Consulta: creatorFacets:"Perrot, Hubert"
Registros recuperados: 8
Data/hora: 06/06/2024 03:29:27

Building of an immunosensor: How can the composition and structure of the thiol attachment layer affect the immunosensor efficiency?
Provedor de dados: 5
Autores: Briand, Elisabeth; Salmain, Michèle; Henry, Jean-marie; Perrot, Hubert; Compere, Chantal; Pradier, Claire-marie.
Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. (c) 2006 Elsevier B.V. All rights reserved.
Tipo: Text
Palavras-chave: QCM; IRRAS; Thiol attachment; Immunosensor; Mixed SAM' s.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1988.pdf

Development of a Mass Sensitive Quartz Crystal Microbalance (QCM)-Based DNA Biosensor Using a 50 MHz Electronic Oscillator Circuit
Provedor de dados: 5
Autores: Garcia-martinez, Gonzalo; Alonso Bustabad, Enrique; Perrot, Hubert; Gabrielli, Claude; Bucur, Bogdan; Lazerges, Mathieu; Rose, Daniel; Rodriguez-pardo, Loreto; Farina, Jose; Compere, Chantal; Arnau Vives, Antonio.
This work deals with the design of a high sensitivity DNA sequence detector using a 50 MHz quartz crystal microbalance (QCM) electronic oscillator circuit. The oscillator circuitry is based on Miller topology, which is able to work in damping media. Calibration and experimental study of frequency noise are carried out, finding that the designed sensor has a resolution of 7.1 ng/cm(2) in dynamic conditions (with circulation of liquid). Then the oscillator is proved as DNA biosensor. Results show that the system is able to detect the presence of complementary target DNAs in a solution with high selectivity and sensitivity. DNA target concentrations higher of 50 ng/mL can be detected.
Tipo: Text
Palavras-chave: DNA sequence detection; Quartz crystal microbalance; Electronic oscillator; Sensitivity; Frequency noise; Resolution.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00044/15487/12886.pdf

Quartz crystal microbalance, an efficient tool to design supramolecular structure on solid substrate
Provedor de dados: 5
Autores: Lazerges, Mathieu; Perrot, Hubert; Pailleret, Alain; Antoine, Elisabeth; Compere, Chantal.
Beyond interest of these step by step synthese of linear and branched DNA polymers, this work demonstrates that the quartz crystal microbalance is a sensitive tool to design supramolecular biostructures on solid substrate by following in situ thermodynamic and kinetics of successive reactions.
Tipo: Text
Ano: 2005
URL: http://archimer.ifremer.fr/doc/00089/19997/17660.pdf

Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum
Provedor de dados: 5
Autores: Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Compere, Chantal.
Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter...
Tipo: Text
Palavras-chave: Alexandrium Minutum; Avidin; Biosensor; Biotin; DNA; Quartz crystal miocrobalance; Self-assembled monolayer; Thiol.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00107/21810/19385.pdf

Biosensors for the detection and monitoring of Alexandrium minutum along the French coasts
Provedor de dados: 5
Autores: Antoine, Elisabeth; Compere, Chantal; Cosnier, Serge; Crassous, Marie-pierre; Derbal, Hassina; Erard-le Denn, Evelyne; Gondran, Chantal; Guilminot, Elodie; Lehaitre, Michel; Mazeas, Florence; Moreau, Hervé; Perrot, Hubert; Pradier, Claire-marie; Salmain, Michèle.
The microalgae Alexandrium minutum produces neurotoxins responsible for paralytic shellfish poisoning, which upon accumulation in shellfish represent a human health risk. This species recurrently forms toxic blooms in the Northern part of the French Brittany coast. At present, algae identification relies on tedious microscope observations mostly performed by skilled taxonomists in laboratories. Newly emerging fields of molecular taxonomy, nanoscale technology and biotechnology offer opportunities for the development of in-situ biosensors for the detection and the monitoring of toxic algae.
Tipo: Text
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00089/19986/17650.pdf

Direct and fast detection of Alexandrium minutum algae by using high frequency microbalance
Provedor de dados: 5
Autores: Sousa, Celia; Compere, Chantal; Dreanno, Catherine; Crassous, Marie-pierre; Gas, Fabienne; Baus, Beatrice; Perrot, Hubert.
In this paper, a simple detection of a toxic algae, Alexandrium minutum, was developed using highly sensitive quartz crystal microbalance. In terms of performance, compared with other conventional analytical tools, the main interest of our immunosensor is based on a fast and direct detection of these living cells. This system requires the use of one monoclonal antibody directed against the surface antigen of A. minutum. We demonstrate that the whole living and motile algae are caught and detected. The high specificity of the biosensor is also demonstrated by testing several other dinoflagellate species. The frequency shift is correlated to the A. minutum cells concentration. This simple system is potentially promising for environmental monitoring purposes.
Tipo: Text
Palavras-chave: Alexandrium minutum; Quartz microbalance; Microalgae; Immunosensor.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00194/30492/28897.pdf

Direct detection of toxic micro-algae Alexandrium minutum through high sensitive gravimetric sensors
Provedor de dados: 5
Autores: Bucur, Bogdan; Lazerges, Mathieu; Perrot, Hubert; Antoine, Elisabeth; Hamelin, Muriel; Compere, Chantal.
In this work QCM technologies were used to set up a DNA based biosensor dedicated to the detection of toxic micro-algae in the environment. Up to now, appropriate detection were not achieved for different reasons as the possibility of regeneration, the capability of multidetection or the detection of very low DNA target concentration. For these reasons different configurations were examined based on in-situ and fast regeneration, supramolecular chemistry of DNA and high sensitive gravimetric devices. In a first step an OligoDesoxyRibonucleotide 5’ tailed with a disulfide group (SH-ODN) designed to be a specific molecular probe for the toxic micro-algae Alexandrium minutum was grafted on the quartz surface resonators. Kinetics of probe immobilization and of...
Tipo: Text
Ano: 2008
URL: http://archimer.ifremer.fr/doc/00089/19996/17659.pdf

45-and 70-base DNA supramolecular polymerizations on quartz crystal microbalance biosensor
Provedor de dados: 5
Autores: Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Antoine, Elisabeth; Compere, Chantal.
Supramolecular polymerizations of 45- and 70- base DNAs on the surface of an in-situ time-resolved 27 MHz quartz crystal microbalance biosensor.
Tipo: Text
Palavras-chave: Sensor; Design; Kinetics; Immobilization; Hybridization.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-1710.pdf