Consulta: creatorFacets:"Romanha,A.J."
Registros recuperados: 3
Data/hora: 05/06/2024 06:47:39

In vitro activity of hypnophilin from Lentinus strigosus: a potential prototype for Chagas disease and leishmaniasis chemotherapy
Provedor de dados: 56
Autores: Souza-Fagundes,E.M.; Cota,B.B.; Rosa,L.H.; Romanha,A.J.; Corrêa-Oliveira,R.; Rosa,C.A.; Zani,C.L.; Teixeira-Carvalho,A.; Martins-Filho,O.A.
Hypnophilin and panepoxydone, terpenoids isolated from Lentinus strigosus, have significant inhibitory activity onTrypanosoma cruzi trypanothione reductase (TR). Although they have similar TR inhibitory activity at 10 μg/mL (40.3 μM and 47.6 μM for hypnophilin and panepoxydone, respectively; ~100%), hypnophilin has a slightly greater inhibitory activity (~71%) on T. cruzi amastigote (AMA) growth in vitro as well as on in vitro phytohemagglutinin (PHA)-induced peripheral blood mononuclear (PBMC) proliferation (~70%) compared to panepoxydone (69% AMA inhibition and 91% PBMC inhibition). Hypnophilin and panepoxydone at 1.25 μg/mL had 67% inhibitory activity onLeishmania (Leishmania) amazonensis amastigote-like (AMA-like) growth in vitro. The panepoxydone...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chagas disease; Leishmaniasis; Chemotherapy; Immunomodulation; Hypnophilin; Lentinus strigosus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100007

Endothelin-1 receptors play a minor role in the protection against acute Trypanosoma cruzi infection in mice
Provedor de dados: 56
Autores: Roffê,E.; Souza,A.L.S.; Machado,P.P.; Barcelos,L.S.; Romanha,A.J.; Mariano,F.S.; Silva,J.S.; Machado,C.R.; Tanowitz,H.B.; Teixeira,M.M.
Chagas' disease, caused by the protozoan Trypanosoma cruzi, is a major cause of cardiovascular disability in countries where it is endemic. Damage to the heart microvasculature has been proposed to be an important factor in the pathogenesis of heart dysfunction. Endothelin-1 (ET-1) is a potent vasoconstrictor and exerts its effects via specific ET A and ET B receptors. A few studies have suggested a role for ET-1 and its receptors in the pathogenesis of Chagas' disease. We investigated the effects of treatment with bosentan, an ET A/ET B receptor antagonist, on the course of T. cruzi infection (Y strain) in C57Bl/6 mice. Treatment with bosentan (100 mg kg-1 day-1) was given per os starting day 0 after infection until sacrifice. Bosentan significantly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Endothelin; Myocarditis; Chemokines.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300015

An improved HPLC method for the quantitation of 6-mercaptopurine and its metabolites in red blood cells
Provedor de dados: 56
Autores: Oliveira,B.M.; Romanha,A.J.; Alves,T.M.A.; Viana,M.B.; Zani,C.L.
A procedure is described for the rapid determination of the intra-erythrocyte concentration of 6-mercaptopurine (6-MP) and its metabolites, 6-thioguanine nucleotides (6-TGN) and 6-methylmercaptopurine (6-MMP). Erythrocytes (8 x 10(8) cells) in 350 µl Hanks solution containing 7.5 mg dithiothreitol were treated with 50 µl 70% perchloric acid. The precipitate was removed by centrifugation (13,000 g) and the supernatant hydrolyzed at 100°C for 45 min. After cooling, 100 µl was analyzed directly by HPLC using a Radialpack Resolve C18 column eluted with methanol-water (7.5:92.5, v/v) containing 100 mM triethylamine. 6-TG, 6-MP and the hydrolysis product of 6-MMP, 4-amino-5-(methylthio)carbonyl imidazole, were monitored at 342, 322 and 303 nm using a Shimadzu...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute lymphoblastic; Ukemia; Chemotherapy; 6-Mercaptopurine; Compliance; Children.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500004