Consulta: creatorFacets:"Piatti,Rosa Maria"
Registros recuperados: 7
Data/hora: 09/06/2024 07:30:03

Molecular subtyping of Campylobacter jejuni subsp. jejuni strains isolated from different animal species in the state of São Paulo, Brazil
Provedor de dados: 58
Autores: Scarcelli,Eliana; Piatti,Rosa Maria; Harakava,Ricardo; Miyashiro,Simone; Fernandes,Flora Maria de Campos; Campos,Fabíola Ribeiro; Francisco,Waldemar; Genovez,Margareth Élide; Richtzenhain,Leonardo José.
The objective of the present trial was to characterize genetically strains of Campylobacter jejuni subsp. jejuni isolated from humans and several animal sources (bovines, swine, dogs, primates, wild boars and poultry). A total of 828 different animal samples (feces, carcass, aborted fetus and hysterectomized uterus) were analysed by means of routine bacteriological methods, and 36 C. jejuni strains were isolated. Thirty strains of human fecal origin were obtained in clinical analysis laboratories in the city of São Paulo. The 66 C. jejuni strains isolated were submitted to genetic characterization. Primers based on fla A gene were used in a polymerase chain reaction (PCR) and amplified a fragment of the 702 bp. PCR products were evaluated by means of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campylobacter jejuni subtyping sequencing; Flagellin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400014

Leptospira spp detection by Polymerase Chain Reaction (PCR) in clinical samples of captive black-capped Capuchin monkey (Cebus apella)
Provedor de dados: 58
Autores: Scarcelli,Eliana; Piatti,Rosa Maria; Fedullo,José Daniel Luzes; Simon,Faiçal; Cardoso,Maristela Vasconcellos; Castro,Vanessa; Miyashiro,Simone; Genovez,Margareth Élide.
Leptospirosis is a widely distributed zoonosis that affects domestic and wild animals, and that has the man as the end point of its epidemiological chain. Leptospirosis diagnosis in primates is more difficult than in other animal species, as clinical signs and lesions are less evident and antibody response is detected only for short periods. The aim of this article was to describe the detection of Leptospira spp using polymerase chain reaction (PCR), in clinical samples from one captive black-capped Capuchin monkey (Cebus apella), which presented characteristics compatible with leptospirosis (jaundice and haemorrhagic kdney) in the macroscopic post-mortem examination. A friable kidney fragment and urine sample were cultured and submitted to experimental...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Nonhuman primate; PCR; Cebus apella.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200010

Brucella canis infection in dogs attended in veterinary clinics from patos, Paraíba state, Brazil
Provedor de dados: 58
Autores: Fernandes,Annielle Regina Fonseca; Azevedo,Sérgio Santos de; Piatti,Rosa Maria; Pinheiro,Eliana Scarcelli; Genovez,Margareth Élide; Azevedo,Adílio Santos de; Batista,Carolina de Sousa Américo; Alves,Clebert José.
To determine the frequency of anti-Brucella canis antibodies in dogs attended in veterinary clinics from Patos, Paraíba State, Brazil, as well as to identify risk factors and to isolate and identify the agent, 193 dogs were used. Agar gel immunodiffusion test (AGID) was used to detect B. canis antibodies in sera. Isolation of B. canis was carried out in blood and bone marrow from seropositive animals. Six animals tested seropositive in AGID, resulting in a frequency of 3.11%. B. canis was isolated from bone marrow of one seropositive animal, with confirmation by PCR. Lack of cleaning of the dog's environment was identified as risk factor (odds ratio = 7.91). This is the first report of isolation of B. canis in dogs from the Northeast region of Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial diseases; Canine brucellosis; Prevalence; Risk factors; Microbiological culture.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400023

