Consulta: creatorFacets:"RIOS,MARIANA"
Registros recuperados: 2
Data/hora: 13/06/2024 19:51:51

A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct
Provedor de dados: 48
Autores: RIOS,MARIANA; OJEDA,SUANY; VELASQUEZ,LUIS A; MAISEY,KEVIN; CROXATTO,HORACIO B.
Estradiol accelerates oviductal embryo transport in the rat through changes of genomic expression in oviductal cells. However, the genes involved are unknown. We used a differential display by reverse transcription-polymerase chain reaction to detect estradiol (E2)-dependent genes in the rat oviduct. Rats on day 2 of pregnancy were untreated or treated with 10 µg of E2 and the oviducts were extracted at 30, 180 and 360 min later and used to isolate RNA. Products of reverse transcriptase-PCR, made with pairs of arbitrary and oligo-deoxythymidine primers, were separated on denaturing polyacrylamide gels and candidate bands were excised and reamplified. Truly positive cDNA fragments determined by a single strand conformation polymorphism assay were cloned and...
Tipo: Journal article
Palavras-chave: Differential display; Estradiol; Gene; MRNA; Oviduct.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100011

In vivo expression of ß-galactosidase by rat oviduct exposed to naked DNA or messenger RNA
Provedor de dados: 48
Autores: RIOS,MARIANA; VENEGAS,ALEJANDRO; CROXATTO,HORACIO B.
Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (&lt;A HREF="#rios97"&gt;Ríos et al., 1997&lt;/A&gt;). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 µg of pure ß-galactosidase (ß-gal) mRNA, 50 µg of pure DNA from the reporter gene ß-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the ß-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-ß-D-galactopyranoside as a substrate. The administration of ß-gal mRNA and pSVBgal...
Tipo: Journal article
Palavras-chave: SS-galactosidase; Gene transfer; Oviduct.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300007