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Development of a mreB-targeted real-time PCR method for the quantitative detection of Vibrio harveyi in seawater and biofilm from aquaculture systems
Provedor de dados: 5
Autores: Mougin, Julia; Roquigny, Roxane; Travers, Marie-agnes; Grard, Thierry; Bonnin-jusserand, Maryse; Le Bris, Cédric.
Vibrio harveyi is a particularly problematic Gram-negative bacterium because it can form biofilms on aquaculture facility surfaces, leading to resistance of bacteria against antibiotics and water sanitizers. A SYBR Green I quantitative real-time PCR method was developed to detect V. harveyi directly from environmental samples, including seawater and biofilm. Specific primers targeting the mreB gene were designed. The exclusivity and inclusivity of the newly designed primers were evaluated using a panel of 85 bacteria: 58 V. harveyi from multiples origins and 27 non-V. harveyi isolates, and compared with two pairs of primers targeting the topA and toxR genes that were designed previously. All sets of primers were able to distinguish V. harveyi from closely...
Tipo: Text
Palavras-chave: Vibrio harveyi; Quantitative detection; MreB gene; Seawater; Biofilm; Aquaculture.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00622/73365/72542.pdf