Characterization of Aujeszky's disease virus isolates from South and Southeast Brazil by RFLP analysis
Provedor de dados: 58
Autores: Piatti,Rosa Maria; Ikuno,Alice Akimi; Cunha,Elenice Sequetin; D'Ambros,Régia; Gregori,Fábio; Soares,Rodrigo Martins; Cortez,Adriana; Richtzenhain,Leonardo José.
The genomic DNA of thirty strains of Aujeszky's disease virus (ADV) isolated in the South and Southeast regions of Brazil from 1982 to 1996 were characterized by restriction endonuclease analysis with BamHI. Twenty seven strains were isolated from pigs, 1 from cattle, 1 from cat and 1 from dog. Using a systematization previously described, the 30 ADV strains could be classified as genomic types I (n = 2) and II (n = 28). Genomic type III was not observed. In this first study of genomic type characterization of brazilian ADV strains, we could demonstrate the occurence in Brazil of the genomic types I and II, with a large predominance of genomic type II.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aujeszky's disease virus; Swine; Genomic RFLP; Brazil.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200015

Seroprevalence of antibodies to Chlamydophila abortus in ovine in the State of Alagoas, Brazil
Provedor de dados: 58
Autores: Pinheiro Junior,José Wilton; Mota,Rinaldo Aparecido; Piatti,Rosa Maria; Oliveira,Andréa Alice da Fonseca; Silva,Aline Melo da; Abreu,Sílvio Romero de Oliveira; Anderlini,Giulliano Aires; Valença,Rômulo Menna Barreto.
The goal of this study was to perform a seroepidemiological investigation and to identify risk factors associated with infection of Chlamydophila abortus of sheep herds in the Brazilian state of Alagoas. The study was conducted with samples of 274 ewes with ages equal to or higher than 24 months in 25 herds and in 23 towns located in three regions of the state (Sertão, Agreste and Eastern Alagoas). Anti-C. abortus antibodies were detected using the microcomplement fixation test. The risk factors, were determined based on questionnaires consisting of objective questions, about the farmer and general characteristics of the herd like size, sanitary situation and reproductive management. Among 274 sera samples analyzed for C. abortus, 59 (21.5%) were positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnosis; Chlamydiosis; Serum epidemiology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200015

Detection of CDT toxin genes in Campylobacter spp. strains isolated from broiler carcasses and vegetables in São Paulo, Brazil
Provedor de dados: 58
Autores: Carvalho,Aline Feola de; Silva,Daniela Martins da; Azevedo,Sergio Santos; Piatti,Rosa Maria; Genovez,Margareth Elide; Scarcelli,Eliana.
Campylobacteriosis is a worldwide distributed zoonosis. One of the main virulence factors related to Campylobacter spp. in animals and humans is the cytolethal distending toxin (CDT), encoded by three adjacent genes (cdtA, cdtB, cdtC). The occurrence of Campylobacter spp. in samples of vegetables has not been reported in Brazil yet, and has seldom been described in the international literature. The detection of CDT in these strains has not been reported, either. The objectives of the present study were to determine the occurrence of Campylobacter spp. strains carrying virulence factors in samples of poultry and vegetables (lettuce and spinach) from different points of sale, thus verifying if vegetables are as an important vehicle for potentially virulent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campylobacter spp.; Cytolethal distending toxin (CDT); Broiler carcasses; Vegetables; Multiplex-PCR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300005

Sinais clínicos e ocorrência de anticorpos anti-Chlamydophila abortus em ovinos de São Paulo e Minas Gerais
Provedor de dados: 65
Autores: Rossi,Rodolfo Santos; Rizzo,Huber; Piatti,Rosa Maria; Gregory,Lilian.
A Chlamydophila abortusi, anteriormente conhecida como Chlamydia psittaci sovovar 1, é uma bactéria Gram negativa, intracelular obrigatória. Esse micro-organismo é frequentemente encontrado em distúrbios reprodutivos em ovinos, bovinos e caprinos, sendo o aborto epizoótico dos bovinos e o aborto enzoótico dos ovinos e caprinos as manifestações mais importantes. Considerando-se o pouco material literário a respeito da clamidofilose no Brasil, a pesquisa teve como objetivo determinar a presença de anticorpos fixadores de complemento anti-Chlamydophila abortusi, correlacionando os resultados obtidos com achados no exame clínico e histórico dos animais, além de alterações nos índices zootécnicos, em especial na esfera reprodutiva, tais como alto índice de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovinos; Aborto; Chlamydophila abortus; Aborto enzoótico dos ovinos; Clamidofilose.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012001100017