata), rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio)
Provedor de dados: 5
Autores: Panserat, Stéphane; Blin, C; Medale, Francoise; Plagnes Juan, E; Breque, Jeanine; Krishnamoorthy, J; Kaushik, Sadasivam.
The enzyme glucokinase (GK) (EC 2.7.1.1) plays an important role in the control of glucose homeostasis. Qualitative and/or quantitative variations in GK enzyme have been postulated by previous studies to explain why dietary carbohydrate utilisation is lower in gilthead seabream (Sparus aurata) and rainbow trout (Oncorhynchus mykiss) than in common carp (Cyprinus carpio). In this study, we report the isolation and characterisation of a full-length cDNA coding for GK in these teleosts. Amino acid sequences derived from these cDNA clones are highly similar to other vertebrate GKs. These findings, including a detailed phylogenetic analysis, reveal that GK gene highly homologous to mammalian GK exists in these fish species with similar tissue specific...
Tipo: Text
Palavras-chave: Glucose phosphorylation; Dietary carbohydrate; Fish nutrition.
Ano: 2000
URL: http://archimer.ifremer.fr/doc/2000/publication-669.pdf

Nutritional regulation and tissue specificity of gene expression for proteins involved in hepatic glucose metabolism in rainbow trout (Oncorhynchus mykiss)
Provedor de dados: 5
Autores: Panserat, Stéphane; Plagnes Juan, E; Kaushik, Sadasivam.
Rainbow trout (Oncorhynchus mykiss) are known to use dietary carbohydrates poorly. One of the hypotheses to explain the poor utilisation of dietary glucose by these fish is a dysfunction in nutritional regulation of hepatic glucose metabolism. In this study, we obtained partial clones of rainbow trout cDNAs coding for a glucose transporter (Glut2), and for the enzymes 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (6PF-2K/F-2,6BPase), fructose-1,6-bisphosphatase (FBPase) and pyruvate kinase (PI(), Their deduced amino acid sequences were highly similar to those of mammals (up to 80% similarity), In a study of nutritional regulation, the Glut2 gene was highly expressed in the liver irrespective of the nutritional status of the trout, in agreement with...
Tipo: Text
Palavras-chave: Oncorhynchus mykiss; Trout; Bifunctional enzyme; Pyruvate kinase; Fructose bisphosphatase; Glucose transporter; Glucose metabolism; Liver metabolism.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-415.pdf