Consulta: creatorFacets:"RIVAS,MARIELLA"
Registros recuperados: 2
Data/hora: 01/06/2024 08:11:39

Biochemical characterization and inhibitory effects of dinophysistoxin-1, okadaic acid and microcystine l-r on protein phosphatase 2a purified from the mussel Mytilus chilensis.
Provedor de dados: 48
Autores: RIVAS,MARIELLA; GARCÍA,CARLOS; LIBERONA,JOSÉ L; LAGOS,NÉSTOR.
Protein phosphatases are involved in many cellular processes. One of the most abundant and best studied members of this class is protein phosphatase type-2A (PP2A). In this study, PP2A was purified from the mussel Mytilus chilensis. Using both SDS-PAGE and size exclusion gel filtration under denaturant conditions, it was confirmed that the PP2A fraction was essentially pure. The isolated enzyme is a heterodimer and the molecular estimated masses of the subunits are 62 and 28 kDa. The isolated PP2A fraction has a notably high p-NPP phosphatase activity, which is inhibited by NaCl. The hydrolytic p-NPP phosphatase activity is independent of the MgCl2 concentration. The time courses of the inhibition of the PP2A activity of p-NPP hydrolysis by increasing...
Tipo: Journal article
Palavras-chave: Chile; Diarrhetic Shellfish Poisoning (DSP); Mussel; Mytilus chilensis; Protein Phosphatase 2A; Microcystine L-R.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000300005

A Lux-like Quorum Sensing System in the Extreme Acidophile Acidithiobacillus ferrooxidans
Provedor de dados: 48
Autores: RIVAS,MARIELLA; SEEGER,MICHAEL; HOLMES,DAVID S; JEDLICKI,EUGENIA.
The genome of the acidophilic, proteobacterium Acidithiobacillus ferrooxidans, contains linked but divergently oriented genes, termed afeI and afeR, whose predicted protein products are significantly similar to the LuxI and LuxR families of proteins. A possible promoter and Lux box are predicted upstream of afeI. A cloned copy of afeI, expressed in E. coli, encodes an enzyme that catalyzes the production of a diffusible compound identified by gas chromatography and mass spectrometry as an unsubstituted N-acyl homoserine lactone (AHL) of chain length C14. This AHL can be detected by a reporter strain of Sinorhizobium meliloti Rm41 suggesting that it is biologically active. The reporter strain also responds to extracts of the supernatant of A. ferrooxidans...
Tipo: Journal article
Palavras-chave: A. ferrooxidans; Quorum-sensing; LuxI and LuxR; Lux box; Homoserine lactone.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200018