Consulta: creatorFacets:"Polo,Érico Macedo"
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Optimization of heterologous microsatellites in Piracanjuba
Provedor de dados: 86
Autores: Carmo,Fausto Moreira da Silva; Polo,Érico Macedo; Silva,Marcos Antônio da; Yazbeck,Gabriel de Menezes.
Abstract: The objective of this work was to evaluate 41 microsatellite markers for heterologous amplifications in piracanjuba (Brycon orbignyanus). Some markers were tested for the first time. Loci were optimized for PCR conditions and applied to a sample of 49 individuals. Thirty-one loci resulted in PCR product formation, whereas ten loci yielded intelligible polymorphic patterns in the evaluated sample and can be used for amplifications in this species. From the evaluated markers, four loci (BoM1, BoM13, Bh6, and Bh16) are valid to be applied in the study of piracanjuba.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brycon orbignyanus; Aquaculture; Hatchery; Molecular markers.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015001201236