Consulta: creatorFacets:"Rao,Adhikarla Suryanarayana"
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Data/hora: 01/06/2024 15:23:03

Effect of picloram, additives and plant growth regulators on somatic embryogenesis of Phyla nodiflora (L.) Greene
Provedor de dados: 52
Autores: Ahmed,Abdul Bakrudeen Ali; Rao,Adhikarla Suryanarayana; Rao,Mandali Venkateswara; Taha,Rosna Mat.
The present study describes the plant regeneration via somatic embryogenesis in suspension culture derived from the leaf and stem explants of Phyla nodiflora. The medium type, plant growth regulators, complex extract (coconut milk and malt extract) and anti-oxidant (activated charcoal, ascorbic acid, Polyvinylpyrrolidone and citric acid) markedly influenced the embryo regeneration of P. nodiflora. MS with 2,4-D and activated charcoal (10 mg/L) gave the highest stimulation of embryogenic callus growth. Optimized callus was transfered into suspension culture, which showed the globular, heart shaped embryos in MS with 2,4-D + BA + picloram (0.1 mg/L), coconut milk (10 ml/L), citric acid (100 mg/L) on 6th subcultures. Further development stages such as torpedo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Somatic embryogenesis (SEs); Picloram; Ascorbic acid; Coconut milk; Plant growth regulators; Murashige and Skoog medium.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100002