Consulta: creatorFacets:"Perini,A.P."
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Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient
Provedor de dados: 45
Autores: Resende,M.V.; Lucio,A.C.; Perini,A.P.; Oliveira,L.Z.; Almeida,A.O.; Alves,B.C.A.; Moreira-Filho,C.A.; Santos,I.W.; Hossepian de Lima,V.F.M.
The objective of the present study was to determine the sperm enrichment with X-bearing spermatozoa, after one centrifugation in a Percoll or OptiPrep continuous density gradient, using quantitative real-time polymerase chain reaction (qPCR) of sperm DNA and resultant in vitro-produced bovine embryos by PCR. Frozen/thawed sperm was layered on density gradients and the tubes were centrifuged. Supernatants were gently aspirated and the sperm recovered from the bottom of the tubes. Cleavage and blastocyst rates were determined through in vitro production of embryos and PCR was performed to identify the embryos' genetic sex. A difference in blastocyst rate was found in the Percoll treatment compared to OptiPrep (P&lt;0.05). The percentage of female embryos in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Sperm sexing; Centrifugation; Embryo sexing; QPCR; PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300002

Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production
Provedor de dados: 45
Autores: Lucio,A.C; Resende,M.V; Dernowseck-Meirelles,J.A.; Perini,A.P.; Oliveira,L.Z; Miguel,M.C.V; Carmo,A.S; Tomita,S.Y; Alves,B.C.A; Fazano,F.A.T.; Lima,V.F.M.H.
The purpose of this work was to associate the modified swim-up method with centrifugation in density gradient for the separation of X-bearing spermatozoa. Sperm viability and integrity were evaluated through the Trypan Blue/Giemsa staining method. Quality control of centrifuged spermatozoa was performed in in vitro produced embryos. The results were validated by the sex ratio of in vitro produced embryos using PCR by Y- specific sequences present in bovine male genomic DNA. After determining genetic sex of in vitro produced embryos, the results showed difference (P&lt;0.05) in deviation of sex ratio when comparing the control group (45.2% females) with the other spermatozoa selection procedures (60.6% females) (P&lt;0.05). The sperm selection methods are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Density gradient centrifugation; Embryo sex ratio; Percoll™; Swim-up.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300001

Induction of the acrosome reaction test to in vitro estimate embryo production in Nelore cattle
Provedor de dados: 45
Autores: Costa,M.Z.; Oliveira,L.Z.; Resende,M.V.; Lucio,A.C.; Perini,A.P.; Miguel,M.C.V.; Lima,V.F.M.H.
The effectiveness of induction of the acrosome reaction (AR) test as a parameter to in vitro estimate embryo production (IVP) in Nelore breed and the AR pattern by the Trypan Blue/Giemsa (TB) stain were evaluated. Frozen semen samples from ten Nelore bulls were submitted to AR induction and were also evaluated for cleavage and blastocyst rates. The treatments utilized for AR induction were: control (TALP medium), TH (TALP medium + 10μg heparin), TL (TALP medium + 100μg lysophosphatidylcholine) and THL (TALP medium + 10μg heparin + 100μg lysophosphatidylcholine). Sperm acrosomal status and viability were evaluated by TB staining at 0 and after 4h incubation at 38°C. The results obtained for AR presented a significant difference (P&lt;0.05) in the percentage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Fertility; Semen; Acrosome reaction; Fertilization.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400001