Consulta: creatorFacets:"Rosa,Paulo Roberto Antunes da"
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Data/hora: 09/06/2024 18:21:10

Grb10 characterization in bovine cumulus oocyte complexes from different follicle sizes
Provedor de dados: 65
Autores: Rosa,Paulo Roberto Antunes da; Bohrer,Rodrigo Camponogara; Ludke,Charles Alencar; Cesaro,Matheus Pedroti De; Pereira,Gabriel Ribas; Mondadori,Rafael Gianela; Antoniazzi,Alfredo Quites; Gonçalves,Paulo Bayard Dias.
The objective of this study was to investigate the mRNA expression and protein localization of Grb10 gene in bovine cumulus-oocyte complexes (COCs) from different follicle sizes. Firstly, it was investigated the mRNA expression to correlate with maturation rates. COCs from follicles at 1-3, 4-6, 6-8 and &amp;gt;8mm were used to evaluate Grb10 gene expression by qRT-PCR assay and nuclear maturation rates. It was observed that more competent oocytes (from follicles at 6-8 and &amp;gt;8mm; P&amp;gt;0.05), had lower Grb10 mRNA expression levels when compared to the oocytes from follicles at 1-3 and 4-6mm (P&amp;gt;0.05). After it was performed an immunofluorescence analysis in COCs from different follicle sizes (1-3, 4-6, 6-8 and &amp;gt;8mm) to investigate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: : mRNA; Protein; Maturation; Oocyte; Grb10.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500898