Consulta: creatorFacets:"Pio-Ribeiro,Gilvan"
Registros recuperados: 7
Data/hora: 02/06/2024 17:48:45

Evaluation of peanut genotypes for resistance to Tomato spotted wilt virus by mechanical and thrips inoculation
Provedor de dados: 86
Autores: Nascimento,Luciana Cordeiro do; Pensuk,Viboon; Costa,Nivânia Pereira da; Assis Filho,Francisco Miguel de; Pio-Ribeiro,Gilvan; Deom,Carl Michael; Sherwood,John.
The objective of this work was to evaluate the reactions of three peanut breeding lines (IC-10, IC-34, and ICGV 86388) to Tomato spotted wilt virus (TSWV) by mechanical and thrips inoculation, under greenhouse conditions, and compare them to the reactions of cultivars SunOleic, Georgia Green, and the breeding line C11-2-39. TSWV infection by mechanical inoculation was visually assessed using an index ranging from 0 (no symptoms) to 4 (apical death). Enzyme-linked immunosorbent assay was used to confirm TSWV infection from both mechanical and thrips inoculations. IC-10, IC-34, ICGV 86388, and C11-2-39 were more resistant than the cultivars SunOleic and Georgia Green based on mechanical inoculation. Upon thrips inoculation only IC-34 and ICGV-86388 were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachis hypogaea; TSWV; Mechanical transmission; Thrips transmission.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000600006

Production of polyclonal antisera using recombinant coat proteins of Grapevine leafroll-associated virus 2 and Grapevine virus B
Provedor de dados: 86
Autores: Radaelli,Paula; Fajardo,Thor Vinícius Martins; Nickel,Osmar; Eiras,Marcelo; Pio-Ribeiro,Gilvan.
The objective of this work was to produce and characterize specific antisera against Brazilian isolates of Grapevine leafroll-associated virus 2 (GLRaV-2) and Grapevine virus B (GVB), developed from expressed coat proteins (CPs) in Escherichia coli, and to test their possible use for the detection of these two viruses in diseased grapevines. The coat protein (CP) genes were RT-PCR-amplified, cloned and sequenced. The CP genes were subsequently subcloned, and the recombinant plasmids were used to transform E. coli cells and express the coat proteins. The recombinant coat proteins were purified, and their identities were confirmed by SDS-PAGE and Western blot and used for rabbit immunizations. Antisera raised against these proteins were able to recognize the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitis; GLRaV-2; GVB; Indirect ELISA; Recombinant protein; Western blot.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000020

Desempenho agronômico de videiras com e sem sintomas de viroses, e comparação molecular de isolados virais
Provedor de dados: 86
Autores: Nascimento,Monique Bezerra; Fajardo,Thor Vinícius Martins; Eiras,Marcelo; Czermainski,Ana Beatriz Costa; Nickel,Osmar; Pio-Ribeiro,Gilvan.
Resumo: O objetivo deste trabalho foi avaliar os efeitos de viroses em videiras sintomáticas e assintomáticas sobre as variáveis agronômicas relacionadas ao vigor das plantas e à qualidade enológica da uva, e comparar os isolados virais obtidos nessas duas condições. Realizaram-se dois experimentos com quatro cultivares. Todas as plantas foram indexadas, por meio da reação em cadeia da polimerase via transcrição reversa (RT-PCR) em tempo real, quanto à provável ocorrência dos seguintes vírus: Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine leafroll-associated virus (GLRaV-1 ao -4, GLRaV-4 estirpe 5), Grapevine rupestris stem pitting-associated virus (GRSPaV) e Grapevine fleck virus (GFkV). As variáveis avaliadas foram:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grapevine; Vitis; Complexo do lenho rugoso; Enrolamento-da-folha; PCR em tempo real.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000700541

Stock indexing and Potato virus Y elimination from potato plants cultivated in vitro
Provedor de dados: 63
Autores: Nascimento,Luciana Cordeiro; Pio-Ribeiro,Gilvan; Willadino,Lilia; Andrade,Genira Pereira.
Potato cultivars (Solanum tuberosum L.) have shown degeneration or run out caused by viruses after several cycles of propagation using seed tubers from commercial fields. This work reports the occurrence of single and mixed infections of four potato viruses in Paraíba-Brazil and presents a method for Potato virus Y (PVY) elimination, by using thermo-and chemotherapies. Plants of potato cv. Baraka were tested by direct antigen coating ELISA. Antisera against PVY, Potato virus X (PVX), Potato virus S (PVS), and Potato leafroll virus (PLRV) were used. Materials with positive reaction to PVY were treated for virus elimination. Single node cuttings (1.0 cm length) were excised and inoculated in Murashige &amp;amp; Skoog (MS) medium, supplemented with 1.0 mg L-1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum tuberosum; Virus-free clones; Thermotherapy; Chemotherapy.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000300017

Análise filogenética de potyvírus causando endurecimento dos frutos do maracujazeiro no Nordeste do Brasil
Provedor de dados: 102
Autores: Nascimento,Ana Verônica S.; Souza,Ana R. R.; Alfenas,Poliane F.; Andrade,Genira P.; Carvalho,Murilo G.; Pio-Ribeiro,Gilvan; Zerbini,F. Murilo.
Amostras foliares de plantas de maracujá-amarelo (Passiflora edulis f. flavicarpa) com sintomas típicos de endurecimento dos frutos foram coletadas nos estados de Pernambuco, Paraíba e Sergipe. A infecção viral foi comprovada por meio de teste sorológico e gama de hospedeiros. Os seis isolados virais obtidos foram capazes de infetar várias espécies testadas, porém apresentando diferenças na intensidade dos sintomas induzidos nessas hospedeiras. Teste de ELISA indireto demonstrou que os isolados obtidos a partir de plantas de maracujá são sorologicamente relacionados entre si e com o potyvírus Cowpea aphid-borne mosaic virus (CABMV). A seqüência de aminoácidos da proteína capsidial foi determinada para os seis isolados. A comparação dessas seqüências com as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caracterização molecular; Proteína capsidial; Maracujá; Passiflora edulis f. flavicarpa; PWV; CABMV.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000400003

Incidência de vírus em videiras no Nordeste brasileiro e caracterização molecular parcial de isolados virais locais
Provedor de dados: 65
Autores: Catarino,Aricléia de Moraes; Fajardo,Thor Vinícius Martins; Pio-Ribeiro,Gilvan; Eiras,Marcelo; Nickel,Osmar.
Os objetivos deste trabalho foram identificar as espécies virais presentes em vinhedos comerciais de duas regiões do Nordeste do Brasil e realizar a caracterização molecular parcial de isolados de três espécies virais. A diagnose foi realizada por meio de RT-PCR em tempo real para a detecção de Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine leafroll-associated virus 2, 3 e 4 (GLRaV-2, -3 e -4), Grapevine fleck virus (GFkV), Grapevine rupestris vein feathering virus (GRVFV) e Grapevine fanleaf virus (GFLV). Exceto para GFLV, os vírus avaliados estão amplamente disseminados nas áreas amostradas, frequentemente em altas incidências e em infecções múltiplas, de até 98% e 76,4%, na Zona da...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proteína capsidial; Levantamento; Detecção; Variabilidade; Vitis.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000300379

Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil
Provedor de dados: 65
Autores: Fajardo,Thor Vinícius Martins; Nascimento,Monique Bezerra; Eiras,Marcelo; Nickel,Osmar; Pio-Ribeiro,Gilvan.
ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Diagnosis; Sequencing; Ilarvirus; PNRSV; ApMV; ArMV.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001202197