Consulta: creatorFacets:"Piel, Damien"
Registros recuperados: 4
Data/hora: 01/06/2024 03:36:06

Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations
Provedor de dados: 5
Autores: Bruto, Maxime; Labreuche, Yannick; James, Adele; Piel, Damien; Chenivesse, Sabine; Petton, Bruno; Polz, Martin F.; Le Roux, Frederique.
Diseases of marine animals caused by bacteria of the genus Vibrio are on the rise worldwide. Understanding the eco-evolutionary dynamics of these infectious agents is important for predicting and managing these diseases. Yet, compared to Vibrio infecting humans, knowledge of their role as animal pathogens is scarce. Here we ask how widespread is virulence among ecologically differentiated Vibrio populations, and what is the nature and frequency of virulence genes within these populations? We use a combination of population genomics and molecular genetics to assay hundreds of Vibrio strains for their virulence in the oyster Crassostrea gigas, a unique animal model that allows high-throughput infection assays. We show that within the diverse Splendidus...
Tipo: Text
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00452/56370/57967.pdf

Selection of  Vibrio crassostreae  relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells
Provedor de dados: 5
Autores: Piel, Damien; Bruto, Maxime; James, Adele; Labreuche, Yannick; Lambert, Christophe; Janicot, Adrian; Chenivesse, Sabine; Petton, Bruno; Wegner, K. Mathias; Stoudmann, Candice; Blokesch, Melanie; Le Roux, Frederique.
Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae has been repeatedly isolated from diseased animals and the majority of the strains have been demonstrated to be virulent for oysters. In this study we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by V. crassostreae while oysters, at an adult stage, were reservoirs of this virulent population. The pathogenicity of V. crassostreae depends on a novel transcriptional regulator, which activates the bidirectional promoter of a Type 6 Secretion System (T6SS) genes cluster. Both the T6SS and a second chromosomal virulence factor, r5.7, are...
Tipo: Text
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00510/62129/66343.pdf

Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios
Provedor de dados: 5
Autores: Wegner, K. Mathias; Piel, Damien; Bruto, Maxime; John, Uwe; Mao, Zhijuan; Alunno-bruscia, Marianne; Petton, Bruno; Le Roux, Frederique.
Bacteria of the Vibrio genus are the most predominant infectious agents threatening marine wildlife and aquaculture. Due to the large genetic diversity of these pathogens, the molecular determinants of Vibrio virulence are only poorly understood. Furthermore, studies tend to ignore co-evolutionary interactions between different host populations and their locally encountered Vibrio communities. Here, we explore the molecular targets of such co-evolutionary interactions by analyzing the genomes of nine Vibrio strains from the Splendidus-clade showing opposite virulence patterns towards two populations of Pacific oysters introduced into European Wadden Sea. By contrasting Vibrio phylogeny to their host specific virulence patterns, we could identify two core...
Tipo: Text
Palavras-chave: Virulence; Host-pathogen interaction; Local adaptation; Biological invasion; Emerging disease; Wadden Sea.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00512/62409/66669.pdf

Évolution de la virulence de V. crassostreae en lien avec l’huître en tant qu’hôte et les phages en tant que prédateurs
Provedor de dados: 5
Autores: Piel, Damien.
Understanding the ecological and evolutionary dynamics of infectious agents is important for diagnosing, predicting and preventing diseases in farmed and wild species. This project was aimed at studying the evolution of V. crassostreae virulence in relation to the oyster as a host and to the phages as predators. We identified the molecular mechanisms leading to the adaptation of vibrios to oysters. We demonstrated the emergence of virulent strains of V. crassostreae in the area impacted by the juvenile oyster mortality syndrome. The emergence of more virulent V. crassostreae strains is linked to the acquisition of a plasmid encoding a type 6 secretion system responsible for lethal activity towards oyster hemocytes. This project also provides knowledge on...
Tipo: Text
Palavras-chave: Vibrio; Interactions hôte/parasite; Emergence de pathogènes; Bactériophages; V. crassostreae; Mécanismes moléculaires; Vibrio; Host/parasite interactions; Pathogen emergence; Bacteriophages.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00690/80203/83289.pdf